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Trace2O Metalyser HM5000, Instruction Manual

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Trace2O Metalyser HM5000 Instruction Manual Download Page 33

33

Metalyser HM5000 Benchtop 

Instruction Manual v1.0

www.trace2o.com

Square Wave

Square Wave voltammetry is also a derivative of linear sweep with a square wave 

super-imposed on the ramp but the centre of each square wave pulse is centred on 

the step potential. This method is the most effective against the effect of capacitive 

current and increases the signal to noise ratio giving lower detection limits.

Potential

Time

Summary of Contents for Metalyser HM5000

Page 1: ...METALYSER HM5000 BENCHTOP Laboratory Heavy Metals Analyser...

Page 2: ...6 7 INTRODUCING METAWARE 18 8 USING THE METAWARE 21 9 GRAPH TAB 25 10 SPREADSHEET TAB 28 11 MEASUREMENT TECHNIQUES 31 Linear Sweep 31 Differential Pulse 32 Square Wave 33 Cyclic Voltammetry 34 Table o...

Page 3: ...3 www trace2o com...

Page 4: ...consist of a deposition phase followed by a stripping phase for the element of interest This will produce a graphical voltammogram of the current output from the cell The voltammogram will feature pea...

Page 5: ...g cloth 1 x Bottle of polishing slurry 1 x Reference electrode fill solution bottle 1 x Bluetooth USB dongle 1 x USB lead 5 x Graduated sample analysis beaker 0 5 metres flexible N2 tubing 20cm 4mm I...

Page 6: ...6 www trace2o com Metalyser HM5000 Benchtop Instruction Manual v1 0 3 GETTING TO KNOW YOUR METALYSER On Off switch Sensor Module Sample Beaker Platform...

Page 7: ...7 Metalyser HM5000 Benchtop Instruction Manual v1 0 www trace2o com USB Connector 12V DC N2 Regulator N2 Inlet port...

Page 8: ...ug into a wall socket Nitrogen supply If a nitrogen supply is to be connected then there are several connection options depending on the supply available but the supply must have an external in line r...

Page 9: ...lt in nitrogen regulator on the back of the HM5000 by turning clockwise until it is finger tight Slowly open the external in line regulator or adjustable tap until there is a small level of audible ga...

Page 10: ...e R The working electrodes and reference electrode are supplied with protective rubber caps These are intended to protect the electrode surfaces They should be removed during analysis and replaced whe...

Page 11: ...the HM5000 instrument To disconnect the sensor module from the body of the instrument turn the sensor module cogwheel counter clockwise to unlock and carefully pull gently downwards To remove the sens...

Page 12: ...to the in line connector Connect plug A to socket B of the blue cable connector and tighten the locking ring Ensure that the working electrode socket is located towards the front of the sensor module...

Page 13: ...arrow on the black connector shroud with the arrow on the socket and gently push the electrode A quiet double click should be heard Gently pull on the top of the black holder of the electrode to ensu...

Page 14: ...ll reduce the sensitivity of the analysis The electrode should therefore be regularly polished to ensure a smooth mirror like surface using the supplied glass platen polishing cloth and polishing solu...

Page 15: ...se to prevent the reference electrode from drying out Add a few drops of deionised water to the cap before placing on the reference electrode Cleaning and maintaining the instrument sensor module and...

Page 16: ...to the CD drive Ensure that the HM5000 instrument is connected to a power supply using the 12V DC adapter Unscrew the blue protector cap on the rear of the HM5000 connect the waterproof USB cable to t...

Page 17: ...d turn on Double click the Bluetooth icon found in the system tray Depending on previous use of Bluetooth the configuration wizard may load Follow the on screen instructions Select Add device Metalyse...

Page 18: ...Select Programs Select Trace2o Click Trace2o Metaware Benchtop File Menu Control Panel Tools Menu Connection Status Table of Analyses Voltammogram Instrument ID Graph Tab Method Parameter Setup Tab S...

Page 19: ...g When a connection has been successfully established the connection status panel will read Connected and the serial number of the instrument will be displayed on the instrument ID panel If the Metaly...

Page 20: ...XXX where the X values correspond to the serial number of your instrument The connection status panel on the info pane will change from Disconnected to Connecting When a connection has been successfu...

Page 21: ...r file mxf file and select the method parameter of choice Click Open to open This will populate the Method Parameter Setup Tab For more information on method parameter setup see section X To load anot...

Page 22: ...he potentiostat To start the stirrer cick the Start button To stop the stirrer click the Stop button The stirrer has a variable speed setting 0 100 which is controlled by clicking and dragging the sli...

Page 23: ...atform panel of the info pane at the bottom of the Metaware window The panel will display either Down signifying that the platform is lowered Up signifying that the platform is raised or indicating ei...

Page 24: ...od Parameter Setup tab using the Deposition Time box The Deposition Time box will ALWAYS override the Tdep settings in the Method Parameter Setup tab For more information on appropriate usage of the d...

Page 25: ...hich then shows the X value peak potential and the Y value peak height of the peaks on the left hand side of the screen Right clicking the mouse whilst hovering over the graph will show these options...

Page 26: ...se and click calculate peak If a valid point is not found this function will be greyed out Move the mouse to the left hand side of the peak and left click the mouse The baseline will be drawn in and t...

Page 27: ...ime stamp in the format YYYYMMDD_HHMMSS The filename can be altered by saving the files with a different name using the File menu and choosing Save Data File As If a file is saved with a different nam...

Page 28: ...ear regression on the analysis data as well as allowing the creation of a calibration curve for use on further samples To create a spreadsheet right click on the drop down menu and select New Element...

Page 29: ...bove example three standard additions are performed each of 20ppb and the concentration is increased by 20ppb each time The peak heights can be recorded for each of the additions and up to three repea...

Page 30: ...min peak width for which the peak is recognised and the min peak height for which the peak is recognised Set the calibration temperature the temperature at which the calibration scans were carried ou...

Page 31: ...ves a brief explanation of these techniques Linear Sweep Linear sweep increases the potential from Ebegin to Eend at the scan rate in V s In any given metal analysis dependent on whether ASV or CSV is...

Page 32: ...tage steps The values of Epulse and Tpulse set the amplitude and time of the pulses The increased time at which the redox potential is held increases the current and provides a more sensitive techniqu...

Page 33: ...so a derivative of linear sweep with a square wave super imposed on the ramp but the centre of each square wave pulse is centred on the step potential This method is the most effective against the eff...

Page 34: ...d scans to Elow at which point it reverses and scans positive to Eend before reversing again and scanning back to the start potential NB Due to technical limitations of the instrument the cyclic volta...

Page 35: ...ential Pulse Square Wave Cyclic Voltammetry Description Edep V o o o Deposition potential Econd V o o o Conditioning potential Estep V o o o o Step potential Tpulse s o Time of pulse Eamp V o o Pulse...

Page 36: ...run The scans run in the following sequence 1 Conditioning Step 2 Deposition Step 3 Equilibration Step at Ebegin 4 Scan Step Once the scan is finished the graph will be drawn of potential vs current...

Page 37: ...nd potential of stripping step 2 00V to 2 00V Elow lowest potential of cyclic voltammetry sweep 2 00V to 2 00V Ehigh highest potential of cyclic voltammetry sweep 2 00V to 2 00V Tdep Deposition time 0...

Page 38: ...strument sensor module and electrodes Other polar protic solvents i e methanol water are also suitable for cleaning the instrument Acetone and other polar aprotic solvents DCM THF will damage the surf...

Page 39: ...r to the protective cap when storing the electrode Working electrode the electrode surface should be examined for cracks and imperfections that will affect analysis The glassy carbon should be regular...

Page 40: ...dealer for an annual service to keep the instrument in good condition For more information please contact Trace2o Firmware and software updates for the HM5000 are available free of charge via the Trac...

Page 41: ...ommunications USB Virtual Comm port 57600 Baud Bluetooth V2 0 2 4Ghz Potentiostat DC Potential range 2V DC Potential resolution 0 001V Output current range 10mA Input current range 1nA 1mA 7 ranges Re...

Page 42: ...ands Falkland Islands Ireland Malaysia Argentina Cape Verde Fiji Isle of Man Maldives Armenia Central African Republic Finland Israel Mali Australia Chad France Italy Malta Austria Channel Islands Fre...

Page 43: ...Arabia Anguilla Colombia Guatemala Okinawa South Korea Antigua Costa Rica Haiti Panama Suriname Aruba Cuba Honduras Peru 4 Taiwan Bahamas 2 Dominican Republic Mexico Philippines Trinidad Tobago Belize...

Page 44: ...11 Mains adapter HMA120021 USB cable HMA120002 Beaker 250mL HMA120003 Electrode wash bottle HMA120004 Graduated sample analysis beaker HMA120015 Micropipettor 200 1000 L HMA120009 Micropipettor 10 100...

Page 45: ...uffer sachets for 200 tests As HMC103200 M6 Buffer pack for 50 tests Mn HMC106050 M6 Buffer pack for 200 tests Mn HMC106200 Standards M1 Standard for Cd and Pb 50 tests at 20ppb per test HMC201050 M1...

Page 46: ...46 www trace2o com Metalyser HM5000 Benchtop Instruction Manual v1 0 NOTES...

Page 47: ...47 Metalyser HM5000 Benchtop Instruction Manual v1 0 www trace2o com NOTES...

Page 48: ...Technology Centre Unit 4 Transigo Gables Way Thatcham Berkshire RG19 4ZA United Kingdom Tel 44 0 1635 866772 Fax 44 0 1635 873509 info trace2o com www trace2o com...

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