Axiovert 40
B 46-0031 e 11/03
2Ć19
2.4.6
Incident-light fluorescence
Incident-light fluorescence requires the use of the Axiovert 40 CFL microscope with the following compoĆ
nents:
FL incident-light equipment, HBO 50 illuminator with mbq ac-z transformer, 3-position reflector slider P&C
and reflector module FL P&C (equipped with set of filters for fluorescence excitation).
•
Select the specimen area to be examined in transmitted light brightfield or phase contrast. To do this, push
reflector slider (2Ć24ā/ā
10
) to the blank position, switch on halogen bulb (2Ć24ā/ā
2
) and move diaphragm slider
(2Ć24ā/ā
3
) to brightfield or phase contrast position.
•
Switch on the HBO 50 lamp (2Ć24ā/
ā5
) at the power supply (2Ć24ā/ā
4
), but block the light path using the addiĆ
tional filter slider (2Ć24ā/ā
6
), which in its mid-position does not allow the light to pass.
•
After you have selected the specimen area of interest, switch off the halogen bulb.
•
With the 3-position reflector slider P&C (2Ć24ā/ā
10
) move the required filter combination (2Ć24ā/
ā9
) into the
light path and release the light path by removing the additional filter slider (2Ć24ā/ā
6
).
•
Move the push-pull rod (2Ć24ā/ā
7
) to close the luminous-field diaphragm until it is visible in the field of view.
Center it by means of centering screws (2Ć24ā/ā
8
) and open it up to the edge of the field of view.
•
In the additional filter slider (2Ć24ā/ā
6
) of the FL incident-light equipment, you can insert additional 25-mm
excitation filters, which must be held by means of O-rings.
Fig. 2Ć24
Observation in incident-light fluorescence
Summary of Contents for Axiovert 40
Page 1: ...Operating Manual Axiovert 40 Inverted Microscope...
Page 2: ......
Page 3: ......
Page 11: ...Axiovert 40 B 46 0031 e 11 03 VII GENERAL VIEW Axiovert 40 CFL...
Page 12: ...Axiovert 40 11 03 B 46 0031 e VIII...
Page 16: ...Axiovert 40 11 03 B 46 0031 e XII...
Page 24: ...Axiovert 40 11 03 B 46 0031 e 1 8 1 4 System overview Fig 1 3 1 Axiovert 40 system overview...
Page 25: ...Axiovert 40 B 46 0031 e 11 03 1 9 Fig 1 3 2 Axiovert 40 system overview...
Page 26: ...Axiovert 40 11 03 B 46 0031 e 1 10 Fig 1 3 3 Axiovert 40 system overview...
Page 65: ...Axiovert 40 B 46 0031 e 11 03 A 1 ANNEX Index List of abbreviations...