December 5, 2017, 715004753 Rev. B
Page 40
To connect the detector to the gas supply:
1.
Connect one end of the plastic tubing to the nitrogen source.
Tip:
The nitrogen source can require an adapter to fit the plastic tubing.
2.
Connect the free end to the nitrogen fitting on the rear panel of the detector.
2.8
Starting up and shutting down the 2998 PDA detector
The detector startup procedure requires less than one minute. Following the procedure, allow the
detector to warm up for at least one hour before performing an analysis. Follow the procedures in
this section, to ensure reliable detector performance.
2.8.1
Starting the detector
To start the detector:
1.
In the instrument method, set the solvent delivery system or pump to deliver 10 mL of
HPLC-grade methanol or mobile phase.
Tip:
For details, refer to the Empower or MassLynx online Help.
Guidelines:
•
Use only thoroughly degassed HPLC-grade solvents. Gas in the mobile phase can
form bubbles in the flow cell, and cause the detector to fail the reference-energy
diagnostic test.
•
Ensure that the solvent is correctly composed and that it is of high quality and miscible
with any other solvents used in the system. Use filters in all solvent reservoirs, and
ensure that the volumes of solvents are sufficient for priming.
2.
Power-on the detector.
3.
Observe the lamp and power-indicator LEDs, on the front door of the detector which LEDs
change as follows:
•
During initialization, the power LED flashes green.
•
After the detector has successfully powered-on, the power and lamp LEDs show
steady green.
4.
Wait 1 hour for the detector to stabilize before acquiring data.
Warning:
To avoid explosion and fire, do not use gases that would allow the combustion of
combustible solvents. Always use inert gases.
Warning:
• Always observe Good Laboratory Practices when you use this equipment and when you
work with solvents and test solutions. Know the chemical and physical properties of the
solvents and test solutions you use. See the Material Safety Data Sheet for each solvent
and test solution in use.
• Using incompatible solvents can cause severe damage to the instrument and injury to
the operator.
Summary of Contents for 2998
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