4-20
&
# +#
You must have a dual channel version of the 2700 SELECT in order to perform this
measurement. Refer to the previous sections on L-GLUTAMATE and L-GLUTAMINE for
theoretical and special considerations, then follow the instructions below to set up your
instrument for simultaneous determination.
Since the Glutamine Membrane (2735) utilizes a chain of enzyme reactions and the final
reaction is a “glutamate measurement”, free glutamate in a sample will be measured at the
Glutamine Sensor. By using the information at the Glutamate Sensor, this free glutamate is
automatically subtracted in the instruments software. The algorithm looks basically like this:
(“Total Glutamate” - “Free Glutamate”) = Glutamine
Total Glutamate is the combined glutamine and glutamate probe signal. Free Glutamate is the
glutamate not derived from Glutamine reaction.
System Buffer
YSI 2357
Calibrator Std
YSI 2755 and YSI 2736
Linearity Std
YSI 2756 and YSI 2737
Black Membrane
YSI 2735
White Membrane
YSI 2754
Sample Size
20
µ
L
Cal Station
Two Station, #1 (glutamate), #2 (glutamine) *
Black Probe Parameters
White Probe Parameters
Chemistry
Glutamine
Chemistry
Glutamate
Unit of Conc
mmol/L
Unit of Conc
mmol/L
Cal value
5.00
Cal value
5.00
End Point
30 sec
End Point
30 sec
* Since glutamine calibrator is at the Test Tube Holder, you must sample at the Manual Station
(#3). The Turntable position (#4) and the Monitor Station (#5) may also be used on units
equipped with these accessories.
Special Consideration:
»
The ability to select sample size (5 to 65
µ
L) may help you optimize your system,
especially if very high or very low concentrations of one or the other of these
substrates is a problem. The optimal probe current for a calibrator is about 10 nA. See
Menu Diagnostics, Section 5.4, to learn more about measuring your probe signal
before entering Run mode.
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