Note: Acquiring more pixels than ”optimal” almost never makes sense, but feel free to decrease the
pixel number to increase acquisition speed at the cost of resolution.
10.
To capture single images, click at ‘Snap’.
11.
Setting up Z-stack:
-
Enable Z-Stack and the Z-Stack dialog will appear
-
Press “Live” and find first and last slices with the focus knob and select them in the software accordingly.
-
The software will suggest the optimal slice number and interval which you can change manually
-
Press “Start experiment”
12.
Setting up time series:
-
Enable “time series”
-
Time series dialog will open
-
Set cycle number and interval time
-
Press “start experiment”
13.
Tile Scan (in this mode you have: Tiles, Multipositions)
-
Enable “tile scan”
-
Tile scan dialog will open
-
Set desired number of horizontal and vertical tiles
-
You can choose different regions to scan. Choose regions and press button “+” to add it.
-
Press “start experiment”
Advanced Set up allows you to do preview scan of region of interest.
14.
Experiment designer.
-
Enable “Experiment designer”.
-
Experiment designer dialog will open
-
Design your experiment using all options (z-stack, tiles etc). Choose how many blocks you want to have. You
can add loops.
-
Press “Start experiment”
15.
Data Saving:
Save your data to D: DATA folder in your own folder. Note that this computer is not backed Up. Please move
your data as soon as possible using portable storage devices or connect with intranet.
You can also use “Auto save”, your experiment and snaps will go directly to your folder.
V.
Switching off:
1.
DO NOT save any “Experiment” or overwrite the defult “Experiment” (marked with a home logo) as this setting
will be used in the next start up of the system and might cause potential damage to GaAsP PMTs.
2.
Close the ZEN Software
3.
Shut down the computer.
4.
Turn off the cool LED (5)
