ColonyDoc-It Imaging Station
13
Counting Colonies
Quick Capture
Place the Petri dish to be counted on the transillumination surface.
Select the appropriate lighting option for the sample. If unsure, turn the selector switch (located on the front of the
colony counter) to
Base Lighting > Trans
and remove the black plate (if present). The Trans lighting option will
apply to most applications.
If the colony counter is not already open, double click onto the
Doc-It Colony Counter icon
on the desktop.
Ensure that the
Camera Control
module is active. See the image of the screen below noted by the green circle.
The text must be black as shown. If the
Camera Control
is inactive (with grey text) proceed to the troubleshooting
section:
Inactive Camera Window
.
Select the Petri dish plate diameter from the
Camera Control
module. The choices are
6cm, 10cm,
and
15cm
.
Select the
smallest colony
size for this plate. The choices are
<1mm
and
1mm+
. If <1mm is selected the
smallest colony size is below 1mm. The count will be faster if 1mm+ is chosen.
Choose the
White Light
option from
Colony Lighting
. The
Dim
to
Bright
slider will be disabled unless using the
Fluorescent Colony Lighting
option. If counting fluorescent colonies go to the
Fluorescent Colony Counting
section of the manual.
