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G4208A User’s Guide

109

3

Running an Isocratic Analysis

 

What You Will Need

What You Will Need

Instruments

Agilent Series isocratic, binary or quaternary pump, an autosampler and a 
UV- detector.

Column

A 125 mm × 4.0 mm Hypersil ODS, 5 

μ

m (Agilent Technologies part number 

7982618- 564).

Solvents

For the isocratic pump, a solvent mixture of LC grade bidistilled water (35 %) 
and acetonitrile (65 %).

Sample

The Agilent Technologies isocratic standard sample (Agilent Technologies 
part number 01080- 68704). This contains 0.15 wt.% dimethylphthalate, 
0.15 wt% diethylphthalate, 0.01 wt.% biphenyl and 0.03 wt.% o- terphenyl 
dissolved in methanol.

NOTE

The descriptions are based on Agilent 1100/1200 systems. The Agilent 1290 
Infinity System may have other requirements on the setup and material.

Содержание InfinityLab LC Series

Страница 1: ...Agilent Instant Pilot G4208 Agilent InfinityLab LC Series User s Guide G4208A User s Guide...

Страница 2: ...n this document that conflict with these terms the warranty terms in the separate agreement shall control Technology Licenses The hardware and or software described in this document are furnished unde...

Страница 3: ...general information around the Agilent Instant Pilot 2 Working with the Instant Pilot This chapter describes the operation of the Instant Pilot 3 Running an Isocratic Analysis This chapter describes h...

Страница 4: ...nt Pilot 20 Instant Pilot Display and Keyboard Layout 21 The i info key Online Information System 23 Basic Operational Concept of the Instant Pilot 26 Getting Started 27 System Information 33 Method I...

Страница 5: ...g an Isocratic Analysis 108 What You Will Need 109 Preparing the LC System 110 Entering Settings 111 Saving Settings in a Method 112 Creating a Sequence 113 Selecting a Signal 114 Observing the Chroma...

Страница 6: ...5 Firmware Updates 136 Troubleshooting 150 Repairing the Instant Pilot 152 Exchanging the CAN Cable 153 6 Appendix 157 Safety Information 158 Waste Electrical and Electronic Equipment WEEE Directive 1...

Страница 7: ...Pilot Display and Keyboard Layout 21 The i info key Online Information System 23 Basic Operational Concept of the Instant Pilot 26 Getting Started 27 System Information 33 Method Information 34 Sequen...

Страница 8: ...ted data Color display size 13 1 x 9 9 cm 5 0 x 3 8 inch 640 x 480 dots Install any desired configuration of Agilent 1100 1200 1260 1290 Series HPLC modules The Instant Pilot software will reflect whi...

Страница 9: ...select a variety of module tests that will check the performance of the LC system Use the early maintenance feedback EMF limits for scheduling maintenance work Display data graphically using the Plot...

Страница 10: ...configuration and state of system Flat dialog structure easy to understand icons Much faster confidence and usability less training required Automatic context sensitive help in status line Tool Tip Ea...

Страница 11: ...G4226A Autosampler G1316C TCC not compatible not compatible B 02 08 or above 1120 Compact LC not compatible not compatible not compatible Newer Series 1100 1200 modules that required special versions...

Страница 12: ...See Table 14 on page 152 CAUTION The operator of this instrument is advised that if the equipment is used in a manner not specified in this manual the protection provided by the equipment may be impa...

Страница 13: ...N Installation category II pollution degree 2 For indoor use only Research Use Only Not for use in Diagnostic Procedures NOTE This product with serial numbers DE and MY16355458 and below contains an T...

Страница 14: ...slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module Do not use an...

Страница 15: ...asy to use safe placement of the Instant Pilot stable mechanism easy upgrade of all Instant Pilot s with old holder version possible see Parts Identification on page 152 Figure 2 on page 15 shows all...

Страница 16: ...tors into the CAN or vice versa since the CAN uses 24 V and might damage the LAN card CAUTION The CAN bus does not support hot plugging or unplugging Unwanted or unexpected behavior of the instrument...

Страница 17: ...apter plate delivered with the Instant Pilot from the front onto the top cover of the Agilent HPLC module 2 Assure that it is fixed by pressing onto the adapter plate 3 Insert the Instant Pilot into t...

Страница 18: ...inity II Kit The kit 5067 5955 part of the Instant Pilot G4208A from May 2015 includes Holder Infinity II Screw Installation Note Procedure Refer to the User Manual of your Infinity II module 1 Remove...

Страница 19: ...stant Pilot to an Agilent HPLC Module 5 Fix the holder with the screw 6 Re install the Leak Interface Top and the door 7 Insert the Instant Pilot into the holder 8 Connect the CAN cable of the Instant...

Страница 20: ...he Instant Pilot slightly angle the Instant Pilot 1 and then lift it from the holder 2 Figure 3 Removing the Instant Pilot CAUTION The CAN bus does not support hot plugging or unplugging Unwanted or u...

Страница 21: ...out Figure 4 on page 21 shows the layout of the display and the keys All has been arranged in functional groups around the display Figure 4 The Agilent Series Instant Pilot Layout 1 2 3 4 5 6 7 8 9 NO...

Страница 22: ...the last window or screen you were working with In an edit field the previous value can be restored by pressing the Esc key OK key or 8 you accept a current entry or action When entering a parameter i...

Страница 23: ...ine information system is context sensitive and provides information related to the current topic You can access the online information system by using the i info key on the Instant Pilot s keyboard F...

Страница 24: ...08A User s Guide 24 1 Start up Information The i info key Online Information System Figure 6 Online Information System Content left Home right Figure 7 Online Information System Index left Details rig...

Страница 25: ...d link Esc exits the online help or moves the curser to previous or next link or moves the curser up or down to a link Content shows the content as book structure How Tos Reference Concepts Error Mess...

Страница 26: ...n the analysis result Chemists change these parameters to set up optimal conditions for a certain analysis Control Button All control menu items directly trigger a day to day action on the instrument...

Страница 27: ...en run red error In the bottom right the current time and the USB Flash Drive icon is shown If a USB Flash Drive is connected the icon is shown as active blue NOTE If additional details are required o...

Страница 28: ...e Time Units Formats and the Display Use the Direction keys for moving to the fields and Edit or OK to open the selection fields 3 Select Next to set date and time Figure 8 Getting Started Setup Wizar...

Страница 29: ...1 Start up Information Getting Started 4 Select Next to enter a system name Figure 9 Getting Started Setting a System Name A system name will be displayed on screens and printouts as identifier Syste...

Страница 30: ...d formats Figure 10 Getting Started Setup Wizard Units Formats 6 Select Next to define display settings Figure 11 Getting Started Setup Wizard Display 24 12 h Month Day Year Day Month Year Bar PSI kPa...

Страница 31: ...Started Setup Wizard LAN settings 8 Select Modify to change LAN settings The next screen shows the actual LAN settings used for communication with the system module Figure 13 Getting Started Setup Wiz...

Страница 32: ...e Configuration screen is displayed Figure 14 Getting Started Welcome Screen The next time the Instant Pilot is started it will start with the Welcome screen To activate the setup wizard again select...

Страница 33: ...ime and Board ID of the modules Some modules may show additional information e g the G1316A Column Compartment shows an installed column switching valve or a module with installed LAN interface card s...

Страница 34: ...ion about module settings and parameters of all modules Depending on the number of modules you may have to scroll through the display 2 To change a parameter scroll down to the field and press Edit or...

Страница 35: ...timetable screen and is stored together with the method You can edit lines copy paste lines delete lines and insert new lines You can choose if the list should be ordered by module default or time Pro...

Страница 36: ...be processed from top to bottom 2 Use the Insert button to insert items in the list OR In case of samples and calibration samples use the Wizard to insert items in the list OR Use the Edit Delete or...

Страница 37: ...tus graphically Properties The properties of a sequence can be reviewed in the Properties screen You can view changes and the reasons for them and change also the protection of he current sequence See...

Страница 38: ...ile itself can also hold up to four smaller tiles The Instant Pilot automatically chooses the size of the tiles based on the selection The display shows the actual connected and powered up Agilent mod...

Страница 39: ...automatically be rescaled for best on screen fitting Setup lets you set up the views Select one of the last 4 setups can be loaded Control opens a menu to control certain sys tem activities for detail...

Страница 40: ...s entry in the list or if it is an empty tile the last selected entry In the selection list the tile types have the same naming as in the setup dialog editable to have a large or small tile editable...

Страница 41: ...list select a signal parameter and press Select The selection will be taken for the selected window Figure 20 on page 41 shows the relation of the windows in the Setup screen versus displayed windows...

Страница 42: ...history of the current status view changes and the protection of the status view Figure 21 Status Screen Properties History Figure 22 Status Screen Protection protect unprotect a status view with a p...

Страница 43: ...n system setting activities 4 To leave the screen press Exit or Esc 5 Press Print The logbook is saved to a connected USB Flash Drive The text is written and saved into folder PRINTOUT as LOGBOOK MHT...

Страница 44: ...up Information Logbook Information Figure 24 Logbook Screen saved to USB Flash Drive Table 9 Legend of Logbook Icons Entries Icon Event status change event Info event error event EMF Early Maintenance...

Страница 45: ...on move to the line you want to change and press Edit After doing the changing press OK or Done The Instrument Name will appear as identifier on the screens e g Welcome or printouts reports 3 To start...

Страница 46: ...firmware must be on an inserted and activated USB Flash Drive in its root directory On the module specific screens you can see the Early Maintenance Feedback EMF error and maintenance events set the E...

Страница 47: ...ss the Exit button or Esc key to leave the screen Figure 27 Maintenance Screen Pump Figure 28 Maintenance Screen Select Maintenance Activity EMF setup Identify module LED blinks select maintenance act...

Страница 48: ...he EMF limits and the limit has been reached a message pops up Figure 29 Early Maintenance Feedback EMF Message 1 The limits can be set in the EMF Setup screen Figure 30 Early Maintenance Feedback EMF...

Страница 49: ...w board does not have a serial number For some modules e g pumps or auto samplers the type has to be changed multiple usage boards Use the information from the serial number plate of your module The c...

Страница 50: ...reen shows a list of all modules in the system with their available tests 2 To select a test scroll down to the list and press Exec or OK to start the test A test report is shown at the end of the tes...

Страница 51: ...s on off state The modules are grouped by modules types Pumps TCC temperatures and Detectors lamps with a frame next to the buttons on the right side The correlation to the buttons on the right side g...

Страница 52: ...dule has been enhanced It allows to set up a simple analysis by pressing the START key adding the vial range and number of injections selecting the current or a different method internal or from USB F...

Страница 53: ...screen Sequence Analysis screen Vial range Start button Sample Range Analysis screen ON OFF on various screens Control button System ON OFF and System Get Ready on various screens System screen Welcom...

Страница 54: ...rol Module to Instant Pilot Status screen Welcome screen Status Plot screen Welcome screen Status Plot Spectrum DAD MWD VWD FLD Control button on various screens Table 10 G1323A B Control Module vs In...

Страница 55: ...are It is always backward compatible with all previously released versions A 05 15 or later for firmware platform A 05xx Update main part only B 02 17 or later for firmware platform A 06 xx B 01 xx or...

Страница 56: ...ram 79 Sequence Automating Analyses 84 Using the Sequence Wizard 86 Saving a Sequence 88 Sequence File Protection 90 Tray View 91 Starting and Stopping a Sequence 92 Displaying Data Graphically 95 Set...

Страница 57: ...ting a USB Flash Drive The display shows whether the USB Flash Drive is inserted and active by an icon not present grey present blue active yellow with red dot do not unplug NOTE Since USB Flash Drive...

Страница 58: ...upported USB Flash Drive 1 When selecting No theUSB Flash Drive will be ignored can not be used in the Instant Pilot even it is still inserted OR When selecting Yes there are two possible responses fo...

Страница 59: ...rive into a folder PRINTOUT The files are of type MHT or HTM depending on the setting in Configuration Controller Print document as The difference is The files can be opened with a PC using Microsoft...

Страница 60: ...G4208A User s Guide 60 2 Working with the Instant Pilot Printing To USB Flash Drive Figure 37 Example of a Printed Document Instrument Logbook The following information can be printed...

Страница 61: ...DETSCAN MHT sample scan VWD Scan Print DETSCAN MHT blank reference and sample scan FLD Scan Print DETSCAN MHT Excitation or Emission scan Calibrations via More button and Maintenance DAD Calibration...

Страница 62: ...Nano Pump Prep Pump Print DIAGRES MHT Preparation is described in Help Preparation steps like purging the pump are NOT included in the automatic actions list itself Table 11 Overview of Printable Inf...

Страница 63: ...Guide 63 2 Working with the Instant Pilot Printing To USB Flash Drive Figure 38 Example of a Printed Document DAD Holmium Test system name date name of test plot module serial number plot actions res...

Страница 64: ...Flash Drive method The method parameters are stored on a USB Flash Drive A method that is stored on the USB Flash Drive can be loaded to the LC modules or transferred to another LC system Methods can...

Страница 65: ...t a method from the list 6 Press OK or Load The Method Module screen lists all methods that are stored in the modules For each method there is a date when the method was last changed When a method is...

Страница 66: ...t screen If you change a method setting the value is immediately downloaded to the LC module An asterisk will appear next to the method name to indicate that the current method has been modified An ha...

Страница 67: ...ctory settings OR Using File the filter settings can be stored and or stored filter setting can be loaded If a filter is set the Method screen will show the information Method name filtered The filter...

Страница 68: ...43 Method Compare screen If there are differences in the configuration and or timetable a message is shown in the status line and you can view the differences via Config Displayed Method is the actua...

Страница 69: ...ngth In other cases e g solvent composition these changes take place dynamically approaching the set value in a stepwise and linear manner The Timetable screen shows the timetable used in the currentl...

Страница 70: ...nd the desired parameter change Setting Select the parameter to be changed Value Enter the desired parameter value You can edit an existing timetable line by pressing Edit or OK Use Delete to delete t...

Страница 71: ...method can be protected unprotected with a password Figure 46 Method Properties screen The method can be protected against inadvertent changes Any change to the method is not accepted until the method...

Страница 72: ...is allowed and will be stored under the new name unprotected Using the same name results in File Save Failed Permission denied error Renaming a protected file is not allowed Transfer of protected file...

Страница 73: ...with the Instant Pilot Working with Methods A password to protect a file can have up to 12 digits If left empty no empty password will be added to the file protection Figure 47 Method Protection Figu...

Страница 74: ...d depends on the number of timetable and injector program lines included In general more than 100 methods may be stored in the Instant Pilot With differing method contents the actual amount of methods...

Страница 75: ...her 4 You may protect un protect a method see Method Properties on page 71 and Method File Protection on page 72 The stored method now contains all the current LC system and module settings If you dis...

Страница 76: ...File Transfer dialog allows you to transfer files between internal file storage and the connected USB Flash Drive Figure 51 Method Transfer 1 Select a method 2 Press Transfer The method is transferred...

Страница 77: ...to the modules The offline method dialog starts with a copy of the actual method The offline mode is emphasized by the different dialog color Figure 52 Method Save As All buttons have the same functio...

Страница 78: ...or on the USB Flash Drive Export is not possible Figure 53 Method Import G1323 methods from USB generated with G1323 Control Module then transferred via G1323 PC card PC USB Flash Drive to the Instan...

Страница 79: ...5 11 November 2006 the Injector Program has been implemented The injector program is part of the method The injector program screen can be accessed by pressing edit on the Injection Mode line and chan...

Страница 80: ...modified or expanded Figure 54 Injector Program Default Program 2 Move to a line of the Injector Program and press Edit button to view the current settings or start a new line Figure 55 Injector Prog...

Страница 81: ...56 Injector Program Setup Screen 4 Move to line End of User Defined Injector Program press the Insert button and select additional action items as required Via the Edit button you can change the param...

Страница 82: ...eat Eject x l into sample Eject x l into location y Eject x l into seat Eject x l into air Mix x l in seat z time s Mix x l in air z time s Mix x l in air at y l min at z time s Mix w l in location x...

Страница 83: ...ample position x vial s ALS only Increment actual sample position w tray s x plate s y row s z column s WPS only Reset actual sample position ALS only Reset actual tray position WPS only Reset actual...

Страница 84: ...hod to analyze further samples with different analytical conditions When the second method is loaded it waits for a specified time before starting the analysis allowing the column to equilibrate to th...

Страница 85: ...using the Calibration Settings screen The Calibration Settings screen is accessed by selecting Calibration in the Sequence Wizard screen You can re calibrate using one or more standards and have the f...

Страница 86: ...lyses Using the Sequence Wizard You may use the Wizard to set up a sequence Figure 60 Sequence Wizard Adding Samples Information Figure 61 Sequence Wizard Adding Calibration Information See Figure 62...

Страница 87: ...s Guide 87 2 Working with the Instant Pilot Sequence Automating Analyses Figure 62 Sequence Wizard Preview with Calibration Parameter Multi Figure 63 Sequence Wizard Preview with Calibration Paramete...

Страница 88: ...e stored depends on the number of timetable and injector program lines included In general more than 100 squinches may be stored in the Instant Pilot With differing sequence contents the actual amount...

Страница 89: ...as 2 Choose the location internal Instant Pilot or USB USB Flash Drive and a name if not already done Figure 65 Sequence Save As 3 You may delete or copy sequences from one location to the other Delet...

Страница 90: ...y protect un protect a sequence see Method Properties on page 71 and Method File Protection on page 72 Differences are Edit Insert Delete Copy Wizard and Save buttons are disabled There is no Import f...

Страница 91: ...senting their states The current sample is colored alternating blue sample or magenta calibration sample and light blue The currently processed sample location and the method name are also shown textu...

Страница 92: ...ted line If no sequence is currently paused this functions is disabled The sample range possibility is for running sequences without parameter changes for the specified method s Figure 67 Sequence Sta...

Страница 93: ...uired lamps are turned on are performed automatically Figure 68 Sequence Get Ready screen When all Get Ready activities are complete the system status changes to green the sequence starts immediately...

Страница 94: ...ss Stop the Stop Analysis dialog pops up where you can select between aborting immediately or pausing the sequence Continue aborts or pauses depending on the selection the current sequence A shortcut...

Страница 95: ...are user selectable can automatically be rescaled for best on screen fitting Figure 71 Plot screen The Print button allows the print of the plot window to the USB Flash Drive Use the Setup button to...

Страница 96: ...s Figure 72 Plot screen Setup signals On the right side of the Selected Signals list box you can see the legend to the signals The different signals can be selected by pressing Toggle or OK Depending...

Страница 97: ...the Y signal unit axis Via Setup you can specify a Y range separately for each signal Rescaling directly from the Plot screen overwrites these settings Use Rescale in the Plot screen to adjust the Y a...

Страница 98: ...trum Using the Start Stop button starts stops a continues spectrum Using the Cursor buttons the cursor can be moved left or right on the wavelength axis Using the Balance button the detector performs...

Страница 99: ...ctrum and Emission Spectrum Both screens are similar only the editable parameters are different according the selected spectra type see Figure 75 on page 99 The screen has a Single scan mode and a Con...

Страница 100: ...ant Pilot DAD MWD VWD FLD Spectrum Warnings are shown if the method parameters are not set to produce spectra information and if the lamp in not switched on Start keys will be kept disabled until vali...

Страница 101: ...ector included in all modules in order to synchronize the analyses This is necessary when an external device needs some time to get ready for a new analysis and thus the transmission of a start reques...

Страница 102: ...nalysis the injection needle is placed in the seat and the valve is in the proper position are fulfilled a Start signal is generated to inform the other modules that now the analytical run starts Star...

Страница 103: ...board LAN and your system is integrated in a LAN USB This interface has been introduced with the Infinity II Fusion modules and replaced the RS 232 interface The USB might be used for the Agilent Lab...

Страница 104: ...tion must be enabled protocol mode only If the Instant Pilot starts an analysis the Agilent ChemStation is the slave monitor system The Agilent or 3rd party user interface may block certain functional...

Страница 105: ...rive with Method Save As If the method is on the Instant Pilot or USB Flash Drive and should be saved on the Agilent ChemStation first load method DEF_LC M on the Agilent ChemStation to have no additi...

Страница 106: ...ntrollers To change the setting open the Configure Controller screen Scroll to 3rd Party Software and change to ON Figure 78 Configure Controller 3rd Party Software Compatibility Backward compatible O...

Страница 107: ...reenshot for adding it to a documentation or troubleshooting reasons To do so 1 Insert the USB Flash Drive 2 Wait until the USB Flash Drive has been initiated 3 Press the key sequence i dot info The s...

Страница 108: ...eparing the LC System 110 Entering Settings 111 Saving Settings in a Method 112 Creating a Sequence 113 Selecting a Signal 114 Observing the Chromatogram 115 This chapter describes how to analyze the...

Страница 109: ...vents For the isocratic pump a solvent mixture of LC grade bidistilled water 35 and acetonitrile 65 Sample The Agilent Technologies isocratic standard sample Agilent Technologies part number 01080 687...

Страница 110: ...itrile channel B 2 Turn on the detector lamp and pump via the Control System On OFF screen 3 For the quaternary pump turn on the degasser by pressing the line power switch 4 Purge the pump 5 Allow the...

Страница 111: ...hen save these settings to a method called ISO 1 Enter the Method screen 2 Select Control and select System Set Defaults 3 Set the Stoptime to 6 min 4 Set the Flow to 1 5 mL min 5 Set B 65 C OFF D OFF...

Страница 112: ...n a Method Saving Settings in a Method 1 Select File in the Method Information screen 2 Select Save As 3 Enter the method name as ISO using the selection keys also see Method File Protection on page 7...

Страница 113: ...thod named ISO and press OK 4 Press Insert and select Sample If your sample is not in vial 1 you have to modify the vial number also see Sequence Automating Analyses on page 84 5 Select File and selec...

Страница 114: ...n 2 Select Status in the Welcome screen 3 Press Plot 4 Press Setup 5 Choose a Detector Signal from the Available Signals list 6 Press Done NOTE You can choose several signals at a time The plot functi...

Страница 115: ...lot to show the chromatogram A typical chromatogram for this analysis is shown in Figure 79 on page 115 Figure 79 Analysis of Isocratic Standard Sample The exact profile of the chromatogram depends on...

Страница 116: ...h the Same Group of Standards 123 Re calibrating With Multiple Groups of Standards 126 Synchronizing Analyses with External Devices 129 Standard Mode 130 Send Single Start Request 130 Send Multiple St...

Страница 117: ...on describes how to set up a 25 vial analysis with one injection from each vial You will use a previously created method The samples are located in positions 1 to 25 of the autosampler tray For detail...

Страница 118: ...h two injections per vial the last 10 vials are analyzed using METH3 with three injections per vial The vials are located in positions 1 to 50 of the autosampler tray The example assumes that the sequ...

Страница 119: ...t 11 Select Method and then move to METH3 and press Load 12 Move to the End of sequence list and select Insert 13 Select Wait time and enter 14 Select Done to accept entries 15 Move to the End of Sequ...

Страница 120: ...after every 2 samples C SS C SS C SS C SS C S C the calibration standard is located in position 90 and the 9 sample vials are in positions 1 to 9 of the autosampler tray and the method called METH1 i...

Страница 121: ...G4208A User s Guide 121 4 Running Multiple Vial Analyses Single Level Calibration Sequences 6 Change the settings according to Figure 80 on page 121 Figure 80 Sequence Calibration Wizard...

Страница 122: ...nning Multiple Vial Analyses Single Level Calibration Sequences 7 Press Preview to view the result Figure 81 Sequence Calibration Wizard Preview 8 Select Done to accept entries 9 Press Start and confi...

Страница 123: ...e the samples and re analyzed twice after every 5 samples C1 C2 C3 S10 S14 C1 C2 C3 S15 S19 C1 C2 C3 S20 S24 C1 C2 C3 the calibration standards are located in positions 90 92 of the autosampler tray t...

Страница 124: ...ultiple Vial Analyses Multiple Level Calibration Sequences 6 Change the settings according to Figure 82 on page 124 Figure 82 Sequence Calibration Wizard 7 Press Preview to view the result Figure 83 S...

Страница 125: ...art and confirm the start of the sequence The autosampler now analyzes the three calibration standards in duplicate sample vials 10 through 14 the three calibration standards in duplicate sample vials...

Страница 126: ...ions 7 to 12 and the samples and the calibration standards use the same method called METH1 For sample type B there are 3 calibration standards of different concentrations and 9 samples each sample mu...

Страница 127: ...n Wizard 7 You may press Preview to view the result 8 Select Done to accept entries 9 Move to the End of sequence list and press Insert 10 Select Method and then move to METH2 and press Load 11 Move t...

Страница 128: ...ration standards in duplicate type A samples in vials 7 and 8 three type A calibration standards in duplicate type A samples in vials 9 and 10 three type A calibration standards in duplicate type A sa...

Страница 129: ...necting External Devices on page 101 for further information on interfaces The synchronization mode can be chosen in the Configure System screen Figure 86 Choosing the Synchronization mode In any case...

Страница 130: ...mmand at the point of injection With an Agilent Autosampler integrated in the system and no external devices this is the normal mode of operation Send Single Start Request After you start the analysis...

Страница 131: ...he external device starts each injection then by sending a start signal to the APG remote line That is after all the programming has been completed on the Instant Pilot and the Start button has been p...

Страница 132: ...fter pressing the Start button the Instant Pilot waits for a single external start request on the APG remote lines When the start request is received the complete vial range or sequence is done as in...

Страница 133: ...before injection or sequence The start requests have to be generated by the external device This mode is recommended when a device needs extra time to get ready for the next analysis and thus has to...

Страница 134: ...l perform a balance assumed that Auto Balance is set to pre run when receiving a start command from the Instant Pilot This will only happen in the Standard and Wait for single multiple start request m...

Страница 135: ...lent Lab Advisor 143 Using the LAN RS 232 Firmware Update Tool 145 Errors During Firmware Updates 149 Troubleshooting 150 Troubleshooting the Instant Pilot 150 USB Flash Drive not recognized 151 Insta...

Страница 136: ...the firmware of the main system Main System It s poperties are the complete communication capabilities CAN LAN USB and RS 232C on the Instant Pilot only CAN memory management ability to update the fir...

Страница 137: ...Update Tools Update via Module Instant Pilot G4208A LAN RS 232 Update Tool 2 10 and a PC with LAN or RS 232 Lab Advisor B 02 06 and above and a PC with LAN RS 232 USB Instant Pilot G4208A USB Flash D...

Страница 138: ...module you want to update NOTE Only one firmware revision set should be stored on the USB Flash Drive If more than one firmware version for a module is available the Instant Pilot always takes the mo...

Страница 139: ...eady for Update 4 Press Cont button to confirm and Cont start the update or Cancel to exit this screen The actions and the progress is shown Figure 93 Firmware Update Screen Update has completed In th...

Страница 140: ...mware revision versus the available 2 Press Update Wizard and then Cont or Abort to cancel the update process NOTE Only one firmware revision set should be stored on the USB Flash Drive If more than o...

Страница 141: ...revisions on the USB Flash Drive Figure 95 Firmware Update Screen Update Wizard 4 Do your selections and press Update or Cancel to exit the screen Figure 96 Firmware Update Screen Update in Progress...

Страница 142: ...firmware update Upgrade to Revision B xx xx When the Instant Pilot needs to be upgraded to a B xx xx firmware to be compatible with Agilent 1100 1200 series modules working on A 06 xx follow the proc...

Страница 143: ...Firmware Updates Using the Agilent Lab Advisor 1 Start The Lab Advisor 2 Connect to the HPLC system The modules will be listed Figure 97 Lab Advisor System Overview 3 Select Firmware Update Then lock...

Страница 144: ...ct the firmware version for the Instant Pilot Main and Resident and or the other modules Use always the latest version 2 Start the Update 3 Figure 99 Lab Advisor Select the firmware 5 The firmware wil...

Страница 145: ...ntrol Software on page 106 it will not be seen by the Firmware Update tool For updating firmware reconfigure the Instant Pilot to Backward compatible mode OFF NOTE If your Agilent 1100 1200 system run...

Страница 146: ...te Tool 2 10 indicates 100 updated but does not display the result dialog 3 Press Cancel red cross button on the LAN RS 232 Update Tool 2 10 to stop the Update process The Instant Pilot is no longer l...

Страница 147: ...ter boot enter the Maintenance screen and press the Controller button Figure 101 Update Information for firmware A 05 11 4 Press the Transfer button The Instant Pilot will switch into resident mode bl...

Страница 148: ...Update Tool lists now the Instant Pilot 5 Select the Instant Pilot for update to the target firmware revision 6 Start the update 7 After boot of the Instant Pilot the Instant Pilot shows Scanning Syst...

Страница 149: ...ing Firmware Updates If an error stops the update process it is displayed Figure 102 Firmware Update Screen Error during the update Press Abort to leave the Update process In the above example all mod...

Страница 150: ...are listed even duplicates up to 1000 entries This logbook can be saved to the USB Flash Drive Screenshots also can be saved to the USB Flash Drive see Saving a Screenshot to USB Flash Drive on page...

Страница 151: ...vendor or from type to type incompatibilities can occur In general USB Flash Drives from Sandisk and Kingston should work The USB Flash Drive must be FAT 16 formatted and without encryption See Handl...

Страница 152: ...Kit Infinity II for adapting the Instant Pilot to Agilent 1200 Infinity II series modules see Holder Versions for the Instant Pilot on page 15 Includes holder and screw 5067 5955 USB Flash Drive Kit...

Страница 153: ...across the screws CAUTION Voltage in the Instant Pilot Damage to the electronics Before you open the Instant Pilot the CAN cable must be disconnected from the HPLC module to assure that no voltages ar...

Страница 154: ...G4208A User s Guide 154 5 Maintenance and Repair Exchanging the CAN Cable 3 Remove the six screws that fix the rear panel Figure 103 Rear Panel location of screws 4 Remove the rear panel carefully...

Страница 155: ...able into the connector 2 8 Fit the cable with the cable relief 3 in the front cover 9 Place the release button into its location 1 NOTE Step 5 and 9 are for the OLD rear panel design only See informa...

Страница 156: ...rear panel and fit the release button pin into the recess on one end of the connecting rod of the rear panel see Figure 105 on page 156 Figure 105 Pin of release button fits into the recess of the ro...

Страница 157: ...158 Safety Symbols 158 General 159 Operation 159 Waste Electrical and Electronic Equipment WEEE Directive 160 Radio Interference 161 Test and Measurement 161 Agilent Technologies on Internet 162 This...

Страница 158: ...ply with these requirements Safety Symbols Table 15 Symbols The apparatus is marked with this symbol when the user shall refer to the instruction manual in order to protect risk of harm to the operato...

Страница 159: ...d Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate th...

Страница 160: ...product is classed as a Monitoring and Control instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent c...

Страница 161: ...to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measur...

Страница 162: ...s Guide 162 6 Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www a...

Страница 163: ...010 Part No G4208 90010 Rev B Document No SD 29000124 Rev B In This Guide This book provides information about the Agilent Instant Pilot The manual describes the following Start up Information Working...

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