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Axiovert 40

11/03 B 46-0031 e

3Ć4

(3) Replacing the HBO 50

The HBO 50 microscope illuminator is used for incident-light fluorescence excitation. It comprises the followĆ

ing items:

•

HBO 50 illuminator

447220-0000-000

•

HBO 50/SF 20 collector

447271-0000-000

•

HBO 50 mercury vapor short-arc lamp

381619Ć0000-000

•

Transformer mbq52ac-z for HBO 50ac

000000-1113-833

Fig. 3Ć3

HBO 50 illuminator with line spectrum

Wavelength in nm

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Summary of Contents for Axiovert 40

Page 1: ...Operating Manual Axiovert 40 Inverted Microscope...

Page 2: ......

Page 3: ......

Page 4: ...tents are not permitted Violations will entail an obligation to pay compensation The manual is not covered by an update service Number of this manual B 46 0031 e Date of issue 11 25 2003 Issued by Car...

Page 5: ...Contents I List of illustrations IV Note VI General view VII Notes on instrument safety IX 1 DESCRIPTION 1 1 Name and intended use 1 1 1 2 Instrument description 1 1 1 2 1 Mechanical design 1 3 1 2 2...

Page 6: ...1 2 4 2 Brightfield illumination 2 12 2 4 3 Phase contrast 2 13 2 4 4 VAREL 2 15 2 4 5 PlasDIC 2 17 2 4 6 Incident light fluorescence 2 19 2 5 Working with attachable object traverser M and mounting f...

Page 7: ...Axiovert 40 B 46 0031 e 11 03 III Page 3 MAINTENANCE AND TROUBLESHOOTING 3 1 Maintenance 3 1 3 2 Troubleshooting and service 3 2 ANNEX Index A 3 List of abbreviations A 7...

Page 8: ...ower outlet 2 5 Fig 2 8 Inserting the eyepieces 2 6 Fig 2 9 Positions of the binocular tube 2 7 Fig 2 10 Inserting an eyepiece reticle 2 7 Fig 2 11 Switching on setting the instrument 2 8 Fig 2 12 Wor...

Page 9: ...ital camera adapter 2 26 Fig 3 1 Checking the line voltage 3 2 Fig 3 2 Replacing the halogen bulb 3 3 Fig 3 3 HBO 50 illuminator with line spectrum 3 4 Fig 3 4 Replacing the bulb of the HBO 50 3 5 Fig...

Page 10: ...rked by a reference line and an item number For example eyepiece tube 2 8 4 means that the eyepiece tube in figure 8 in section 2 is marked with the item number 4 Abbreviations are explained in the An...

Page 11: ...Axiovert 40 B 46 0031 e 11 03 VII GENERAL VIEW Axiovert 40 CFL...

Page 12: ...Axiovert 40 11 03 B 46 0031 e VIII...

Page 13: ...Conformity to the given standards is documented by the marking This operating manual includes information and warnings that must be observed by the user NOTE This symbol is a warning which you must ob...

Page 14: ...ltages to be used in the range between 100 and 240 V AC 10 50 60 Hz without the voltage setting on the instrument having to be changed Before switching on the HBO 50 power supply check whether it is s...

Page 15: ...hrough the ventilation slats The instruments may only be operated by trained personnel who must be aware of the possible dangers involved with microscopy and the particular application concerned The A...

Page 16: ...Axiovert 40 11 03 B 46 0031 e XII...

Page 17: ...and or tissue specimens but also of specimens of the human body Observation and cultivation vessels include for instance culture bottles Petri dishes and microtiter plates The following microscopy tec...

Page 18: ...field to oblique brightfield contrast ing of the wells of microtiter plates up to the edge without special preparations PlasDIC for relief like imaging especially of thick objects with variable contra...

Page 19: ...djustable to two heights with one fixed and one focusing eyepiece Quintuple nosepiece Specimen stage prepared for fitting the attach able object traverser M with the possibility of us ing different mo...

Page 20: ...ustomization to the intended application Depending on the configuration the Axiovert 40 microscopes are equipped with a 12 V 35 W halogen lamp and a HBO 50 illuminator An universal port is available f...

Page 21: ...ns Transport in packaging Permissible ambient temperature 40 to 70 C Storage in packaging Permissible ambient temperature 10 to 40 C Permissible relative humidity v 75 Operation Permissible ambient te...

Page 22: ...max 350 VA Fuses acc to IEC 127 Microscope stand Axiovert 40 C T 1 A H 5x20 mm Mikroskopstativ Axiovert 40 CFL T 1 A H 5x20 mm mbq 52 ac z transformer for HBO 50 100 V to 127 V 2x T 4 A 220 to 240 V...

Page 23: ...5 adjustable aperture diaphragm with scale max aperture 0 2 0 4 or 0 55 mount for diaphragm sliders Objectives ICS objectives with W 0 8 x 1 36 thread Objective change manual via quintuple nosepiece E...

Page 24: ...Axiovert 40 11 03 B 46 0031 e 1 8 1 4 System overview Fig 1 3 1 Axiovert 40 system overview...

Page 25: ...Axiovert 40 B 46 0031 e 11 03 1 9 Fig 1 3 2 Axiovert 40 system overview...

Page 26: ...Axiovert 40 11 03 B 46 0031 e 1 10 Fig 1 3 3 Axiovert 40 system overview...

Page 27: ...e cardboard box 2 1 1 containing the accessories Holding the polyethylene packing 2 1 2 of the microscope stand at the grip openings 2 1 3 re move it from the cardboard box and place it on the side Re...

Page 28: ...2 3 1 located above the nosepiece NOTE Filter slider 2 2 3 is firmly mounted the appropriate filters green filter attenuation filter are not inserted during transport 2 Screwing in of objectives Remov...

Page 29: ...denser objective combination used refer to the table on page 2 14 4 Mounting the HBO 50 illuminator Attach the HBO 50 illuminator 2 5 3 equipped with mercury vapor short arc lamp to the corre sponding...

Page 30: ...ct the mounting frame 2 6 1 appropriate for the specimen vessel to be used and fit the corre sponding scales in the recesses on the attachable object traverser M and adhere them NOTE When using the mo...

Page 31: ...3 on the right of the instrument NOTE When the instrument is switched off the 0 marking is visible on the power switch The instrument is ready for operation when the microscope lamp is on In the even...

Page 32: ...g eyepiece 2 8 3 turn its eyelens until the specimen is in focus for the other eye too 2 With two focusing eyepieces Turn the eyelens of the first focusing eyepiece 2 8 3 to focus on the eyepiece reti...

Page 33: ...or use with reticles The slight image shift produced by the additional path through the glass is taken into account on the diopter scale by the fact that the zero position is indicated by the red dot...

Page 34: ...on camera path selector switch 2 11 7 2 2 Start up When starting up the Axiovert 40 microscope for the first time the instrument has to be unpacked in accor dance with Section 2 1 1 installed in accor...

Page 35: ...rking distances FWD on Axiovert 40 Objective Name Catalogue No FWD without coverglass cap for bottom thickness FWD with coverglass cap 000000 1016 757 for bot tom thickness 0 17 0 6 D 0 17 mm D 1 mm D...

Page 36: ...llides with the underside of the specimen stage Objects on a 1 mm thick vessel bottom can be focused if the vessel is moved via attachable ob ject traverser M and mounting frame If these conditions ar...

Page 37: ...40x Microscopic technique Brightfield phase contrast Brightfield phase contrast VAREL 10x up to 32x PlasDIC Brightfield phase contrast VAREL PlasDIC To fully illuminate object fields even when low pow...

Page 38: ...the bottom part of the con denser upwards and clamp it when the maximum illumination intensity in the object field has been achieved 2 4 2 Brightfield illumination Prepare the microscope as described...

Page 39: ...jective ap pears as a gray ring in the bright pupil 2 16 A Fig 2 16 Illustration of phase contrast adjustment A C B Slide the annular diaphragm 2 17 1 of a slider with phase diaphragm positions 2 15 1...

Page 40: ...4 condenser 0 2 A Plan 5x 0 12 Ph 0 Ph 0 0 4 451255 Ph 0 0 2 451261 CP Achromat 10x 0 25 Ph 1 Ph 1 0 55 451258 Ph 1 0 4 451256 Ph 1 0 2 451260 A Plan 10x 0 25 Ph 1 Ph 1 0 55 451258 Ph 1 0 4 451256 Ph...

Page 41: ...or 0 55 con denser up to the VAREL position Swivel in the required VAREL objective Use the adjusting screw 2 18 5 to move the left or right VAREL diaphragm aperture after select ing the engaging posi...

Page 42: ...unt of the slider remove spring ring and filter Insert the ring diaphragm mount 2 21 1 sup plied with the slider If necessary loosen center ing screws 2 21 2 by means of the key Insert ring diaphragm...

Page 43: ...s not necessary to use cultivation vessels with glass bottom Prepare the microscope as described in Sections 2 4 and 2 4 1 Equip the 3 position reflector slider P C with the analyzer module D P C for...

Page 44: ...ntation The relief image provides the best contrast NOTE If no longer used you can plug the PlasDIC slider 2 22 4 for storage into one of the two mounts on the microscope stand below the specimen stag...

Page 45: ...p 2 24 5 at the power supply 2 24 4 but block the light path using the addi tional filter slider 2 24 6 which in its mid position does not allow the light to pass After you have selected the specimen...

Page 46: ...available other mounting frames for COSTAR CORNING and tissue culture flasks Hamax plates Coates plates as well as plankton chambers on request Mounting frame M for 76 x 26 mm specimen slides Mountin...

Page 47: ...tolerance 0 001 mm Catalogue No 4540060 0000 000 Stage micrometer positive 5 100 100y d 0 17 mm Graduation to one side 5 mm in 5 intervals Graduation to other side 1 mm in 100 100 mm 10 mm Accuracy 1...

Page 48: ...underside of the specimen stage Three M5 threaded holes 2 27 2 on the left and right sides of the stand Four M5 threaded holes 2 27 3 on the underside of the stand for securing the stand Refer to the...

Page 49: ...m the stage surface Using the SW 3 ball headed screwdriver remove three fastening screws 2 28 2 and the stage 2 28 3 Fit and fasten the selected specimen stage specimen stage glass Section 2 8 1 heata...

Page 50: ...e microscope stage A heatable stage and a temperable microscope stage are available for the Axiovert 40 microscope Heatable stage 471820 9901 000 including three spacers Temperable microscope stage 41...

Page 51: ...act digital cameras Remove the dust protective cap from the camera body 2 30 1 e g A 70 Canon Fix the camera specific filter adapter 2 30 2 to the camera body The filter adapter is included in the cam...

Page 52: ...in an unmasked image in any zoom position of the camera lens Set the camera as follows Switch off the autofocus Set the distance to infinity Set exposure control to aperture priority auto ex posure Ch...

Page 53: ...g petroleum ether but do not use alcohol Use commercially available optics cleaning cloths to remove stubborn dirt if necessary lightly moisten the cloths with petroleum ether When using the Axiovert...

Page 54: ...aph 1 Replacement of halogen bulb according to paragraph 2 Replacement of HBO 50 according to paragraph 3 Replacement of reflector module according to paragraph 4 1 Checking the line voltage Check pow...

Page 55: ...heat sink 3 2 2 upward Remove the halogen bulb with alignment base 3 2 3 Remove new 12 V 35 W halogen bulb with adjustment base from the packing box Insert it in the mount taking care that the tip of...

Page 56: ...ent light fluorescence excitation It comprises the follow ing items HBO 50 illuminator 447220 0000 000 HBO 50 SF 20 collector 447271 0000 000 HBO 50 mercury vapor short arc lamp 381619 0000 000 Transf...

Page 57: ...ime of the HBO 50 can be read from the hour meter This warning label on the rear of the illuminator means Caution Hot surface Allow the lamp to cool down before touching it Replacing the HBO 50 lamp C...

Page 58: ...g the glass bulb remove fingerprints immediately if necessary Depress spring loaded lever and holding the lamp only at the heat sink insert the assembly of lamp and heat sink into the holder Slowly re...

Page 59: ...ge Turn knurled knob 3 5 4 to focus the collector until the brighter light arc is imaged sharply Use adjusting screw 3 5 1 to adjust the lamp ax ially to the reflector until both light arcs are equall...

Page 60: ...the Price List Remove 3 position reflector slider P C from Ax iovert 40 CFL microscope Loosen fastening screws 3 7 3 on the right and left and remove cover 3 7 1 upward To remove a reflector module F...

Page 61: ...citation filter in 3 8 5 and lock them in place by means of the retaining rings 3 8 1 Barrier filters and excitation filters may be marked on their periphery with their names and an arrow The arrow in...

Page 62: ...dichroic beam splitter follow this proce dure Use the screwdriver to loosen the two slotted screws 3 10 1 Hold both halves of the module emission half 3 10 2 and excitation half 3 10 3 of the reflec t...

Page 63: ...the module holding ele ments 3 11 5b and eyes 3 11 5a interlock Hold ing both halves together turn them over in mounting position again Reinsert the slotted screws and tighten them Finally affix the a...

Page 64: ...40 may only be performed by Carl Zeiss service staff or specially authorized personnel To ensure optimum setting and trouble free function of your microscope over a longer period of time we recommend...

Page 65: ...Axiovert 40 B 46 0031 e 11 03 A 1 ANNEX Index List of abbreviations...

Page 66: ...Axiovert 40 11 03 B 46 0031 e A 2...

Page 67: ...Brightfield illumination 2 12 Bulb replacement Halogen lamp 3 3 C Changing the dichroic beam splitter 3 10 Condenser 2 11 Corr objectives 2 10 Counting reticles 2 21 D Defective vision 2 6 Design Mech...

Page 68: ...O 50 illuminator 2 3 Heatable stage 2 23 2 24 I Illumination and contrast techniques 2 11 Immersion objectives 2 10 Incident light fluorescence 2 19 Installation 2 2 Instrument features 1 2 Instrument...

Page 69: ...2 15 Mounting frames 2 4 2 20 O Object traverser M 2 4 2 20 Operating data 1 5 Operation 2 1 Optical diagram 1 4 Optical mechanical data 1 6 Optics 1 4 P Phase contrast 2 13 Phase contrast adjustment...

Page 70: ...23 2 24 Specimen stage replacement 2 23 Start up 2 8 Switching on the instrument 2 8 System overview 1 8 T Technical data 1 5 Temperable microscope stage 2 23 Transformer for HBO 50 mbq 52 ac z 1 6 T...

Page 71: ...focusing fot Photographic FT Dichroic beam splitter FWD Free working distance HAL Halogen lamp HBO Mercury vapor short arc lamp HF H Brightfield ICS Inifinity Colour corrected System IEC International...

Page 72: ...Axiovert 40 11 03 B 46 0031 e A 8...

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