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Quick Start Manual

Zeiss LSM800-2, Quick Start Manual

The Zeiss LSM800-2 is a cutting-edge microscope, delivering high-resolution imaging for various scientific applications. To get started quickly, the Quick Start Manual provides detailed instructions. You can easily download this comprehensive manual for free from our website, ensuring a smooth and hassle-free user experience.

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5.

Switch off COMPONENTS (3).

6.

Switch off SYSTEM (2).

7.

Switch off multiple sockets (1).

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Summary of Contents for LSM800-2

Page 1: ...nhole and pixel sizes as well as the sectioning thicknesses are all incorrect IMPORTANT LSM800 upright has two new GaAsP PMT detectors As already explained during intros the new PMT are very sensitive and easy to oversaturate and damage GaAsP detectors are shutting down if oversaturated terminating your experiment without further warnings Please use them correctly ...

Page 2: ...ive Gallium arsenide phosphide photomultiplier tube GaAsP PMTs detectors with free choice of the spectral range Uses a tunable short pass and bandpass filter in front of the PMTs Be very careful with using it to prevent instrument damage If oversaturated detectors will switch off automatically An ESID detector for transmitted light Simultaneous detection of only two dyes possible more than two hav...

Page 3: ...equential best signal scan press Apply after choosing the appropriate setup 4 If you are using the detector for transmitted light activate the ESID Detector represented in the light path scheme Make sure Köhler illumination is set properly if you are getting bad bright field images 5 Go to Live acquisition modus and find the focus manually 6 Set pinhole to Airy 1 in the channel dialog to achieve c...

Page 4: ...es You can choose different regions to scan Choose regions and press button to add it Press start experiment Advanced Set up allows you to do preview scan of region of interest 14 Experiment designer Enable Experiment designer Experiment designer dialog will open Design your experiment using all options z stack tiles etc Choose how many blocks you want to have You can add loops Press Start experim...

Page 5: ...5 Switch off COMPONENTS 3 6 Switch off SYSTEM 2 7 Switch off multiple sockets 1 ...

Page 6: ...ser should be avoided at all times Laser safety goggles are situated at all workspaces and should be used in any situation where potential contact of eyes with Laser light of the classes 3B or 4 is possible according to the previous two points Laser safety goggles have to be worn at all times of operation of Laser Class 4 Laser safety goggles have to be worn at all times of operation laser Class 3...

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