Zeiss Axiovert 40, Operating Manual

Page: 42 / 72

Axiovert 40
11/03 B 46-0031 e
2Ć16
• The mid-position of the slider Ph, H, Var and the slider Var, H, Var allows brightfield illumiĆ
nation.
• The left click-stop position of the slider Ph, H, Var allows fast change from VAREL to phase
contrast.
Fig. 2Ć20 Pupil images with VAREL
Objective VAREL Oblique brightfield
illumination
Unilateral darkfield
illumination
phase ring VAREL ring
VAREL diaphragm
Preparing the slider Var, H, Var for phase contrast
• Remove the attenuation filter (2Ć21ā/ ā3 ) from the
central mount of the slider (remove spring ring
and filter).
• Insert the ring diaphragm mount (2Ć21ā/ā 1 ) (supĆ
plied with the slider). If necessary, loosen centerĆ
ing screws (2Ć21ā/ā 2 ) by means of the key.
• Insert ring diaphragm in ring diaphragm mount
and center it to the phase ring of the objective usĆ
ing the centering screws (one centering screw at
each side of the slider).
Objective VAREL diaphragms for
condenser 0.55 condenser 0.4 condenser 0.2
A-Plan 10x/0.25 Ph 1 Var 1 Var 1/0.55 451252 Var 1/0.4 451251
LD A-Plan 20x/0.30 Ph 1 Var 1 Var 1/0.55 451252 Var 1/0.4 451251
LD A-Plan 32x/0.35 Ph 1 Var 1 Var 1/0.55 451252 Var 1/0.4 451251
LD A-Plan 40x/0.50 Ph 2 Var 2 Var 2/0.55 451253
Fig. 2Ć21 Slider Var, H, Var
1
2
3