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Amersham Pharmacia Biotech AKTA design pH/C-900, Руководство пользователя

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Amersham Pharmacia Biotech AKTA design pH/C-900 Скачать руководство пользователя страница 24

A.2 pH electrode

The pH electrode is of the sealed
combination double junction type.
It contains a sealed Ag/AgCl
reference which cannot be refilled,
an internal electrolyte bridge of 4M
KCl saturated with Ag/AgCl, an
outer electrolyte bridge of 1M
KNO

3

, an annular ceramic

reference junction and a low profile
pH membrane. The pH electrode is
delivered with a transparent cover.

The flow cell should not be used
with any other pH electrode.

A.3 Conductivity cell

The flow cell has two cylindrical titanium electrodes positioned in
the flow path of the cell. An alternating voltage is applied between
the electrodes and the resulting current is measured and used to
calculate the conductivity of the eluent. The monitor controls the AC
frequency and increases it with increasing conductivity between 50
Hz and 50 kHz giving maximum linearity and true conductivity
values.

The conductivity is automatically calculated by multiplying the
measured conductance by the flow cell’s cell constant. The cell
constant is pre-calibrated on delivery but can be measured with a
separate calibration procedure. This procedure is described in
Reference information section B.2.

One of the electrodes has a small temperature sensor for measuring
the temperature of the eluent in the flow cell. Temperature variations
influence the conductivity and in some applications, when highly
precise conductivity values are required, it is possible to program a
temperature compensation factor that recalculates the conductivity
to a set reference temperature.

Monitor pH/C-900

User Manual 18-1120-06 Edition AC

20

Eluent

Temperature sensor

Sealing

Internal electrolyte
bridge containing
4M KCl saturated
with AgCl

Silicone sealing

Internal annular
coaxial ceramic
reference junction

External electrolyte
bridge containing
1M KNO

3

O-ring

Glass electrode
containing diluted,
buffered KCl

External annular
coaxial ceramic
reference junction

O-ring

Ag/AgCl reference
electrode

A 

Reference information

18-1120-06_AC.qxd  1999-04-14 14:16  Sida 20 (1,1)

Содержание AKTA design pH/C-900

Страница 1: ...User Manual 18 1120 06 Monitor pH C 900 18 1120 06_AC qxd 1999 04 14 14 16 Sida 1 1 1...

Страница 2: ...Germany Amersham Pharmacia Biotech AB 1999 All rights reserved Important user information Meaning Consult the instruction manual to avoid personal injury or damage to the product or other equipment WA...

Страница 3: ...ivity values 8 3 5 Setting conductivity scale 8 3 6 Calibrating pH 9 3 7 Calibrating conductivity 11 3 8 Using an external chart recorder 11 3 9 Storage and shut down 12 3 10 Restart after power failu...

Страница 4: ...2 6 Calibrating the temperature sensor 24 B 2 7 Setting up the temperature display 25 B 2 8 Selecting language 25 B 2 9 Setup unit number 25 B 2 10 Setup display angle 25 B 3 Setting and using the al...

Страница 5: ...o not use in a dusty atmosphere or close to spraying water Operate in accordance with local safety instructions pH C 900 Monitor pH C 900 User Manual 18 1120 06 Edition AC 1 WARNING The instrument mus...

Страница 6: ...strument to ensure that the ventilation inlet in the front is not blocked 2 3 Installing the conductivity cell 1 Place the conductivity cell in a suitable place for example on the shelf of the Monitor...

Страница 7: ...ss bulb in buffer for 30 minutes 3 Remove the dummy electrode from the flow cell and store it in the flow cell holder 4 Carefully insert the electrode in the flow cell Tighten the nut by hand to secur...

Страница 8: ...measurements 2 Set the recorder to 0 1 V or 4 20 mA input full scale 2 6 Connecting to communication link The monitor can be used in KTAexplorer and KTApurifier and can be controlled from a PC runnin...

Страница 9: ...l constant is shown on the packaging Retain the packaging in case the conductivity cell constant needs to be re entered Note The measured temperature is the temperature in the conductivity flow cell w...

Страница 10: ...p 3 2 Menu selection and settings Moving between menus A specific menu is selected by turning the front selection dial clockwise or counterclockwise When the required menu is visible the menu or selec...

Страница 11: ...verview Monitor pH C 900 User Manual 18 1120 06 Edition AC 7 Set Cond Scale 100 50 00mS cm Calibrate pH 7 00 10 00 Set pH Analogue Out Check Alarm Timer 00 30 52 Setup Cond Temp pH 25 4 22 9 C 11 50 4...

Страница 12: ...obtain a usable output signal for a recorder or to view the conductivity as a percentage of buffer B it is possible to set a 0 to 100 range This can be done with reference to the buffers used or by se...

Страница 13: ...he procedure used with the electrode removed from the flow cell Note The Monitor must be unlocked if connected to a UNICORN control system 1 Remove the pH electrode from the flow cell and immerse the...

Страница 14: ...d be replaced An electrode is still usable at lower slopes and higher asymmetry potentials but the response will be slower and the accuracy diminished Calibrating with the electrode in the flow cell W...

Страница 15: ...utput connectors For the conductivity signal 0 represents 0 V and 100 represents 1 V as set under main menus Set Cond scale 0 and Set Cond scale 100 For the pH signal the full scale and zero level has...

Страница 16: ...of the electrode in buffer with a 1 1 mixture of pH 4 buffer and 1 M KNO3 overnight 3 10 Restart after power failure If the power supply to the instrument is interrupted the instrument automatically r...

Страница 17: ...lectrode first in 0 1 M HCl then in 0 1 M NaOH and again in 0 1 M HCl Each immersion is for a 5 minute period Rinse electrode tip in distilled water between each solution Oil or Grease Films Wash elec...

Страница 18: ...3 Rinse thoroughly with 50 ml de ionised water Note If the flow cell is totally blocked the blockage can be broken using a thin needle or a piece of string with a diameter less than 0 8 mm 4 6 Changi...

Страница 19: ...mbrane has dried out the electrode may be restored by soaking it in buffer overnight 3 Clogged liquid junction Refer to section 4 3 Cleaning the pH electrode Incorrect unstable pH reading 1 Check that...

Страница 20: ...rganic solvents Mount the dummy electrode instead 13 Clogged liquid junction Refer to section 4 3 Cleaning the pH electrode pH values vary with varied 1 Replace the pH electrode back pressure Conducti...

Страница 21: ...e 1 V full scale 3 Check the conductivity scaling and pH scaling see menus Set cond scale 100 section 3 5 Set cond scale 0 section 3 5 and Using an external chart recorder section 3 8 No text on the f...

Страница 22: ...nd Check that the conductivity cell is connected condition Diff cond 0 The difference between 0 and 100 must be at least 100 is too small 0 1 mS cm Recalibrate Only allowed Unit number cannot be chang...

Страница 23: ...Function pH Ground Signal ground pH Probe Connection to pH electrode standard BNC socket Conductivity Connection to conductivity flow cell Flow Cell 9 pole D sub connector Analog out 0 1V 2 separate r...

Страница 24: ...lated by multiplying the measured conductance by the flow cell s cell constant The cell constant is pre calibrated on delivery but can be measured with a separate calibration procedure This procedure...

Страница 25: ...ess OK 8 A test of the instrument buzzer is performed press OK B 2 Setup menu B 2 1 Setting up conductivity temperature compensation The conductivity in a buffer is temperature dependent To relate con...

Страница 26: ...When adjusting the cell constant from UNICORN select System Control System Calibrate and then select CondCalib Note The conductivity temperature compensation must not be used when adjusting the cell c...

Страница 27: ...p press OK 2 Select sub menu Setup Conductivity press OK 3 Select sub menu Setup Adjust Cell Constant press OK 4 A warning message will be displayed press OK 5 Enter the cell constant press OK The new...

Страница 28: ...can be set to off 1 Select main menu Setup press OK 2 Select menu Setup pH press OK 3 Select sub menu Setup Show pH press OK 4 Set the pH display on or off press OK B 2 6 Calibrating the temperature s...

Страница 29: ...ation the Monitor pH C 900 has on the UniNet bus It should correspond to the number set in UNICORN for the Monitor pH C 900 The number should be set to 0 if one pH C 900 is used If more than one pH C...

Страница 30: ...is due or the count down timer reaches 00 00 00 an alert display is shown and the instrument beeps until the OK button is pressed The display shows the time elapsed since the alarm and the current ti...

Страница 31: ...p Show Temp On Setup Conductivity Calibr pH Buffer 1 Calibr pH Buffer 2 Calibrated Electrode Slope 97 5 8 0mV Set pH Full Scale pH12 00 Set pH Zero Level pH2 00 Setup pH Temp Comp Off Setup Temperatur...

Страница 32: ...whichever is greater in the range 1 S cm to 300 mS cm Reproducibility short term Max 1 or 5 S cm long term Max 3 or 15 S cm Noise Max 0 5 of full scale calibrated range Response time Max 3 s 0 95 of...

Страница 33: ...hysical data Control Stand alone or from a PC with UNICORN version 2 20 or higher through UniNet 1 connection Degree of protection Housing IP 20 Flow cells IP 44 Power requirements 100 240 V AC 50 60...

Страница 34: ...as delivered from Amersham Pharmacia Biotech except for alterations described in the user manual used as stand alone unit or connected to other CE labelled Amersham Pharmacia Biotech instruments or ot...

Страница 35: ...1 8 o d 3 16 3 m 18 1112 47 Tubing connector for 3 16 o d tubing 10 18 1112 49 Ferrule for 3 16 tubing 10 18 1112 48 Stop plug 5 16 5 18 1112 50 Stop plug 1 16 5 18 1112 52 Union Luer female 1 16 mal...

Страница 36: ...18 1120 06_AC qxd 1999 04 14 14 16 Sida 32 1 1...

Страница 37: ...18 1120 06_AC qxd 1999 04 14 14 16 Sida 33 1 1...

Страница 38: ...April 1999 18 1120 06_AC qxd 1999 04 14 14 16 Sida 34 1 1...

Страница 39: ...18 1120 06_AC qxd 1999 04 14 14 16 Sida 35 1 1...

Страница 40: ...rear panel 2 The main operating menu RUN menu is shown 3 Calibrate the pH electrode before use and or daily by using 2 buffers with known pH values 4 The conductivity flow cell does not normally need...

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