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ProStar 335 Photodiode Array Detector Control Manual

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Introduction

General

This document describes the installation and configuration of
the Varian ProStar 335 Photodiode Array Detector driver. It
also explains how to build a control method for this driver, how
to start and view a running acquisition and what actions are
available during an analysis.

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Summary of Contents for ProStar 335

Page 1: ...Varian Analytical Instruments 2700 Mitchell Drive Walnut Creek CA 94598 1675 USA Varian Inc 2002 ProStar 335 Photodiode Array Detector Control Galaxie Driver Manual...

Page 2: ...1 Building a Method 24 Creating a New Method 24 Control Method Section for the Varian ProStar 335 Photodiode Array Detector 27 Signal Control 29 Purity Control 31 Analog Control 33 Peak Sensor Control...

Page 3: ......

Page 4: ...This document describes the installation and configuration of the Varian ProStar 335 Photodiode Array Detector driver It also explains how to build a control method for this driver how to start and v...

Page 5: ...ghts to install the driver The Varian drivers must be installed only on the server 1 When you insert the Galaxie CD into your CD drive the CD Browser will start automatically If it does not you can la...

Page 6: ...Next button read and accept the License Agreement and enter your driver Serial Number in the corresponding field The serial number is provided on your driver serial number card and is different from t...

Page 7: ...or Control Manual 6 Then press the Next button and click on Finish at the end of the installation 5 If you want to reinstall or upgrade your drivers you will need to run the setup steps 1 2 and 3 agai...

Page 8: ...ie Configuration Manager main menu and select Install Instrument Control Drivers The following screen containing the installed devices appears The button can be used for installing beta or pre release...

Page 9: ...also configured in the Varian Prostar 335 with the driver A BOOTP Server is used to send IP addresses to TCP IP devices The BOOTP Server lists Ethernet addresses along with the IP addresses that are t...

Page 10: ...by clicking on Delete the selected line by clicking on Delete all the lines by clicking on 3 Click on the button and the BOOTP server will automatically find the instruments which are requesting an IP...

Page 11: ...he mandatory fields by entering the IP address assigned to the selected instrument its Name or Description and the Network interface previously assigned to the instrument NOTE Do not check the Star 80...

Page 12: ...details about the requests sent by the instruments the answers of the BOOTP server or the errors 7 When all the instruments have been correctly configured click on OK or Apply to save the changes NOT...

Page 13: ...ol Server can be named as the owner The Sequence Engine Host option is reserved for a particular Galaxie Chromatography Data System application called External sequence and not provided in the standar...

Page 14: ...gives access to the following screen where all the available drivers are listed Select the Varian ProStar 335 Photodiode Array Detector driver 6 Click on the OK button The Create Edit System screen sh...

Page 15: ...on to remove the selected device from the list 7 When all the required devices are installed click on the OK button to accept the list A new screen appears and asks if the instrument devices should b...

Page 16: ...button corresponding to the Varian ProStar 335 driver and the configuration screen appears The toolbar allows you to easily scroll through the configuration windows to select the first configuration w...

Page 17: ...ector Control Manual 16 Communication Window In the IP Address field enter the IP address of the instrument Press the button to search the subnet for all connected Varian Prostar 335 instrument The fo...

Page 18: ...ption When this option is enable the Varian Prostar 335 will be started from a contact closure a will be able to receive a fault signal directly from another instrument Check the Enable Ready In box t...

Page 19: ...his option is active only with a 9x0 flow cell and scales the absorbance results to a 10 mm flow cell to allow absorbance comparison The scale factor applied to the absorbance is 10 9 If a prep flow c...

Page 20: ...address to the instrument To set a static IP address the user needs to communicate first with the instrument by Ethernet communication using BOOTP To set the Ethernet Parameters fill the following fi...

Page 21: ...ProStar 335 Photodiode Array Detector Control Manual 20 Click on the button to send the new Ethernet Parameters to the instrument Then reboot the instrument to apply the new Ethernet Parameters...

Page 22: ...n and click on Start 3 Go back to the System Configuration screen Properties wizard A graphic of the devices that comprise the system is displayed in the synopsis screen To configure the synopsis as t...

Page 23: ...y Data System in the Systems tab In this tab the Overview part will show the synopsis of the system with some parameters displayed wavelength signal etc depending on the installed devices For example...

Page 24: ...with one or more projects in order to start some acquisitions Remember to check the Control and View boxes in the Galaxie Configuration Manager Projects Properties For more details about the creation...

Page 25: ...of final results To initially set up a method it is not necessary to define all of the sub method sections however you must fill in the instrument control section prior to starting an acquisition 1 T...

Page 26: ...me in the first field In the Description field enter information concerning the method optional 5 Once these two fields have been completed click on the OK button The new method is created and is open...

Page 27: ...ProStar 335 Photodiode Array Detector Control Manual 26 NOTE For more details about the programming of each method section turn to the Galaxie Chromatography Data System User s Guide...

Page 28: ...Varian ProStar 335 Photodiode Array Detector Once the new method is created select the control section in the lower part of the browser The following screen appears Press the button to display the Sy...

Page 29: ...urity Analog Peak Sensor and Relay Click on the required sub section to define the corresponding parameters that will be used during the chromatographic run The toolbar allows you to easily scroll thr...

Page 30: ...ld This parameter specifies the time when the detector is running during one acquisition Enter the starting wavelength for spectral collection in the Min Wavelength field The entered value must be gre...

Page 31: ...te of the detector With increased bunch rates less disk space is required to store the data With decreased bunch rates more data is collected Bunch rate should be set appropriately for the narrowest p...

Page 32: ...Detector Control Manual 31 Purity Control Purity parameter group For a given wavelength range the Purity value is the average wavelength weighted by the square of the absorbance Ai i Ai Purity With L...

Page 33: ...nding wavelength for the purity wavelength range in the Max wavelength field The entered value must be less than maximum wavelength collected The following values are used to trigger the purity calcul...

Page 34: ...ll be chosen for the two analog outputs on the rear panel of the detector The same signal can be output from the two analog channels In the Analog X Peak Ticks scrolling list select enable or disable...

Page 35: ...S This affects only the recorder s output If Yes is selected in the Autozero field the detector s output is automatically zeroed This affects both the chromatogram display and the data acquired for pe...

Page 36: ...ially open Conversely if you command Open on activation the relay will be initially closed Enter the Duration time in the corresponding field The Duration is the length of time the relay is pulsed whe...

Page 37: ...resented by the total peak area of selected peaks and collect this sample in a single fraction When this function is activated peak start peak valley and peak end activate the Peak Sensor relay It is...

Page 38: ...slicing when a peak is detected Off Peak triggering turns off time slicing when any peak event is detected On Level triggering turns on time slicing when the signal is above a threshold value Off Leve...

Page 39: ...evel Sense or Pulse refer to the appendix Peak Sensor functions section at the end of this manual Relay Control This detector has four external event relays which may be time programmed Each step of t...

Page 40: ...ProStar 335 Photodiode Array Detector Control Manual 39 to delete a line in the table to clear the entire table...

Page 41: ...trument control method has been correctly programmed an analysis can be started The next section briefly describes how to start an acquisition Quick Start Single Acquisition 1 Press the button In the...

Page 42: ...omatography Data System the Injection Volume the Vial position and the Rack number if required must be entered Other parameters associated with each acquisition channel can be entered in the Channel X...

Page 43: ...samples and the sequence name 2 The sequence is displayed Complete the sequence information columns refer to the Galaxie Chromatography Data System User s Guide The Method Name the Run Name the Run I...

Page 44: ...tus Click on the button corresponding to the ProStar 335 driver and the Photodiode Array detector status screen will appear It contains up to five sub sections depending of the installed options Detec...

Page 45: ...nce Run Time End Time etc are shown with their current values depending on the control method program The light colors change depending on the detector state Lamp On Off Method Ready Not Ready Monitor...

Page 46: ...output attenuation to one of the sixteen available settings Attenuation is measured in Absorbance Units Full Scale AUFS This affects only the recorder s output Check or uncheck for each channel the Au...

Page 47: ...on the button to start the autozero of the detector Click on the button to upload the Error log or the Run log from the instrument Select with the dropdown list which log you want to upload About Win...

Page 48: ...n the instrument Overview Window Click on the button the Status Overview window is displayed and contains three tabs General displays the synopsis of the instrument where some current values can be re...

Page 49: ...or to start or stop the D2 lamp of the detector Click on or to start or stop the visible lamp of the detector Not ready contains the not ready information sent by the instrument Events contains the ev...

Page 50: ...unction can be used when you want to control the sample collector to collect the total sample represented by the total peak area of selected peaks and collect this sample in a single fraction When thi...

Page 51: ...button in the Galaxie Chromatography Data System and the following screen will appear Select the peak of interest and then Width 50 and the corresponding value is displayed in minute Based on your as...

Page 52: ...ak Sense function by time programming set the activation time at some point prior to the actual peak start The Peak Sense function will continue to detect peak start peak end and peak valley until it...

Page 53: ...to partition a selected peak into a number of segments At the completion of each segment the sample collector is advanced to the next sample vial by the Peak Sensor Relay The Time Slice function can b...

Page 54: ...ue When Time Slice triggered by Peak Sense or Level Sense is used it is best to time program the Time Slice On just before the peak and program Time Slice Off during peak elution Pulse Relay Function...

Page 55: ...ProStar 335 Photodiode Array Detector Control Manual 54 and overflow at about 3 minutes You may set the Pulse function to advance the sample collector at 2 75 minutes to the next sample receiver...

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