AxioCam ERc 5s
Microscope camera
Operating instructions
Imprint
Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany
[email protected]
www.zeiss.de/axiocam
Carl Zeiss Microscopy GmbH
Königsallee 9-21
37081 Göttingen, Germany
426540-7144-001
Printed 04/2012
© Carl Zeiss Microscopy GmbH, 2010
Control elements, indicators and con-
nections
Front of camera:
1 LED indicator
2 Manual acquisition
3 AWB: Automatic white/
gray balance
4 Receiver for IR remote
control
5 Card slot for SD/SDHC
memory cards
Back of camera:
1 USB port for data trans-
fer and power supply
2 DVI signal via HDMI
port for monitor, TV
or beamer
3 S-Video connection for
recorder or monitor
Definitions
SD memory card:
Digital storage medium for recording image data and
other information. On SD cards an amount of 4 GB of data can be stored; up to
32 GB on new SDHC cards (HC=high capacity). SD cards should be formatted on
a PC to FAT32 format.
C-mount adapter:
The C-mount is a standardized mounting thread for
camera lenses. In microscopy the C-mount is used to connect digital cameras.
So it is guaranteed that the image produced by the microscope objective is large
enough to illuminate the small camera sensors.
AWB (Automatic White Balance):
The purpose of the white balance is to
calibrate the camera to the color temperature of the incident light and thereby
prevent color cast. However, the automatic white balance that is normally
used does not work, or does not work sufficiently well, in the case of technical
surfaces or macros. This is because these objects often do not have any defined
pure-white or color-neutral surfaces that the automatic white balance can take
as a basis for the calibration. Instead it uses the average values of the individual
red, green and blue (RGB) color channels for reference. It is therefore necessary
to perform the white balance manually using defined color-neutral objects.
Parfocality:
According to Professor A. Köhler, the focus plane set should be
retained when eyepieces or objectives are changed on the microscope. Ideally,
the object should appear just as sharp as it did before the eyepiece or objective
was changed. Today, this also applies to the phototube for the acquisition of
images using a digital camera: the sharpness of the camera’s images should
correspond to that of the image you see when you look through the eyepiece. It
is possible to set the parfocality of the camera even with the help of a reticle in
the intermediate image of the eyepiece or with the help of an adapted monitor.
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