3019 FLUORESCENCE MICROSCOPE SYSTEM
ACCU-SCOPE
®
73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com
16
FLUORESCENCE OPERATION
(continued)
Centering the Mercury Bulb (Fig. 20-22)
Rotate the nosepiece to the 10x objective
④.
Slide the fluorescence filter selection lever
③
to move the
fluorescence filter cube B1 into the light path.
Slide the field diaphragm adjustment lever
⑤
(Fig. 21) to the
top position. This opens the field diaphragm to its maximum
position.
Adjust the condenser focusing knob
⑥
(Fig. 21), the vertical
adjusting knob
⑦
of mercury bulb, and the horizontal
adjusting knob
⑧
of mercury bulb to make the bulb image on
the “+” scale of the slide
①
. (Fig. 22 A)
Adjust the knob
⑪
of the parabolic mirror to focus, the
horizontal centering knob
⑨
, and the vertical centering knob
⑩
to make the bulb mirror
image on the “+” scale of the slide
①
. (Fig. 22 B)
Adjust the knob
⑨
and
⑩
to make the bulb image and bulb
mirror image symmetrical
to the “+” scale of the centering
slide
①
. Adjust the knob
⑪
to make both images the same
size. (Fig. 22 C)
Adjust the screw
⑦
and
⑧
to make the two images
superpose on
the center of “+” scale. (Fig. 22 D)
Centering the bulb after it warmed up will be more
precise.
After replacing the mercury bulb (usually 200 hours), it
should be re-centered.
Fig. 20
Fig. 21
Fig. 22
