3019 FLUORESCENCE MICROSCOPE SYSTEM
ACCU-SCOPE
®
73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com
13
ADJUSTMENT & OPERATION
(continued)
Using the Oil Objective (100x)
The procedure for examining a specimen using an
oil immersion objective is as follows:
Rotate the nosepiece so the low power objective is
in the optical path.
Place one drop of immersion oil on the lighted area
of the specimen slide
①
(Fig 17). Dust or air
bubbles in the oil can destroy the definition of the
image. If the bubbles are trapped between the
objective lens and the slide, clean off the oil and
start again or try to eliminate the bubble by rotating
the objective back and forth.
Rotate the nosepiece so the 100xR oil immersion
objective is in the light path.
With your eye at the level of the stage, use the
coarse focus knob to raise the stage with the
specimen cover glass. When you see a flash of
light at this location the objective lens has made
contact with the immersion oil and the microscope
can now be focused using the fine focus knob.
Each time you finish using the oil immersion
objective wipe off all traces of oil from the objective
and the specimen cover glass with a lens tissue or
clean soft cloth. Cleaning after each use will
prevent oil from contaminating the high dry
objective (40xR) and deforming its optical
performance, prevent dust and dirt from
accumulating on the lens of the objective and
degrading its optical performance, and will keep the
slide clean to work with.
Fig. 17
①
