ColonyDoc-It Imaging Station
15
Lighting Tips:
There are four options for lighting the Petri dish.
Epi Lighting
options include
White
light and
Blue
light and
these sources light the Petri dish from the top.
Base Lighting
options include
Trans
and
Darkfield
and
these sources light the Petri dish from the bottom
Blue
light is used to excite GFP stained colonies.
A majority of samples prepared using the spread method and a transparent substrate that are bacterial,
yeast, or mold are best viewed using
Base Lighting > Trans
.
If there is excessive handwriting on the bottom of the Petri dish, membrane paper, or stickers, then use
Epi
Lighting > White
or
Base Lighting > Darkfield
.
Use
Base Lighting > Darkfield
when counting a pour plate.
Using the black plate on top of the transillumination surface in combination with
Epi Lighting
sources
provides contrast that improves colony counting results.
Preparing Samples:
When preparing samples, write on the bottom edge away from the center of the Petri dish to increase
counting accuracy.
Automatic Counting
Before proceeding through the automatic counting steps, ensure that an image of the Petri dish has been captured
and is ready for analysis. See instructions on
Quick Capture
in this manual for more information on how to capture
an image.
Select
Start Colony Count
along the
left side of the screen. A new
Colony
Count Type Selection
window
appears. Select
Automated Count
and
OK.
