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TMA Grand Master 2.6 – User’s Guide

4 Operating the Main Unit

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December 9, 2016 – Rev. 1

3DHISTECH Ltd.

27(92)

Содержание TMA Grand Master 2.6

Страница 1: ...GM26 UG EN Rev1 TMA Grand Master 2 6 User s Guide December 9 2016 Rev 1 ...

Страница 2: ...ating the Main Unit 21 4 1 Switching the Main Unit On or Off 21 4 2 Starting the TMA Control Application 21 4 3 Main User Interface 24 4 3 1 Main Toolbar 25 4 4 Inserting and Removing Blocks 28 4 4 1 Block preparation 28 4 4 2 Cleaning block and PCR cassette preparation 29 4 4 3 Inserting a block into the main unit 30 4 4 4 Removing a block from the main unit 32 4 4 5 Removing a PCR cassette from ...

Страница 3: ...6 1 Preventive Maintenance of the Main Unit 79 6 2 Cleaning Tasks 79 6 2 1 Opening the Service Compartment Door 80 6 3 Replacing fuses of the main power switch 81 6 4 Contaminated Tray or Touch Sensor Surface 82 7 Troubleshooting TMA Grand Master 84 7 1 Hardware related issues 84 7 2 Software related issues 85 8 Cassette and Block Dimensions 88 9 Technical Description of the Hardware 89 9 1 Physic...

Страница 4: ... analysis of the digitized slides for example change of health status related to erroneous diagnosis from the digitized slide s that stems from or is associated with use of Product digitized slides quality of staining quality of stained slides quality of used method of staining 3DHISTECH Ltd assumes no responsibility for the functionality and fault free condition of your application programs Workf...

Страница 5: ...ess loss of business information pecuniary loss or similar damage that stems from or is associated with the incorporated part of this Product that is made by Silicon Graphics Inc THE SOFTWARE IS PROVIDED AS IS AND WITHOUT WARRANTY OF ANY KIND EXPRESSED IMPLIED OR OTHERWISE INCLUDING WITHOUT LIMITATION ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE IN NO EVENT SHALL SAM LEFFLER...

Страница 6: ...02 ed 1 Safety requirements for electrical equipment for measurement control and laboratory use Part 2 101 Particular requirements for in vitro diagnostic IVD medical equipment DIN EN 55011 2009 Class B Limits and methods of measurement of radio disturbance characteristics of industrial scientific and medical ISM radio frequency equipment Conformity with Directive 98 79 EC is documented by the TMA...

Страница 7: ...key combination Example Cross references to other documents or sections within this document Warning Indicates that you need an additional module to use the feature there are prerequisites for the task or that special care should be taken when using certain feature of the device Important An important piece of information or a recommendation The application will work if you choose not to follow th...

Страница 8: ...e tissue cores are removed from the donor and inserted into a recipient paraffin block Clone Block A duplicate triplicate quadruplicate etc of a recipient block with identical layout and core order TMA Tissue microarrays The tissue microarray consists of an array of cylindrical cores of paraffin embedded tissue that are removed from preexisting donor paraffin blocks PCR Polymerase Chain Reaction a...

Страница 9: ...echanical main unit It has an intuitive operation control via the keyboard and the mouse of a PC connected to the main unit The device measures the height of donor blocks the value is displayed next to the donor block thumbnail image based on which the software calculates the depth of punch drill and insertion Thus embedded cores are placed correctly into the recipient block resulting in an even s...

Страница 10: ...hemes Redesigned settings menu Redesigned about window Faster and more effective barcode reading Advanced search option for CaseCenter users TMA Marker colour on the TMA map Advanced search functionality in Custom export application Tutorial videos in about window December 9 2016 Rev 1 3DHISTECH Ltd 10 92 ...

Страница 11: ...the control computer Accessories box Blocks Base molds for paraffin blocks 2 1 TMA Grand Master Main Unit Front View Figure 1 TMA Grand Master front view 1 Protective housing 2 Door opener button 3 Tool change compartment door 4 Protective window 5 Trash container for paraffin flakes and discarded core material 6 Grips for service compartment door 7 Front power supply switch December 9 2016 Rev 1 ...

Страница 12: ...s must be plugged in as shown in the figure above To connect the main unit with the computer any of the three USB ports can be used Warning All cable connections must be checked for firm seating Defective cables must not be connected to any part and must be replaced with cables in proper operating condition Contact 3DHISTECH support at support 3dhistech com in such cases Important The use of an un...

Страница 13: ... box drill bits punchers and pistons paraffin block base molds accessories and tools for the assembly The size and the content of the box can vary according to your purchase order For further information on the basic accessories see section 9 2 Accessories Important Accessories are classified as consumable goods therefore warranty cannot be applied to these items The life span of tools highly depe...

Страница 14: ...icrosoft Excel or OpenOffice on the control computer 2 4 Installing and Configuring TMA Grand Master Hardware Warning Only qualified personnel may install TMA Grand Master and perform the initial start up procedure 3DHISTECH Service technicians install and configure TMA Grand Master and hand it over to you in operating condition 2 5 Transporting the TMA Grand Master Main Unit Warning If you need t...

Страница 15: ...ide plates of the main unit Figure 4 Transporting handle 4 Place the device into the designated cardboard box onto the bottom protective foam 5 Arrange protective padding on the four top corners 6 Pack the accessories into their box 7 Put the main unit and the accessory box to their designated places in the road box 8 Close the box and tape it December 9 2016 Rev 1 3DHISTECH Ltd 15 92 ...

Страница 16: ...or storing digital slides 3 2 TMA Control Software Installation Important 3DHISTECH service technicians perform the installation and configuration of the TMA Control software The following installation procedure is for information purposes only follow the below steps only if you need to re install the software or upgrade to a newer software version Warning The control PC on which the SW applicatio...

Страница 17: ...aunch the TMAControl_2 6_RTM_x64 exe file that you received on a 3DHISTECH Installation Disc or downloaded in a compressed file 2 Follow the instructions in the installation wizard 3 Accept the End User License Agreement and click Next December 9 2016 Rev 1 3DHISTECH Ltd 17 92 ...

Страница 18: ...oftware 4 The installer notifies you if it cannot find the prerequisites Click Install to start the installation of missing prerequisites 5 Click Next to continue with the installation of the TMA Control software application December 9 2016 Rev 1 3DHISTECH Ltd 18 92 ...

Страница 19: ...t the type of license browse for the license file or select HASP dongle 7 Choose the installation folder for the TMA Control software application Click Browse for locating a different folder than the default one then click Next to continue December 9 2016 Rev 1 3DHISTECH Ltd 19 92 ...

Страница 20: ...ose the Start menu folder 9 After completing the installation of TMA Control click Finish to close the wizard Important After the installation RW access right to database and configuration file must be granted for Admin and Standard users December 9 2016 Rev 1 3DHISTECH Ltd 20 92 ...

Страница 21: ...ation on the control computer 2 Switch off the TMA Grand Master 3 Turn the control computer and the monitor off 4 2 Starting the TMA Control Application You can start the program by double clicking onto the program icon of the desktop Warning If you start the software but the hardware is turned off a warning message appears Switch on the main unit then click Retry in the message box Warning If you...

Страница 22: ... TMA Control software If you start the application for the first time a New Project window is displayed in which you must enter a name for the project If you already have worked on projects previously and you want to create a new project click New and if you want to load a project that has been saved previously click Load in the Project panel that is located at the top right corner of the applicat...

Страница 23: ...ing Date Range and or Diameter filters When writing the name of the project to be loaded suggested projects appear on the list according to the entered text string According to the database driven project handling each time you restart the software even without saving the current process of the actual project before exiting all the data is loaded automatically to restore the previous state Decembe...

Страница 24: ...rocessing method of blocks the normal view includes thumbnails of all recipient tray positions and as for donor blocks the selected tray is displayed in its full capacity The other view mode helps users to identify blocks and their statuses more easily Click at the top of recipient blocks panel and at the right end of donor blocks panel to switch to this view December 9 2016 Rev 1 3DHISTECH Ltd 24...

Страница 25: ...lock was loaded into a slot on a donor block tray its image will appear on the left section of the donor blocks panel at the bottom of the main application window At the donor panel a separator can be dragged sideways and is a used for dividing donor block panel to display TMA block thumbnails that are being processed and that are finished 4 3 1 Main Toolbar The main toolbar of the TMA Control sof...

Страница 26: ...hs Images Layout Barcode and Theme See section 5 11 Settings for more information About window contains details of TMA Control software and includes the following panels Info displays software version information and licensed features User Guide Manuals of TMA Grand Master TMA Master and TMA Master II can be accessed and opened directly by clicking the links December 9 2016 Rev 1 3DHISTECH Ltd 26 ...

Страница 27: ...icking the links NOTE Before opening the videos make sure to copy video files to C Program Files 3DHISTECH TMA Control Videos folder from the installation disk This folder may be hidden and thus requires authentication Links Company and product web page links are displayed here Exit application December 9 2016 Rev 1 3DHISTECH Ltd 27 92 ...

Страница 28: ...may end up breaking the puncher needle Important To ensure the good quality of recipient blocks make sure that the top surfaces of both recipient and donor blocks are parallel to the plastic bottom of the cassettes and there are no dents or cavities in the paraffin layer If any defect is visible cut the top layer of the block to make it even otherwise embedded cores may be damaged after creation f...

Страница 29: ... cassette clean the slot area and the cassette then insert the cassette again For cleaning blocks use regular 37mm size blocks only To avoid contamination cleaning block are used before each PCR sampling A cleaning block is a virgin recipient block from which paraffin core is extracted and placed back with the puncher hence cleaning its surface Upon selecting the cleaning block on the user interfa...

Страница 30: ...dingly The number of cleaning spots depending on the puncher diameter are the following Puncher diameter Number of cleaning spots 0 6mm 252 1mm 144 1 5mm 72 2mm 50 4 4 3 Inserting a block into the main unit 1 Recipient block positions are displayed in a double column on the left side of the screen Tray positions of donor blocks are identified by six buttons A F tray capacity is 10 blocks each Dece...

Страница 31: ...oolbar The light pulsates for about 10 seconds and it is possible to open the door within this time frame countdown is displayed in place of the button 5 Push the door opener button to open the door 6 Press down the flap in front of the block slot 7 Slide the block into position with the slanted side towards the back of the machine 8 Release the flap when the block is in position 9 Close the door ...

Страница 32: ...n area 5 Push the door opener button to open the door 6 Press down the flap in front of the block slot 7 Remove the block from its position and release the flap 8 Close the door 4 4 5 Removing a PCR cassette from the main unit Warning After removing PCR cassettes make sure that cores are not clung to the port edges of the cover plates Before carefully removing the cover plate of a PCR cassette gen...

Страница 33: ...ol exchanger compartment door Figure 7 Tool compartment 4 Loosen the drill chuck locking lever marked with red that fixes the drill chuck in position and remove the drill chuck by lifting it upwards Figure 8 Removing the drill chuck December 9 2016 Rev 1 3DHISTECH Ltd 33 92 ...

Страница 34: ...ards as much as you can 7 Hold the front cog wheel firmly and tighten the rear cog wheel clockwise Make sure with the help of the tool that the upper cog wheel is secured firmly 8 Insert the drill chuck into its position and fix it with the locking lever Ensure that the two cog wheels motor and drill chuck are aligned exactly 9 Loosen the puncher chuck locking lever that keeps the puncher chuck in...

Страница 35: ...r into the puncher chuck matching the size of the piston Tighten the rear cog wheel holding the front cog wheel firmly 12 Insert the puncher chuck into its position and fix it with the puncher chuck locking lever Ensure that the two cog wheels motor and puncher chuck are aligned exactly and move the piston into position making attention to place the piston collar to the groove on the console 13 Cl...

Страница 36: ...ocks you can place additional blocks and mark drilling positions on them so you can work simultaneously with the TMA Grand Master You can create up to twelve identical TMA blocks by taking out one tissue core sample and inserting it into each recipient block Then you can either create several slides from the TMA blocks or you can just keep one of the TMA blocks as a backup When you are filling the...

Страница 37: ... the cassettes with the paraffin blocks are properly inserted into the slots Make sure that the drill bit is of appropriate size and that it is fixed in its position For more information see section 11 Cassette and Block Dimensions 5 2 Working with Recipient Block Layouts 5 2 1 Creating a recipient block layout To create a layout click the arrow or at the upper section of the Layout button next to...

Страница 38: ...alue in the Grid properties section NOTE After insertion the puncher moves sideways to break the core As this movement is not possible in some cases without contacting with an already inserted core please raise the Gap value to prevent contamination and the core from being damaged If setting a lower value than advised the software informs you to raise the value but it does not prohibit the usage o...

Страница 39: ...rea within the array with the mouse by drawing a border around the group of insertion positions Sector area can be modified by dragging the marking border to the desired direction and the entire selection area can be moved to a desired direction by dragging The selected sector can be deleted by clicking the button at the top of the border December 9 2016 Rev 1 3DHISTECH Ltd 39 92 ...

Страница 40: ... time then the device processes the upcoming sector you have created It is available for you to create a TMA block on which the sectors are separated by leaving out rows and columns between the sectors by selection The dividing areas can be selected by drawing with the right mouse button thus turning those spots inactive December 9 2016 Rev 1 3DHISTECH Ltd 40 92 ...

Страница 41: ...ion and set the correct measurements Pattern alignment can be set at the Alignment pane of the window Select the desired method of filling for the specific layout under Filling Direction The icon represents random filling as a filling method for blind studies NOTE When using the Sequential Scoring function of the TMA Module the horizontal default filling method can be applied only others will not ...

Страница 42: ...direction is presented at the top left corner of the recipient block panel For more information on layout labeling see section 5 11 Settings Layout 5 2 2 Modifying a recipient block layout 1 Open Layout Editor window by clicking the button next to the recipient block image 2 To modify a block layout select the name of the desired layout from the drop down list then click Edit 3 Follow steps 3 8 in...

Страница 43: ...rting a block into the main unit 3 In the classic view of the recipient block panel block thumbnail images are refreshed each time after you have inserted a block into a slot First click the block thumbnail then type in a block ID or add a layout In the new view for recipient blocks the following options are available for you to choose from after the block thumbnail image is displayed in the Unuse...

Страница 44: ...ved from its slot and after recording its image the representative image of the slot on the interface is displayed as Empty Warning Make sure that you have selected a correct layout for the inserted block so that corresponding dimension values both of the layout and the block are identical For example if Custom block size option is selected do not choose a layout dimension 24x37 for a block with a...

Страница 45: ... recognized properly the following message appears Click Yes and the Barcode window is opened in which you can modify settings 6 If the recipient block does not have any barcode type in the recipient block ID below the preview image of the recipient block 7 Repeat steps 2 and 5 until you have typed in all the block IDs you want to use 8 Click the schematic picture of the first recipient block you ...

Страница 46: ...layed by default If the label is upside down click 3 The Donor block ID must be entered into the text field below the donor block image After entering the donor block ID and hitting enter or clicking the button the label area disappears automatically Click to make the label area reappear NOTE If you have already imported donor block data from a file the last imported data is used and also merge is...

Страница 47: ...stored on the server Furthermore if other locations have been defined in the Settings window for overlay images more images can be found with an identical ID See section 5 11 Section for more information 4 If a case slide or digital image jpg is found with matching IDs their thumbnails are displayed in a window from which you can select one to use it as an overlay December 9 2016 Rev 1 3DHISTECH L...

Страница 48: ...ck at the bottom right corner of the donor block thumbnail displayed at the donor panel 5 5 Launching the transfer process The TMA Grand Master is capable of creating TMA blocks and core sampling into PCR tubes at the same project or you may as well use any of the two options exclusively If you want to use the PCR sampling function of TMA Grand Master simply insert the necessary amount of PCR cass...

Страница 49: ...s a cleaning block click the icon at the right side of the block thumbnail image When recipient or cleaning blocks donor blocks and PCR cassettes are inserted the transfer process can be started NOTE If using TMA Grand Master in PCR mode only and if necessary it is available for you to insert cleaning blocks into empty slots during the process without interrupting it Sometimes it is required to al...

Страница 50: ...k Close to save settings and exit Image Editor or click Default to restore default values Before starting the transfer process follow the sequence of steps below to place markers on donor blocks from which locations cores will be extracted to the selected recipient positions or PCR tubes 1 To place a PCR marker on a donor block first click the PCR button on the left side of the donor image The num...

Страница 51: ...ove an existing marker click it with the right mouse button The corresponding layout position of the deleted marker will therefore be free for the next defined marker 3 Core transfer from the donor block s to the recipient block or PCR tubes starts after clicking the Start button below the image of the donor block If you have not entered Recipient block ID nor Donor block ID for the selected trans...

Страница 52: ...cess the colors of the cores on the layout varies according to their statuses Color markings of cores and their statuses are as follows transferred processing in queue first available position Additional markers placed on the donor block preview image after the process is started will be displayed as on the recipient preview image Upon clicking Start they will turn to and processed accordingly You...

Страница 53: ...rom the tray as described in section 4 4 4 Removing a block from the main unit and insert new donor blocks in the tray Important Because the compartment door can be opened during the process make sure not to remove a recipient block to which a core will be transferred from a donor block since only donor block operations are monitored by the application If you remove a donor block that is finalized...

Страница 54: ...a block the camera records the block position so the software displays an updated image almost instantly Important Make sure when removing finished donor block if every core selected for PCR transfer has been extracted and loaded into the PCR tube together with the relating PCR tube The following message is displayed to inform you about position number of the donor and the relating PCR tubes Impor...

Страница 55: ...e Slide overlay function it is available for you to select a slide image from various sources and of different formats to overlay the donor image with it The digitized slide image located either manually or automatically can be refined and aligned to match the position of the block image Slide overlay settings can be modified on the Overlay panel of the Settings window For more information on sett...

Страница 56: ...g notes to TMA Markers is not supported To initiate the overlay procedure follow these steps 1 Enter donor block ID and hit Enter key or click 2 Click NOTE When hitting Enter the search for the appropriate slide image will not be started 3 If connected to a CaseCenter server the images of the related case will be displayed If more than one images are found in the set locations slide JPG CC then se...

Страница 57: ...ption At other images types make sure that the image of the entire block is captured and saved NOTE CaseCenter server connection can be established only if the URL of the dedicated CaseCenter server is displayed CaseCenter address cannot be modified if displayed For setting server connection details click Login at the main menu bar of the TMA Control window To connect to a dedicated CaseCenter ser...

Страница 58: ...e applied on the donor image NOTE If you modify the donor ID and you have not yet placed any marker on the image the applied overlay slide image will be deleted 7 Slide image is rotated to the appropriate orientation automatically but if you would like to select the matching slide image with a different automatic orientation click at the top of the overlay editor button 8 Furthermore if automatic ...

Страница 59: ...al Position slide image drag rotate zoom to correspond with the block image If manual positioning does not result in an acceptable overlay enable the function Add Reference Points by clicking the button so that it turns green Place two reference points on the block image and refine their positions on the slide image When moving the reference points the donor slide image is automatically aligned If...

Страница 60: ...or block image in the main window Confirm marker positions by clicking on them Close spots will be automatically dragged to the marker positions by the software NOTE By holding the Ctrl key while placing the marker it will not be dragged to close spots automatically By setting the sufficient amount of transparency of the image layer marker positioning becomes easier To modify the opacity level of ...

Страница 61: ... for easier identification of tissue types based on the color chart of TMA Markers in CaseViewer see below image Color RGB values Red 255 0 0 Yellow 255 255 0 Green 0 128 0 Blue 0 0 255 For other colors and their RGB values please follow this link http www rapidtables com web color RGB_Color htm 7 If you have finished press the Start button to launch the transfer process 8 You can refresh the imag...

Страница 62: ...ck Import at the main menu bar of the application window 2 Click Browse in the Import window to search for the data file to be imported If the ID of the donor block loaded into the device exists in the imported database the data set of the specific donor block can be appended to the database December 9 2016 Rev 1 3DHISTECH Ltd 62 92 ...

Страница 63: ...calInfos data sheet can be modified with the Merge or the Replace function The imported data are stored in the MedicalInfos data sheet that can be selected as a record when exporting 4 Select Replace to overwrite existing data with the new ones December 9 2016 Rev 1 3DHISTECH Ltd 63 92 ...

Страница 64: ...other data will be overwritten 6 Differences in the data for example if donor data of a specific block do not match name age etc are highlighted with red in the window The database cannot be modified manually but can be saved with the Save for Editing option Click Save to Database to save imported data 5 8 Restore medical information database The program makes it available for you to restore a med...

Страница 65: ... or TMA only or PCR mode only the Export Window contains different fields and options for export settings 1 In mixed or TMA mode after the TMA blocks are created and you want to save block related data click the Export button in the main toolbar and the Custom Export window appears Select a project from the Project drop down list You can filter the list by activating and specifying the tool diamet...

Страница 66: ... if there is no data imported yet NOTE previously imported clinical data can be exported together with TMA datasets Save scheme by entering a name to the Scheme name field and clicking Save and Use 2 Moving blocks from the Recipient Blocks field to the Coherent Blocks field one by one by clicking creates separate databases for each block Click the button to move all recipient blocks to the Coheren...

Страница 67: ...olumns Unique TMA Sample ID which is the position of the tissue core in the recipient block the TMA Block ID if you are using several blocks in the same project and the Donor Block ID As the TMA Module cannot handle special characters of TMA Block ID fields of TMA Block ID column contains automatically generated names that can be recognized and used by the TMA Module TMA Block Name as an extra col...

Страница 68: ...r positions in the layout The third sheet contains recipient block related data table where the Donor Block IDs are shown according to their positions in the layout Tip If you want to import the exported data to TMA Module you need to create a worksheet called Slides including stain types recipient block IDs and slide names December 9 2016 Rev 1 3DHISTECH Ltd 68 92 ...

Страница 69: ...ath Type and browse for the location of the database file you are about to create 1 Type a database name at the relevant field if you want create a new file 2 Click Browse to set the destination folder of the file and select the file type NOTE When opening XLS files in OpenOffice links to external images cannot be opened 3 Click Save to create the output file or Cancel to exit the window without s...

Страница 70: ...oject name Recipient Block Name Donor Block Name Diameter and Medical Information data In the Date section the date of the first created project is applied as starting date In the Search section Project name Recipient name Donor name and Diameter preferences can be defined During entering a text string in any of the fields corresponding values are listed below the field December 9 2016 Rev 1 3DHIS...

Страница 71: ...ilable to display Recipient and PCR core data in the table In the Medical information section the number of search criterion is defined by the number of medical information field Results related to the set filter parameters automatically appear on the list at the right side of the window Table columns can be reordered by dragging and their order is invertible by clicking the header Search Result t...

Страница 72: ...hich it is available for you to remove all the blocks donor and recipient from the device in one session After clicking Yes first open the door remove blocks then close the door Once the door is closed and the drum turns to the next tray position repeat procedure until all the blocks have been taken out from the device If all the blocks have been removed the software closes the project If you want...

Страница 73: ...o search for slide and JPG images directly in local folders you have set under the sections Set local Slide folders or Set local JPG folders Activate Auto Search In CaseCenter to add results for Overlay function based on Folder Case Block and or Slide criteria Type in or browse for folder paths for automatic search either for Slides or JPG images Folders that have been set can be displayed after c...

Страница 74: ...pth of insertion into the recipient block can be set either by setting a negative or a positive value so that the tip of the puncher is positioned below or above the surface of the block inserting the core at a required height Value ranges from 500μm to 500μm Punching depth μm Punching depth adjustment might be necessary to perform an accurate core removal depending on the location of the tissue w...

Страница 75: ...ind that the puncher needle should be moved with care deeper than the level of the base plate of the cassette since the needle can be damaged during core removal Images Upon activating the Show image related functions option a button will be displayed below the bottom right corner of the block images By clicking this button the Image Editor window can be opened With the Save current Block Image op...

Страница 76: ...esented in this panel Labeling display Activate the Show row and column labeling on layout option to display labels in layout editor and on the recipient block image Labeling variations Click to switch between the following predefined labeling variations for rows columns 0 1 2 3 1 2 3 a b c December 9 2016 Rev 1 3DHISTECH Ltd 76 92 ...

Страница 77: ...et the identifiable type of barcode that is specified manually select Manual or activate Autodetect to allow the program the automatic identification without specifying the barcode type thus blocks with any kind of barcode will be identified during creating a project For software versions older than 2 6 the default barcode reader is selectable after the upgrade Select VintaSoft to process barcodes...

Страница 78: ...D 1D 1D CDV and the subtype of the specific group from the drop down list Theme The graphic user interface now has different types of visualization skins select a preferred skin type by clicking the arrows left of right The elements according to the selected skin type are displayed in the Visual experience section of the window December 9 2016 Rev 1 3DHISTECH Ltd 78 92 ...

Страница 79: ...tly moistened piece of cloth You may also use detergent Wipe off oil or grease contamination traces from the surface of the main unit with a cotton swab dipped into acetone but keep away acetone ethanol from mirrors and lenses It is forbidden to clean mirrors and lenses with ethanol acetone The user should prevent any kind of fungal contamination on the opto mechanical parts of the TMA Grand Maste...

Страница 80: ...compartment do the following 1 Switch off the TMA Grand Master device 2 Pull out the trash container 1 Figure 13 Trash container 3 Release lock by pulling out the lever 1 at the inner panel and move it to the marked position 2 Figure 14 Opening the service compartment door 4 Grab the handles and open the compartment door 5 Clean the compartment area 6 Close the door then lock the lever December 9 ...

Страница 81: ...cable 2 The fuse compartment is located next to the main power supply switch at the back side of the TMA Grand Master 3 Release lock 1 by pushing the lever to the right so the insert 2 can be pulled out Figure 15 Releasing the lock of the fuse compartment 4 Remove insert from the compartment Figure 16 Removing the fuse compartment December 9 2016 Rev 1 3DHISTECH Ltd 81 92 ...

Страница 82: ... 250V fuses only 6 4 Contaminated Tray or Touch Sensor Surface If during initialization after switching on the TMA Grand Master device the program detects possible contamination at the surface of the F donor block tray between the positions F9 and F10 or of the touch sensor the following message appears on the screen Click Yes then follow the instructions regarding cleaning the tray surface Decemb...

Страница 83: ...nsor must be cleansed as well According to the instructions in the following message by using a dry and clean cloth gently rub the bottom surface of the touch sensor sensor is marked with red in Figure 18 Figure 18 Touch sensor After you finished with the cleaning tasks you receive the following message December 9 2016 Rev 1 3DHISTECH Ltd 83 92 ...

Страница 84: ...Contaminated Tray or Touch Sensor Surface Block Image in the Application is of Poor Quality Cause Imaging errors What to do Contact 3DHISTECH support Puncher Falls Out During Punching Cause The puncher needle is detached from the collar What to do Use another puncher The Puncher Does Not Pick Up the Core Cause a The extracted tissue sample is placed too deep into the paraffin it touches the head o...

Страница 85: ...dows 7 after the update process is finished Follow the below steps to avoid the automatic reboot of your PC after a major update via Windows Update Go to Start menu Control panel Windows Update Change settings Select Download updates but let me choose whether to install them item from the Important updates drop down list Click OK to accept changes and close the window December 9 2016 Rev 1 3DHISTE...

Страница 86: ...ecommended to use Defer feature upgrade option that can delay feature updates and security updates will be available only HDD is inaccessible not writable The System Administrator must ensure accessibility by the control software to some folders on the computer For instance write access must be granted to C ProgramData 3DHISTECH TMAControl folder its sub folders files and in addition enable the so...

Страница 87: ...MA software application If the problem still persists try to reinstall it Camera Error message is received Cause Camera cables are not connected properly Solution Follow instructions in the message box Important The folder in which log files are contained is C ProgramData 3DHISTECH TMAControl_LOG Log files can be updated by running the log_refresh bat file December 9 2016 Rev 1 3DHISTECH Ltd 87 92...

Страница 88: ...h of the block is 13 5 mm The smaller size 7 5 mm in depth means the paraffin thickness from the upper edge of the plastic cassette The height of paraffin blocks in parentheses means their total depth together with the plastic cassette Warning Do not use paraffin blocks which dimension values are higher than 24mm W x 37mm H x 10mm D otherwise the drilling and punching heads may be damaged If the p...

Страница 89: ...12 44 352 Max 3 3 approximate values 9 2 Accessories Accessory Dimensions in mm Number of items Base mold for paraffin blocks 37 24 7 5 3 Drill bit Ø 0 6 Ø 1 0 Ø 1 5 Ø 2 0 2 2 2 2 Puncher Ø 0 6 Ø 1 0 Ø 1 5 Ø 2 0 3 3 3 3 Puncher chuck and Drill bit chuck N A 2 Piston Ø 0 6 Ø 1 0 Ø 1 5 Ø 2 0 1 1 1 1 For Drill bits Punchers and Pistons there are four distinctive color markings for each different size...

Страница 90: ...lion Pixel 9 4 Ambient Conditions Condition Suitable Range Shipment packed Ambient temperature 40 C to 70 C Storage Ambient temperature 10 C to 40 C Maximum relative air humidity 75 at 35 C Operation Ambient temperature 10 C to 40 C Maximum relative air humidity 75 at 35 C Maximum altitude 2000 m Air pressure range 500 hPa to 1060 hPa Degree of pollution 2 December 9 2016 Rev 1 3DHISTECH Ltd 90 92...

Страница 91: ... IP 20 Electrical safety in compliance with EN 61010 1 IEC 61010 1 and taking CSA UL regulations into account Over voltage category II Radio interference suppression in accordance with EN 55011 class B Noise immunity in accordance with EN 61326 2 6 2005 Main unit line input voltage 24 V DC 1 V Mains supply voltage 90 240V Mains frequency 50 60 Hz Power rating 300W December 9 2016 Rev 1 3DHISTECH L...

Страница 92: ...39 41 42 43 44 49 50 51 70 M MRXS 8 P PCR 8 9 29 30 36 45 47 48 49 50 53 Punch 9 13 28 29 30 31 32 34 35 36 37 38 45 51 52 83 86 87 R Recipient block 8 9 29 30 31 32 36 37 42 43 44 45 48 49 50 52 64 68 70 78 Research project 9 S Slide 4 8 31 36 50 54 56 57 58 59 70 T TMA 8 9 14 59 68 70 TMA Control 16 18 19 20 21 29 32 43 54 TMA Grand Master 6 9 11 12 13 14 21 32 36 37 38 43 47 48 50 54 78 79 80 8...

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