Thermo 335904, Operator'S Manual, Page: 24 / 94

Measuring unknowns manually (using Manual 6 or
Single Cell Positioner)
1. With the DNA setup screen displayed, press
Run Test.
2. Place the blank and unknown in the correct cell
positions. If your instrument is equipped with a
6-Position Cell Holder, you can place up to five
samples in the cell holder.
3. Press Measure Blank to measure the blank. If
your instrument is equipped with a 6-Position
Cell Holder, it automatically moves to the B
position to measure the blank. When it
completes the measurement, it returns to its
previous cell position.
4. Press Measure Sample to start the
measurement. If your instrument is equipped
with a 6-Position Cell Holder, press the cell
position buttons on the keypad to re-position
the cell holder and measure the rest of the
unknowns manually.
DNA with Scan (260/280) and DNA with Scan
(260/230)
The DNA measurement with scan tests include two
test that function almost identically - the only
difference is in the wavelengths used for the
measurements. In both cases the scan is
measured from 225 to 325nm. One test measures
absorbance at 260 and 280nm, while the other
measures absorbance at 260 and 230nm. Refer to
Appendix B for a description of the parameters and
Appendix C for the default values.
To get started, with the BioMate Tests screen
displayed, move the arrow keys to highlight
Nucleic Acid Tests and press ENTER . A list of
nucleic acid test appears. Move the arrow keys to
highlight
DNA with Scan (260/280) . The DNA with
Scan (260/280) parameter screen appears.
Note: The following screens show the
parameters for the DNA (260/280) test.
For the DNA (260/230) test, Wavelength 2
is set to 230nm.
Note: If Cell Correction is ON , you must run the
Setup Correction program before you can
access the Run Test or Measure
Samples keys.
Setting up test parameters
1. With the DNA with Scan (260/280) or DNA
with Scan (260/230) screen displayed, use the
arrow keys to highlight the name of the
parameter you want to set.
2. When the parameters are set, you can press
Save Test to save the test or Run Test to
measure the blank and unknowns.
Collecting a baseline scan
Note: If your instrument is equipped with a 6-
Position Cell Holder, be sure to place the
blank in the B position. The instrument
always uses the B position to collect the
baseline.
1. With the DNA with Scan (260/280) or DNA
with Scan (260/230) screen displayed, press
Run Test. The DNA with Scan measurement
screen appears.
Using “Biotests” Software
Thermo Electron Corporation 2-4