5-21
Press [2] for Buffer to turn on the Buffer Pump. The pump runs until you press [2] a second
time. The display does not change messages during this activity. You should hear the pump and
see fluid moving through the Sample Chamber and exiting through the opening of the steel
cone, then through the Waste Tube to the Waste Bottle.
Pump diagnostic
1-SipperPump 2-Buffer 3-Cal 4-ExtPump
Press [3] for Calibrator to turn on the Calibrator Pump. The pump runs until you press [3] a
second time. The display remains as shown below. You should hear the pump and see fluid
moving into the Cal Well of the Sample Chamber Block, and then flowing out through the
Waste Tube to the Waste Bottle.
Pump diagnostic
1-SipperPump 2-Buffer 3-Cal 4-ExtPump
Press [0] to return to the five diagnostic selections.
NOTE: Unless the 2730 Monitor and Control pump is installed, 4-ExtPump will give no
response, Refer to the YSI 2730 Monitor and Control Accessory User’s Manual for details of
external pump operation.
Select diagnostic
1-Motor 2-Pump 3-Probe 4-I/O 5-Sensor
Press [3] for Probe. You will observe a brief display message, as shown below, which then
changes to show probe current(s). You will observe only Black probe current for a single
channel unit. The probe activity is expressed in nA (nanoamperes) of electrical current.
A nanoampere is 10
-9
amperes, which is a very low level of electrical current.
Reading probe current...
Please wait.
B:LAC 0.50 nA W:DEX 1.76 nA
1-Flush 2-Calibrator 3-Sample
NOTE: If you assign "none" for a probe in the Setup (measurement parameters) menu, or you
have a single channel unit, the display appears as shown below.
B:LAC 0.50 nA W: -Not assigned-
1-Flush 2-Calibrator 3-Sample
Press [2] for Calibrator. The Calibrator Pump turns on as the Sipper moves to the Cal Well and
descends. Calibrator solution is aspirated, then moved to the Sample Chamber and dispensed,
just as you would observe in a normal calibration cycle.
The display probe current should rise above baseline and within 20 to 30 seconds should level
off at some nanoampere value indicative of the particular chemistry and aspiration volume you
have setup.
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