Thermo 335904, Operator'S Manual, Page: 19 / 94

• Feed the paper straight into the paper entry
slot. The printer grabs the end of the paper and
pulls it in.
• In Basic Absorbance/%Transmittance (%T),
when the paper stops, press
ENTER to
continue advancing the paper until the paper
comes out of the paper exit slot.
• Pull out on the finger tabs on the paper roll
holders and secure the roll of paper onto the
paper roll holder.
Setting the utility parameters for the printer
If you wish to use a printer you can output to:
•
Internal printer.
• External RS232 printer.
To ensure that the spectrophotometer can output
information correctly to the printer, you need to
select the appropriate device.
•
Press the UTILITY key. The Utility screen
appears on the display.
• Press the arrow keys to highlight Printer and
press ENTER .
• Select the printer that you want to use and
press
ENTER until On appears.
• Press ESC to save the settings and return to
the Utility screen.
Selecting and positioning glassware
The wavelength range for different types of cells
varies depending on the manufacturer:
• Glass - From 320 to 360nm, up to 1100nm.
• Quartz - From 190 to 230nm, up to
1100nm.
• Disposable - Refer to manufacturer's
specifications and ensure that you work
within the recommended range.
Test tubes vs. cuvettes
• Square cuvettes that are carefully matched
(see “Correcting for cell variability” below) yield
very precise results. Matched test tubes, when
properly handled, can show as little as 1-2%
deviation between readings.
• The pathlength of test tubes is not as well
defined as in square cuvettes. However,
constructing a standard curve eliminates the
need for great accuracy in knowing the
pathlength, provided that the same pathlength
cell is used for all blanks, standards and
samples.
Other guidelines
• Be sure to position cuvettes and test tubes so
that the clear sides face the light beam. This
means that one clear side should face the front
of the instrument and the other clear side
should face the back of the instrument.
Note: Test tubes should always be placed in the
instrument in exactly the same orientation
in the light beam. A fiducial mark on the
test tube helps you orient the test tubes
consistently and correctly.
• When using small aperture cells:
•
Always use masked cells.
• Use the same cell (or cuvette) for your
blank and your samples.
Setting Up the Instrument
1-3 BioMate 3 Operator’s Manual