Waters 2998, Overview And Maintenance Manual

Page: 90 / 94

December 5, 2017, 715004753 Rev. B
Page 90
C.9.2 Mixed mobile phases
The table below contains approximate wavelength cutoffs for some other solvents, buffers,
detergents, and mobile phases. The solvent concentrations represented are those most commonly
used. If you want to use a different concentration, you can determine approximate absorbance
using Beer’s Law, because absorbance is proportional to concentration.
Carbon disulfide 380 Methyl ethyl ketone 330
Carbon tetrachloride 265 Methyl isobutyl ketone 334
Chloroform 245 Methylene chloride 233
Cyclohexane 200
n
-Pentane 190
Cyclopentane 200
n
-Propanol 210
Diethyl amine 275
n
-Propyl chloride 225
Dioxane 215 Nitromethane 380
Ethanol 210 Petroleum ether 210
Ethyl acetate 256 Pyridine 330
Ethyl ether 220 Tetrahydrofuran 230
Ethyl sulfide 290 Toluene 285
Ethylene dichloride 230 Xylene 290
Table C–3: Wavelength cutoffs for different mobile phases
Mobile Phase
UV Cutoff
(nm)
Mobile Phase
UV Cutoff
(nm)
Acetic acid, 1% 230 Sodium chloride, 1 M 207
Ammonium acetate, 10 mM 205 Sodium citrate, 10 mM 225
Ammonium bicarbonate, 10 mM 190 Sodium dodecyl sulfate 190
BRIJ 35, 0.1% 190 Sodium formate, 10 mM 200
CHAPS, 0.1% 215 Triethyl amine, 1% 235
Diammonium phosphate, 50 mM 205 Trifluoroacetic acid, 0.1% 190
EDTA, disodium, 1 mM 190 TRIS HCl, 20 mM, pH 7.0,
pH 8.0 202, 212
HEPES, 10 mM, pH 7.6 225 Triton-X™ 100, 0.1% 240
Hydrochloric acid, 0.1% 190
Waters PIC
®
Reagent A,
1 vial/liter
200
MES, 10 mM, pH 6.0 215 Waters PIC Reagent B-6,
1 vial/liter 225
Potassium phosphate,
monobasic, 10 mM
dibasic, 10 mM
190
190
Waters PIC Reagent B-6, low UV,
1 vial/liter 190
Sodium acetate, 10 mM 205 Waters PIC Reagent D-4,
1 vial/liter 190
Table C–2: UV cutoff wavelengths for common chromatographic solvents (continued)
Solvent UV Cutoff (nm) Solvent UV Cutoff (nm)