Agilent Technologies Infinity Binary Pump VL 1290, Руководство пользователя

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50 Agilent 1290 Infinity Binary Pump User Manual
4
Using the Pump
Setting up the Pump with the Instrument Control Interface
Method Parameter Settings
The Binary Pump method setup parameters are in eight sections:
• Flow
• Solvents A and B
• Stoptime
• Posttime
• Pressure Limits
• Timetable
• Advanced
• External Contacts
Table 5 Method parameters
Parameter Limits Description
Flow 0.00 – 5.00 mL/min
in steps of 0.001 .
Recommended flow
range: 0.05 –
5.00 mL/min . The flow is the rate of movement of eluent along the column. It is
important that the flow rate is kept constant to ensure precise retention
time, and peak measurements. Variations in flow rate can occur as a result
of the partial failure of the pumping system, air in the pumping system, a
change in the mobile phase viscosity or a temperature change.
Solvents A and B For each channel, you can select which of the two solvents to deliver. You
can set the percentage of solvent B to any value from 0 through 100 % .
Solvent A always delivers the remaining volume: 100 - %B. The solvent B
check boxes allow you to turn the solvent B channels on (checked) or off
(cleared).
When the
Use solvent types check box in the Compressibility section is
checked (see “Advanced Settings” on page 52), you click the down arrow
and select either a Generic solvent or a calibrated Solvent .
Stoptime 0.01 – 99999 min or
As Injector/No Limit
(an infinite run time).
The stoptime sets a time limit for your analysis. After the stoptime, all
gradients are stopped and the pump parameters return to their initial
values. The pump can be used as a stoptime master for the complete
analytical system. The pump also stops the detectors if they have a
No
Limit stoptime setting. If no limit is given, a method will have to be stopped
manually.
Posttime 0.01 – 99999 min or
Off (0.0 min ).
Your instrument remains in a not ready state during the posttime to delay
the start of the next analysis. You can use the
Posttime to allow your
column to equilibrate after changes in solvent composition (for example
after gradient elution).