standards, along with the slope, intercept and
correlation coefficient of the standard curve.
Measuring standards automatically (using Auto 6 or
Auto 3)
1.
Place the blank and standards in the correct
cell positions.
2.
When all the standards are correct, press
Measure Standards
to set up and run the
standards.
3.
Press
Measure Blank
to measure the blank. If
your instrument is equipped with a 6-Position
Cell Holder, it automatically moves to the B
position to measure the blank. When it
completes the measurement, it returns to its
previous cell position.
4. Press
Measure Standards
to measure the
standards. If your instrument is equipped with
a 6-Position Cell Holder, press the cell position
buttons on the keypad to re-position the cell
holder and measure the rest of the standards
manually.
When the instrument has measured all the
standards, the
Standards
screen appears,
showing the absorbance of each standards,
along with the slope, intercept and correlation
coefficient of the standard curve.
You can use this screen to:
•
Edit the standards (press
Edit Standards
)
•
Display a graph of the standard curve data
(press
View Graph
)
•
Save the standard curve (press
Save Test
)
•
Measure your samples (press
Run Test
)
Measuring protein samples
Measuring unknowns automatically (using Auto 6
or Auto 3)
1.
With the
Standards
screen displayed, place
the blank and unknowns in the correct cell
positions.
2.
Press
ENTER
to start the measurements. The
instrument automatically measures the blank
first, then measures the unknowns and
displays the absorbance and concentration of
each unknown.
Measuring unknowns manually (using Manual 6 or
Single Cell Positioner)
1.
With the
Standards
screen displayed, press
Run Test
.
2.
Place the blank and unknown in the correct cell
positions. If your instrument is equipped with a
6-Position Cell Holder, you can place up to five
samples in the cell holder.
3.
Press
Measure Blank
to measure the blank. If
your instrument is equipped with a 6-Position
Cell Holder, it automatically moves to the B
position to measure the blank. When it
completes the measurement, it returns to its
previous cell position.
4.
Press
Measure Sample
to start the
measurement. If your instrument is equipped
with a 6-Position Cell Holder, press the cell
position buttons on the keypad to re-position
the cell holder and measure the rest of the
unknowns manually.
Direct UV (280) and Direct UV (205)
The Direct UV methods determine protein
concentration based on absorbance at either 280
or 205nm. Refer to Appendix B for a description of
the parameters and Appendix C for the default
values.
To get started, move the arrow keys on the
BioMate tests
screen to highlight
Protein Tests
and press
ENTER
. Then the move the arrow keys
to highlight
Direct UV (280)
and press
ENTER
.
The
Direct UV (280)
parameter screen appears.
Note:
The following screens show the
parameters for the Direct UV tests at
280nm. For the Direct UV test at 205nm,
the wavelength is set to 205nm.
Using “Biotests” Software
2-9
BioMate 3 Operator’s Manual
Summary of Contents for 335904
Page 1: ...BioMate 3 Series Spectrophotometers Operator s Manual ...
Page 2: ......
Page 8: ......
Page 12: ...Thermo Electron Corporation iv ...
Page 60: ...Using General Tests Software Thermo Electron Corporation 3 28 ...
Page 70: ...Thermo Electron Corporation 5 4 Connecting Using Accessories ...
Page 74: ...Performing Maintenance Procedures Thermo Electron Corporation 6 4 ...
Page 80: ...Appendix B Parameters Thermo Electron Corporation B 4 ...
Page 88: ...Appendix C Calculations Thermo Electron Corporation C 8 ...
Page 92: ...Index Thermo Electron Corporation I 4 ...
Page 93: ......
Page 94: ...Item No 335904 10001 Rev G 06 03 Component No 335904 10063 Rev G 06 03 ...