Zeiss Axiovert 40, Operating Manual

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Axiovert 40
11/03 B 46-0031 e
2Ć12
Height adjustment of the condenser 0.55
The height of the condenser 0.55 can be adjusted in
order to obtain homogeneous illumination of the obĆ
ject field and optimum intensities in all the focusing
positions of the objective.
When the fixation screw has been loosened, the botĆ
tom part of the condenser can be moved relative to
the upper part and clamped in any position.
In phase contrast and VAREL, the phase rings and
stops remain superimposed after the adjustment.
• If the object plane is 0...2.5 mm above the stage
surface, keep the condenser in the lower position.
• If the object plane is more than 2.5 mm above the
stage surface, move the bottom part of the conĆ
denser upwards and clamp it when the maximum
illumination intensity in the object field has been
achieved.
2.4.2 Brightfield illumination
• Prepare the microscope as described in Sections
2.4. and 2.4.1.
• Set slider Ph, H, Ph (2Ć14ā/ā 4 ) to mid-position.
• Place the specimen on the specimen stage and,
using a low-power objective, e.g. the CP AchroĆ
mat 10x, focus on it by turning the focusing drive.
• Swing lever (2Ć14ā/ā 6 ) to close the aperture diaĆ
phragm until the image contrast is optimal.
NOTE Do not use the aperture diaphragm
for controlling the image brightness
(loss in image quality)!
• If necessary, turn control (2Ć14ā/ā 5 ) to change the
lamp voltage and thus brightness or insert an atĆ
tenuation filter (2Ć14ā/ā 1 ) in the filter slider (2Ć14ā/ā 2 )
and push it into the light path.
Fig. 2Ć13 Condenser 0.55
Á
Fig. 2Ć14 Various modes of brightfield
illumination
1 2
3
4
5
6
7