4
Tuning and Calibrating
Running Maintenance Tune
70
TSQ Quantum XLS Series User Guide
Thermo Scientific
Running Maintenance Tune
Use the maintenance tune procedure in EZ Tune to quickly increase the ion signal of the mass
spectrometer. The maintenance tune adjusts the lens L1 voltage and the lens L11 offset
voltage to maximize the ion signal for, for example, m/z 219 in FC-43 positive polarity mode.
At the end of the procedure you can save the optimized lens L1 voltage to a tune file and the
optimized lens L11 offset voltage to a calibration file.
shows a maintenance tune
report.
Figure 39.
Maintenance tune report, page 2
Optimizing Lens 1 for Q1MS
-45
-36
-27
-18
-9
0
Lens 1 Voltage (V)
0
20
40
60
80
100
R
e
la
t
iv
e
In
t
e
n
s
it
y
Feb 22, 2010
Previous Setting
Optimum Setting
Mass 218.99m/z : 117 % Improvement
Optimizing Lens 1 for Q3MS
-45
-36
-27
-18
-9
0
Lens 1 Voltage (V)
0
20
40
60
80
100
R
e
la
t
iv
e
In
t
e
n
s
it
y
Feb 22, 2010
Previous Setting
Optimum Setting
Mass 218.99m/z : 29 % Improvement
Optimizing Lens 1-1 for Q1MS
-15
-12
-10
-7
-5
-2
Lens 1-1 Voltage (V)
0
20
40
60
80
100
R
e
la
t
iv
e
In
t
e
n
s
it
y
Feb 22, 2010
Previous Setting
Optimum Setting
Mass 218.99m/z : 94 % Improvement
Optimizing Lens 1-1 for Q3MS
-15
-12
-10
-7
-5
-2
Lens 1-1 Voltage (V)
0
20
40
60
80
100
R
e
la
t
iv
e
In
t
e
n
s
it
y
Feb 22, 2010
Previous Setting
Optimum Setting
Mass 218.99m/z : 10 % Improvement
EIL1 DAC Scan #A: 19 Peak: -36.71; 1.36e+004
-45
-40
-35
-30
-25
-20
-15
-10
-5
0
Parameter Setting (Arb)
0
10
20
30
40
50
60
70
80
90
100
Rel
ati
v
e Ab
undanc
e
-36.71
-11.35
-17.07
-3.65
-30.00
-24.59
-9.37
-1.89
-21.53
-33.83
-27.03
-41.71
-38.92
L11 DAC Scan #A: 19 Peak: -2.69; 1.45e+004
-15
-14
-13
-12
-11
-10
-9
-8
-7
-6
-5
-4
-3
-2
-1
0
Parameter Setting (Arb)
0
10
20
30
40
50
60
70
80
90
100
Rel
ati
v
e Ab
undanc
e
-2.69
-10.16
-4.76
-7.61
-11.07
-8.34
-6.17
-3.76
-13.42
