Zeiss Axiovert 40, Operating Manual

Page: 35 / 72

Axiovert 40
B 46-0031 e 11/03 2Ć9
2.3 Use of LD objectives
The bottom thickness of the vessels typically used for
examination on inverted microscopes considerably
differs from the standard coverglass thickness of
0.17 mm.
Normally, such distances are easily bridged by the
free working distance (FWD) of low-power objecĆ
tives:
- CP Achromat 5x/0.12: FWD (in air) 10.9 mm or
- CP Achromat 10x/0.25: FWD (in air) 5.3 mm.
Already in the medium magnification range, howĆ
ever, these working distances are reduced to values
around or below 1 mm. Such objectives can then no
longer be used for thicker bottoms.
This shortcoming is remedied by special LD (Long Distance) objectives. These objectives feature a relatively
long working distance, but also the standard 45 mm parfocalization length of all other objectives.
For adaptation to the bottom thickness in use, plug the corresponding coverglass cap (2Ć12ā/ā
1 ) onto the objecĆ
tive (see table below for assignments).
Table of free working distances (FWD) on Axiovert 40
Objective Name Catalogue No. FWD
without coverglass cap
for bottom thickness
FWD
with coverglass cap
(000000-1016-757) for botĆ
tom thickness 0.17 - 0.6
D=0,17
[mm]
D=1
[mm]
D=1,2 Ć 1,8
[mm]
CPĆAchromat 5xā/ā0.12 440920-0000-000 10.9 10.4 10.2 ... 9.8
CPĆAchromat 10xā/ā0.25 440930-0000-000 5.3 4.8 4.6 ... 4.2
A-Plan 2.5x/0.06 000000-1113-114 9.4 8.9 8.7 ... 8.3
A-Plan 5x/0.12 441020-0000-000 9.8 9.2 9.1 ... 8.7
A-Plan 10x/0.25 441030-0000-000 4.4 3.9 3.7 ... 3.3
LD A-Plan 20x/0.30 Ph 1 000000-1006-591 4.8 4.3 4.2 ... 3.8
LD A-Plan 32x/0.40 Ph 1 000000-1006-593 3.2 3.1 ... 2.7 3.1 ... 2.8
LD A-Plan 40x/0.50 Ph 2 000000-1006-595 2.0 1.9 ... 1.5 1.9 ... 1.6
Plan Neofluar 2.5x/0.075 000000-1113-113 9.55 9.0 8.9 ... 8.5
Plan Neofluar 5x/0.15 440320-0000-000 13.6 13.1 12.9 ... 12.5
Plan Neofluar 10x/0.30 440330-0000-000 5.58 5.0 4.9 ... 4.5
Corr objectives D=0.17 - 1.5
LD Plan Neofluar 20x/0.40 corr Ph 2 441341-9970-000 8.34 ... 7.46
LD Plan Neofluar 40x/0.60 corr Ph 2 441351-9970-000 3.27 ... 2.57
LD Plan Neofluar 63x/0.75 corr Ph 2 441371-9970-000 2.11 ... 1.24
The working distances of PhVar objectives correspond to those of the Ph objectives.
Fig. 2Ć12 Working with LD objectives
1