3019 FLUORESCENCE MICROSCOPE SYSTEM
ACCU-SCOPE
®
73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com
17
FLUORESCENCE OPERATION
(continued)
Centering the Field Iris Diaphragm (Fig. 23-25)
Switch the light shutter to “●” closed position.
Turn filter block turret to engage the B-excitation mirror in the
light path.
Switch the light shutter
①
to “O” open position.
Rotate the 10× objective in the light path, and place the
specimen on the stage and bring into approximate focus.
Pull the field iris diaphragm lever out until the diaphragm
comes into the smallest state.
Use the two centering screws
②
to adjust the two field iris
diaphragm centering screws alternately to move the image of
the diaphragm to the center. Fig. 24 shows the adjustment of
diaphragm.
Move the field diaphragm lever to open the diaphragm. As
this makes slight deviation noticeable, adjust the centering
precisely.
Enlarge the diaphragm until it just circumscribes the field of
view.
Select the Fluorescence Filter Cube (Fig. 25)
The filter block should be accordance with the specimen.
Pull the filter flashboard
①
to the most right position.
(See Fig. 25)
Use the filter selecting lever
②
to select the needed filter
cube.
Fig. 23
Fig. 24
Fig. 25
