TMA Grand Master 2.6 – User’s Guide
5 Using TMA Grand Master
Important!
Each donor position has its adherent PCR position (for example, A6–PCR6) and because
of the maximum number of PCR markers allowed to place on a donor block is four,
each PCR tube can contain up to four cores of the corresponding donor block. A PCR
cassette (when loaded with 10 PCR tubes) is capable of receiving 40 cores total.
Important!
It is available for you to import markers already placed on the virtual slide.
Important!
PCR process is not supported in case the software was restarted after exiting without
closing it properly or loading the project that has been saved previously.
2. If you want to remove an existing marker, click it with the right mouse button. The
corresponding layout position of the deleted marker will therefore be free for the next
defined marker.
3. Core transfer from the donor block(s) to the recipient block or PCR tubes starts after clicking
the
Start
button below the image of the donor block.
If you have not entered Recipient block ID, nor Donor block ID for the selected transfer, a
window opens in which you must enter the Ids.
TMA Grand Master
inserts each core into the next available spot, and proceeds from left-to-
right and from top-to-down order in the layout grid of the TMA block.
If you have activated the new view for donor blocks, the block on which you have selected a
core to be transferred and clicked
Start
, the thumbnail of that specific block will be moved to
the back end of the block queue.
December 9, 2016 – Rev. 1
3DHISTECH Ltd.
51(92)
