Amersham Pharmacia Biotech AKTA design pH/C-900, Руководство пользователя

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5 Trouble shooting
5.1 General
When contacting Amersham Pharmacia Biotech for support, state
the program version of the instrument, which is shown for 2 seconds
during switch-on.
5.2 Faults and actions
If the suggested actions do not correct the fault, call Amersham
Pharmacia Biotech.
pH measurement (optional in ÄKTApurifier systems)
Fault Action
No response to pH changes 1 Check that the electrode cable is connected properly to
the rear of the instrument.
2 The electrode glass membrane may be cracked. Replace
the electrode.
Small response to pH changes 1 Clean the pH electrode according to section 4.3 and
recalibrate.
2 If the problem remains, replace the pH electrode.
1 Check the pH electrode tip. If it is contaminated
clean the electrode following the instructions in section
4.3 Cleaning the pH electrode.
2 If the membrane has dried out, the electrode may be
restored by soaking it in buffer overnight.
3 Clogged liquid junction. Refer to section 4.3 Cleaning the pH
electrode.
Incorrect/ unstable pH reading 1 Check that the electrode cable is connected properly to
the rear of the instrument.
2 Check that the pump and valves operates correctly.
3 Check that the electrode is correctly inserted in the flow cell
and, if necessary, hand-tighten the nut.
4 If air in the flow cell is suspected, tap the flow cell carefully
or tilt it to remove the air. Alternatively flush the flow cell with
buffer at 8 ml/min for 1/2 min.
5 Check that the pH electrode is not broken.
6 Check that the pH electrode is calibrated.
Monitor pH/C-900
User Manual 18-1120-06 Edition AC
15
Trouble shooting
5
Monitor pH/C900
V1.00
Slow pH response
or
Calibration impossible
WARNING! The instrument must not be opened by the user. It
contains high voltage circuits which can be capable of delivering a
lethal electric shock.
18-1120-06_AC.qxd 1999-04-14 14:16 Sida 15 (1,1)