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Thermo Scientific Surveyor UV Plus, Hardware Manual

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Thermo Scientific Surveyor UV Plus Hardware Manual Download Page 12

Contents

xii

Surveyor UV/Vis Plus Detector Hardware Manual

Thermo Scientific

LightPipe Flow Cell. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Removing the LightPipe Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Storing the LightPipe Flow Cell  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Installing the LightPipe Mounting Assembly . . . . . . . . . . . . . . . . . . . . . . . . . 30
Installing the LightPipe Flow Cell   . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Standard Analytical Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Removing the Standard Analytical Flow Cell Assembly . . . . . . . . . . . . . . . . . 33
Installing the Standard Analytical Flow Cell  . . . . . . . . . . . . . . . . . . . . . . . . . 35

Cleaning the Flow Cells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Cleaning the Flow Cell with Organic Solvents. . . . . . . . . . . . . . . . . . . . . . . . 36
Cleaning the Flow Cell with Nitric Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Lamp Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

Checking the Deuterium Lamp Usage Hours and Replacing the Lamp . . . . . 38
Replacing the Tungsten Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Chapter 4

Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45

Detector-Related Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Error Messages  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Chapter 5

Accessories and Replaceable Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51

Replaceable Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Optional Flow Cells for the UV/Vis Detector . . . . . . . . . . . . . . . . . . . . . . . . . . 52

Appendix A Surveyor Firmware Upgrade Utility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53

Index  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57

Summary of Contents for Surveyor UV Plus

Page 1: ...Thermo Surveyor UV Vis Plus Detector Hardware Manual 60053 97108 Revision F December 2013...

Page 2: ...hout notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purch...

Page 3: ...o Fisher Scientific or one of its authorized representatives Surveyor UV Vis Plus Detector EMC Directive 89 336 EEC amended by 92 31 EEC and 93 68 EEC EMC compliance has been evaluated by Underwriters...

Page 4: ...he instrument are not impaired Deviations from specified instructions on the proper use of the instrument include changes to the system and part replacement Accordingly order replacement parts from Th...

Page 5: ...Directives and the recyclers in your country WEEE Konformit t Dieses Produkt muss die EU Waste Electrical Electronic Equipment WEEE Richtlinie 2002 96 EC erf llen Das Produkt ist durch folgendes Symb...

Page 6: ...compagnies de recyclage dans chaque tat membre de l union europ enne et ce produit devrait tre collect ou recycl par celles ci Davantage d informations sur la conformit de Thermo Fisher Scientific ce...

Page 7: ...fahr durch entz ndbare Substanzen Beachten Sie die einschl gigen Vorsichtsma nahmen wenn Sie das System in Gegenwart von entz ndbaren Substanzen betreiben Peligro por sustancias inflamables Tenga much...

Page 8: ...away from this area Quetschgefahr Halten Sie Ihre H nde von diesem Bereich fern Puntos de pinzamiento Mantenga las manos apartadas de esta rea Risque de pincement loigner les mains de cette zone Heav...

Page 9: ...t surface Before touching allow any heated components to cool Flammable substances hazard Use care when operating the system in the presence of flammable substances Risk of eye injury Eye injury could...

Page 10: ...UV Sharp object Avoid physical contact with the object Pinch point Keep hands away from this area Heavy objects Never lift or move the instrument by yourself you can suffer personal injury or damage...

Page 11: ...Environmental Requirements 12 Installation Tools 12 Checking the Power Setting and Fuses 13 Making the Back Panel Connections 14 Connecting the UV Vis Detector to the Data System Computer 15 Setting...

Page 12: ...ly 33 Installing the Standard Analytical Flow Cell 35 Cleaning the Flow Cells 35 Cleaning the Flow Cell with Organic Solvents 36 Cleaning the Flow Cell with Nitric Acid 36 Lamp Maintenance 38 Checking...

Page 13: ...V Vis Plus Detector includes complete documentation In addition to this guide you can also access the following documents as PDF files from the data system computer Surveyor Plus Preinstallation Requi...

Page 14: ...N Alerts you to the presence of hot surfaces and to the potential injury that could occur were you to come in contact with a specific instrument area CAUTION Alerts you to the presence of an eye hazar...

Page 15: ...c com en support landing support html To copy manuals from the Internet Go to mssupport thermo com agree to the Terms and Conditions and then click Customer Manuals in the left margin of the window To...

Page 16: ......

Page 17: ...us Detector see Figure 1 is a member of the Surveyor Plus family of LC instruments This chapter provides an introduction to the Surveyor UV Vis Plus Detector Figure 1 Surveyor UV Vis Plus Detector Con...

Page 18: ...de a complete spectrum of ultraviolet UV and visible light the detector uses a deuterium lamp D2 for the UV range 190 to 365 nm and a tungsten lamp W for the visible range 366 to 800 nm The lamp cover...

Page 19: ...n page 7 You can order the UV Vis detector with one of these flow cell options Standard analytical flow cell with a 10 mm path length LightPipe flow cell with a 50 mm path length You can order other f...

Page 20: ...rom the detector replace the protective end caps for storage Figure 4 End of LightPipe flow cell showing the sensitive optical fiber Figure 5 LightPipe flow cell with protective end caps CAUTION Avoid...

Page 21: ...t sensitivity allow a warm up period of 1 5 hours The tungsten lamp typically requires only 15 minutes of warm up time The deuterium lamp has a lifetime of approximately 2000 hours and the tungsten la...

Page 22: ...ose CA 95134 U S A This device complies with FCC Rules Part 15 Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 This device must accept any i...

Page 23: ...eanings LED State Meaning Power Green The detector is on Comm Green Communication to the data system computer has been established Amber There is no communication with the data system Run Green The de...

Page 24: ...y deviation 5 up to 2 0 AU at 256 nm Scan rate 4 5 6 67 10 or 20 Hz user selectable Rise time 0 0 1 0 2 0 5 1 2 3 4 or 5 seconds user selectable Flow cell dimensions 10 mm 15 L standard analytical 50...

Page 25: ...LC system use the Installation Checklist on page 10 and follow the procedures in this chapter Contents Installation Checklist Unpacking and Inspecting the Instrument Placement and Environmental Requi...

Page 26: ...eference page Unpack and inspect your instrument 11 Provide the proper location and environment 12 Gather the necessary tools 12 Check the power settings and fuses 13 Make the back panel connections 1...

Page 27: ...kit Table 2 lists the contents of the accessory kit Carefully check to make sure you have received all the items listed on the packing list If any items are missing contact your Thermo Fisher Scientif...

Page 28: ...er circulating air sources A stable room temperature 0 5 C for applications requiring maximum detection sensitivity Clearance of at least 15 cm 6 in between the back panel of the UV Vis detector and a...

Page 29: ...ower entry module 3 Verify that the fuse holder contains the appropriate size fuses T2 5 A for 100 115 V or T1 25 A for 230 V 4 Ensure that the voltage selector displays the correct line voltage 5 If...

Page 30: ...transmits the start signal from the autosampler to the detector during an injection cycle as described in Making the Contact Closure Connections on page 16 If you are using an autosampler that is not...

Page 31: ...rrives with the unit ID preset using the two rotary switches located on the back panel Figure 11 shows the rotary switches preset to a value of 01 The range of values for the unit ID is 01 to 99 The v...

Page 32: ...can identify the detector connector by the DET tag on its adjacent cable as shown in Figure 12 The newer version has seven combicon connectors with three connectors labeled DETECTOR as shown in Figur...

Page 33: ...identified by the blue sticker with the text LC PUMP to the 8 pin receptacle on the back panel of the pump Look for a blue label with the text LC PUMP above the receptacle To connect the Surveyor MS...

Page 34: ...lug a connector labeled PUMP into the 8 pin socket on the back panel of the pump To connect the Surveyor MS Pump Plus do the following Connect the end of the adapter cable labeled PUMP to one of the P...

Page 35: ...S M S DETECTOR DETECTOR DETECTOR DETECTOR PUMP PUMP MS Detector Autosampler Pump Detector Detector Interconnect cable Solvent platform Autosampler back panel Surveyor LC Pump Plus back panel Detector...

Page 36: ...UV Vis detector CHA 10 mV CHA 100 mV CHA 1 V CHB 10 mV CHB 100 mV CHB 1 V and a single ground per channel GND A and GND B To make each electrical connection 1 Insert the end of the wire into the appro...

Page 37: ...closure connection do the following If your LC system contains a Surveyor Autosampler Plus use the 7 connector interconnect cable If your LC system contains a manual injector or another type of autosa...

Page 38: ...ll the detector arrives with the flow cell installed For more information about the standard analytical flow cell see Standard Analytical Flow Cell on page 33 When you order the detector with the Ligh...

Page 39: ...er source 3 Turn the power on by pushing the power button in to engage it Table 5 Optional flow cells for the UV Vis detector Flow cell type Path length mm Volume L Part number Kel F bio cell 6 9 0 95...

Page 40: ...ds the LEDs on the front of the detector turn solid green If one or more LEDs flash green or amber or turn solid amber there might be a problem The COMM LED remains amber until the software is started...

Page 41: ...s If you have any questions on proper maintenance or would like to arrange for a preventive maintenance program contact your Thermo Fisher Scientific field service engineer To maintain your UV Vis det...

Page 42: ...a possible contamination problem flush the flow cell with water or methanol If this does not remove the contamination clean the flow cell with a dilute solution of nitric acid followed by a water rins...

Page 43: ...cloth moistened with water only to remove dirt or stains LightPipe Flow Cell To remove or install the LightPipe flow cell follow these procedures Removing the LightPipe Flow Cell Storing the LightPipe...

Page 44: ...ipe cover See Figure 19 Figure 19 Front panel of the UV Vis detector with the LightPipe and lamp covers 3 Using the 3 32 in hex wrench supplied loosen and remove the two photodiode mount retention scr...

Page 45: ...of the way inside the detector housing or on the benchtop 5 Loosen the thumbscrew holding the retention block to the LightPipe retention bar See Figure 21 Figure 21 Retention block thumbscrew 6 Lift...

Page 46: ...e mounting assembly To install the LightPipe mounting assembly 1 Turn the detector off unplug the detector from line power and allow sufficient time for the lamps to cool before you remove the lamp co...

Page 47: ...gure 20 on page 28 b Carefully pull the photodiode mount straight back and off the LightPipe mounting assembly Place the photodiode mount out of the way inside the detector housing or on the benchtop...

Page 48: ...e mounting assembly Be careful not to touch the photodiode while manipulating the photodiode mount b Using the 3 32 in hex wrench supplied screw in and tighten the two photodiode mount retention screw...

Page 49: ...alytical flow cell 1 Turn the detector off and unplug the detector from line power 2 Open the front doors of the detector The flow cell assembly is located behind the flow cell cover 3 Remove the flow...

Page 50: ...p See Figure 27 Figure 27 Photodiode mount resting on the detector s drip tray 6 To free the tubing from the UV Vis detector remove the thumbscrew that holds the tubing retainer bracket to the UV Vis...

Page 51: ...ew just enough to hold the tubing in place 7 Guide the tubing through the slots in the flow cell cover and then securing the cover with the flow cell cover mounting screw 8 Connect the power cord to t...

Page 52: ...ntaminated after flushing it with organic solvents remove the flow cell from the detector and clean it with nitric acid IMPORTANT Ensure that the cleaning solvents you plan to use are miscible with th...

Page 53: ...ed in the chromatographic system and the cleaning solution 5 Prepare a 20 vol vol solution of nitric acid in HPLC grade water 6 Pump the nitric acid solution through the flow cell 7 To remove all trac...

Page 54: ...emblies to make them easy to install and align To replace the detector s lamps follow these procedures Checking the Deuterium Lamp Usage Hours and Replacing the Lamp Replacing the Tungsten Lamp Checki...

Page 55: ...r off to expose the lamp assemblies 5 To read the chronometer check the position of the electrolyte gap in the mercury tube against the graduated background see Figure 29 and Figure 30 Figure 29 Chron...

Page 56: ...to twist the connector Figure 31 Deuterium lamp parts 3 Remove the two thumbscrews with standoffs that hold the lamp assembly in place and pull the assembly straight out Note Two mercury columns separ...

Page 57: ...ent pin see Figure 32 Figure 32 Deuterium lamp assembly alignment pin 4 Securely fasten the assembly in place with the two thumbscrews and standoffs 5 Connect the lamp lead to the upper terminal in th...

Page 58: ...pose the lamp assemblies 5 Gently unplug the tungsten lamp lead from the detector see Figure 31 Take care not to twist the connector Figure 33 Tungsten lamp assembly 6 Loosen and remove the thumbscrew...

Page 59: ...tall a new tungsten lamp 1 Remove the used tungsten lamp as described in Removing the Tungsten Lamp on page 42 2 Hold the lamp assembly so that the leads are at the top Slide the assembly onto the two...

Page 60: ...al Thermo Scientific 3 Securely fasten the assembly in place with the thumbscrew and standoff 4 Replace the lamp housing and secure it with the screw 5 Close the front doors of the detector 6 Plug the...

Page 61: ...blems Error Messages Table 7 Detector related problems Sheet 1 of 3 Symptom Cause Remedy Spikes on baseline Continuous gas bubbles in the flow cell Degas the mobile phase Connect a backpressure device...

Page 62: ...Clean the dirty component See Cleaning the Flow Cells on page 35 Integrator input and output voltage not matching Verify that the integrator is connected to the appropriate analog output connections...

Page 63: ...lection Poor connection at flow cell inlet Check the end of the inlet tubing for a clean flat surface free of obstructions Detector not powering up Tripped circuit breaker at power outlet Reset the ci...

Page 64: ...reset 2002 System error bus address 2004 Lamp case open 2011 Sample light is too high 2012 Low light on D2 lamp 2013 Low light on W lamp 2014 Volatile RAM test failed 2015 Non Volatile RAM test faile...

Page 65: ...amp off option 2212 Invalid program time 2213 Invalid wavelength for program 2215 Invalid program type 2216 Invalid output range 2217 Invalid rise time filter 2218 Invalid auto zero time 2219 Invalid...

Page 66: ......

Page 67: ...p 6040 0167 Cable tungsten lamp 6040 0168 Cable display LEDs F5025 010 Cover lamps 60053 40042 Cover LightPipe 60053 40041 Cuvette holder assembly 9550 0263S Door right assembly 60053 60068 Door left...

Page 68: ...order these flow cells and flow cell covers from Thermo Fisher Scientific Kel F bio cell 9550 0103S 3 mm semi prep 9550 0101S 6 mm analytical 9550 0100S High pressure Microbore 9550 0150 01S Titanium...

Page 69: ...e files are located in the following folder drive Xcalibur system Surveyor Firmware 2 Set the Unit ID on the detector s back panel to the maintenance setting as follows a Ensure that the UV Vis power...

Page 70: ...rveyor UV Vis option selected b Click Connect The status display at the bottom of the Surveyor Firmware Upgrade Utility window indicates that a connection has been made See Figure 37 CAUTION Do not in...

Page 71: ...The file name appears in the ROM box See Figure 37 b Click Browse to the right of the APP1 box and then select the APP1_UVVis_1 10 bin file The file name appears in the App1 box See Figure 37 8 Click...

Page 72: ...rade Utility The connection with the Surveyor UV Vis Plus Detector automatically terminates 10 Reset the Unit ID on the back panel of your detector for normal operation as follows a Turn off the power...

Page 73: ...r 20 column bleed 46 compliance FCC iii regulatory iii WEEE v connections analog output 20 Ethernet 15 remote communications inputs 21 contacting us xv D deuterium lamp description 5 installing 41 lif...

Page 74: ...d analytical flow cell removing 33 maintenance tools 27 N nitric acid safety precautions 37 noise causes of 46 O operating environment humidity 8 temperature 8 optical bench figure 2 optical fiber fig...

Page 75: ...ription 5 installing 43 lifetime 5 38 removing 42 U uninterruptible power supply UPS 45 unit ID setting 15 unpacking your detector 11 V voltage ac line problems 45 setting changing 13 voltage selector...

Page 76: ......

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