Zeiss LSM800-2 Upright confocal microscope August 6, 2019
I.
System description:
Upright confocal microscope ZEN blue 2.6/ Hotfix-3
Motorized stage for multiple position imaging
Heating chamber
NO CO
2
mixer
Multislide holder, dish holder, single slide holder
Objectives:
-
Position1:
Objective Plan-Apochromat
20x / NA 1.0 W
DIC (WD=1.8mm)
-
Position2: Objective Plan-Apochromat
10x / NA 0.45 Air
(WD=2.1mm)
-
Position3: Objective
Plan-Apochromat
40x/1.4 Oil DIC
(WD=0.13 mm)
-
Position4: Objective Plan-Apochromat
20x / NA 0.8 Air
-
Objective Plan-Apochromat 63x / NA 1.4 Oil (not mounted)
-
Objective Plan-Apochromat 40x / NA 1.2 Water (not mounted)
-
Laser lines: 405, 488, 561, 640 nm
Two very sensitive Gallium arsenide phosphide photomultiplier tube (GaAsP PMTs) detectors with free choice of the
spectral range. (Uses a tunable short pass and bandpass filter in front of the PMTs)
Be very careful with using it to prevent instrument damage! If oversaturated, detectors will switch off automatically.
An ESID detector for transmitted light.
Simultaneous detection of only two dyes possible, more than two have to be acquired sequentially
II.
Starting up:
1.
Switch on the multiple socket (1). Provides power to the computer and microscope controllers
2.
Switch on SYSTEM (2).
3.
Switch on COMPONENTS (3).
4.
Make sure that lasers are on. Laser key should be in position “I”. (4)
5.
Switch on the computer.
6.
Turn on Cool LED (by pressing
Big blue on/off button
), if you need it for visual examination and don’t
forget to close the shutter when you don’t need it or at the end of your session.
