3019 FLUORESCENCE MICROSCOPE SYSTEM
ACCU-SCOPE
®
73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com
14
FLUORESCENCE OPERATION
Preparation
Verify that the voltage and the frequency of the AC
main outlet match the setting of the voltage switch
and the frequency switch on the rear of the power
supply unit.
Make sure the cord is connected firmly.
When transmitted light observation is required, pull
out the filter system and rotate the blank opening
into the light path.
Adjust the field diaphragm to match the field edge.
If it is not centered, use the Allen wrench to adjust
the screw.
When it is required to interrupt observation for a
short period, use the shutter mechanism on the
fluorescence illuminator to block the light
accessorial excitation filter part. (Repeated on-off
of the mercury lamp will shorten its service life
considerably).
Precautions on the specimen color fading:
The system employs high-intensity excitation light
to enable bright observation of dark fluorescence
specimens. As a result, if high-power objectives
are used frequently, color fading of the specimen
occurs early, degrading the view (contrast) of
fluorescence images. So it is effective to use the
shutter frequently to avoid illuminating the
specimen for a longer period than required.
A ND (neutral density) filter and small aperture
diaphragm can help weaken the intensity of the
excitation light. Also, it is useful to use the light
shutter to reduce the specimen color fading.
Color fading of the specimen can also be delayed
using commercially available color fading
preventing agent (DABCO, etc). The use of color
fading preventing agent is recommended when
you perform high-magnification observation
frequently.
NOTE:
color fading preventing agent cannot be
used with certain specimens
