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Maintenance and Care

Autoclaving the rotors

7-6

Sorvall RC 3BP Plus

Thermo Scientific

Autoclaving the rotors

1. Before autoclaving clean rotor and accessories as described above.

2. Place the rotor on a flat surface.

•

Rotors and adapter can be autoclaved at 121° C.

•

The maximum permissible autoclave cycle is 20 minutes at 121° C.

Clean the rotor before autoclaving and rinse it with distilled water. Remove all accessories (tubes,
adapters). Place the rotor on a flat surface.

Service of Thermo Fisher Scientific

Thermo Fisher Scientific recommends having the centrifuge and accessories serviced once a year by an
authorized service technichian. The service technicians check the following:

•

the electrical equipment

•

the suitability of the set-up site

•

the lock and the safety system

•

the rotor

•

the fixation of the rotor and the drive shaft

Thermo Fisher Scientific offers inspection and service contracts for this work.

Note

No chemical additives are permitted in the steam.

CAUTION

Never exceed the permitted temperature and duration when autoclaving.

If the rotor shows signs of corrosion or wear, it must be replaced.

Summary of Contents for RC 3BP Plus

Page 1: ...50113587 6 04 2013 Thermo Scientific Sorvall RC 3BP Plus Instruction Manual Visit us online to register your warranty www thermoscientific com labwarranty ...

Page 2: ......

Page 3: ...Thermo Scientific Sorvall RC 3BP Plus Instruction Manual 50113587 6 04 2013 ...

Page 4: ...copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous in...

Page 5: ...ereinstimmung mit allen einschlägigen Bestimmungen der Richtlinie 200 4 1 0 8 EG ü be r e le kt rom ag n eti sc h e Ve rträg I i c h ke it The machinery is in accordance with all relevant terms of directives for electromagnetic compatibility 2004n08 EC Die Schutzziele der Niederspannungsrichtlinie 2006 95 EG werden eingehalten The protection goals of the directive for low voltage 2006 95 EC are me...

Page 6: ...eements with recycling and disposal companies in all EU Member States for the recycling and disposal of this device For information on recycling and disposal companies in Germany and on the products of Thermo Fisher Scientific which fall under the RoHS Directive Restriction of the use of certain hazardous substances in electrical and electronic equipment please visit the website www thermoscientif...

Page 7: ...pter 2 Before use 2 1 Before Setting up 2 2 Location 2 2 Setting up 2 2 Transporting the Centrifuge 2 5 Mains Connection 2 5 Other Connections 2 5 Storage 2 6 Chapter 3 Control Panel 3 1 Control Panel 3 2 Numeric Keypad 3 2 Run Display 3 2 Set Display 3 3 Primary Function Keys 3 3 Primary Functions 3 4 Keypad Lock 3 6 Chapter 4 Operation 4 1 Switch on Centrifuge 4 2 Open door 4 2 Close door 4 2 Ro...

Page 8: ... Inspection 6 3 Chapter 7 Maintenance and Care 7 1 Cleaning Intervals 7 2 Cleaning 7 2 Disinfection 7 4 Decontamination 7 5 Autoclaving the rotors 7 6 Service of Thermo Fisher Scientific 7 6 Chapter 8 Troubleshooting 8 1 Mechanical Emergency Door Release 8 2 Circuit Breakers 8 3 Failure message 8 3 Chemical Compatibility Chart A 1 Warranty B 1 Contact Information C 1 ...

Page 9: ...s instruction manual will result in the expiration of the sellers warranty Scope of Supply If any parts are missing please contact your nearest Thermo Fisher Scientific representative This symbol refers to general hazards CAUTION means that material damage could occur WARNING means that injuries or material damage or contamination could occur This symbol refers to a rotor failure 3 This symbol ref...

Page 10: ...for separating materials of different density or particle size suspended in a liquid Maximum sample density at maximum speed 1 2 Accident Prevention g cm 3 Prerequisite for the safe operation of the centrifuge is a work environment in compliance with standards directives and trade association safety regulations and proper instruction of the user The safety regulations contain the following basic r...

Page 11: ...tors which show any signs of corrosion and or cracks Do not touch the mechanical components of the rotor and do not make any changes to the mechanical components Use only with rotors which have been properly installed Follow the instructions in section Rotor Installation on page 4 3 Use only with rotors which have been loaded properly Follow the instructions given in the rotor manual Never overloa...

Page 12: ...entrifuge in an explosion prone environment Do not centrifuge inflammable substances Do not centrifuge toxic or radioactive materials or any pathogenic micro organisms without suitable safety precautions When centrifuging microbiological samples from the Risk Group II according to the Bio safety Manual of the World Health Organization WHO aerosol tight biological seals have to be used For material...

Page 13: ...nd Description Contents Characteristics of the Thermo Scientific Sorvall RC 3BP Plus on page 1 2 Technical Data on page 1 3 Directives Standards and Guidelines on page 1 4 Functions and Features on page 1 4 Mains Supply on page 1 5 Rotor Selection on page 1 5 ...

Page 14: ...d braking curves You can choose between the display of speed and RCF or the entry mode These settings can be changed even while the centrifuge is running The Sorvall RC 3BP Plus is equipped with various safety features Housing rotor chamber baseframe and guard ring are made of high strength high quality steel The door is equipped with a view port and a lock The door of the centrifuge can only be o...

Page 15: ... 2 2 2 Heat dissipation 230 V 208 V 400 V 5 2 kW 4 5 kW 5 8 kW 17700 BTU h 15300 BTU h 19700 BTU h IP 20 20 20 Running time unlimited unlimited unlimited Maximum speed nmax 5000 rpm 5000 rpm 5000 rpm Minimum speed nmin 300 rpm 300 rpm 300 rpm Minimum sample temperature 4 C bei 4700 rpm 4 C bei 4550 rpm 4 C bei 4700 rpm Maximum RCF value at nmax 7277 xg 7277 xg 7277 xg Maximum kinetic energy 153 kJ...

Page 16: ...Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area i...

Page 17: ... rotors and the corresponding adapters and reduction sleeves for various commercially available containers can be found in the corresponding rotor operating manuals For more information visit our website at www thermoscientific com centrifuge Table 1 4 Electrical connection data Article number Mains voltage Frequency Rated current Powercon sumption Equipment fuse Building fuse Mains plug 75007530 ...

Page 18: ...C 3BP Plus 2 1 2 Before use Contents Before Setting up on page 2 2 Location on page 2 2 Setting up on page 2 2 Transporting the Centrifuge on page 2 5 Mains Connection on page 2 5 Other Connections on page 2 5 Storage on page 2 6 ...

Page 19: ...must be kept out of the safety zone while centrifuging The location must be stable and free of resonance The location must be suitable for horizontal setup of the centrifuge The centrifuge should not be exposed to heat and strong sunlight The set up location must be well ventilated at all times Setting up 1 Place the pallet with the centrifuge so that you have at least 2 m room in front of the pal...

Page 20: ...nder the studs and screw them down 6 Remove the 4 screws that fix the centrifuge on the pallet The heads are upside down in the pallet 7 Remove the bolt spacers 8 Screw the studs with the safety brackets entirely up Otherwise they might get stuck when rolling the centrifuge off the pallet 9 Remove the rubber feet Rails Studs Safety brackets ...

Page 21: ...n the centrifuge is leveled 20 Be sure that the 4 locking stabilizers stand safe and do not jolt The final assembly of the safety brackets is carried out after leveling 21 Tighten the locking nuts of the locking stabilizers firmly Mount the safety brackets by means of an 8 mm open end wrench on the stabilizers 22 Optional To be certain the centrifuge is working properly refer to the Customer Contr...

Page 22: ... of the front cabinet panel press 0 2 Check whether the cable complies with the safety standards of your country 3 Make sure that the voltage and frequency correspond to the figures on the rating plate 4 Plug the centrifuge into grounded electrical sockets only Other Connections Figure 2 1 Connetors to external devises Note The packaging is a one way packaging Assign a logistic company for the tra...

Page 23: ... Scientific Storage Before storing the centrifuge and the accessories it must be cleaned and if necessary desinfected and decontaminated Store the centrifuge in a clean dust free location Be sure to place the centrifuge on its feet Avoid direct sunlight ...

Page 24: ...C 3BP Plus 3 1 3 Control Panel Contents Control Panel on page 3 2 Numeric Keypad on page 3 2 Run Display on page 3 2 Set Display on page 3 3 Primary Function Keys on page 3 3 Primary Functions on page 3 4 Keypad Lock on page 3 6 ...

Page 25: ... door only opens when the rotor is not spinning The message OPEN appears in the run display Press the key to confirm a selection or to step through the menu Use the numeric keypad to enter values into the set display The removes input values or messages from the set display The toggles between positive and negative temperature values Run Display The run display shows the actual values during a run...

Page 26: ...start of the run Set Display The set display is used to input and display basic run parameters to select options or other advanced features and to display advisory messages The set display shows the currently selected rotor values for speed temperature time and the number of the program if a recalled program is used After pressing the key to access advanced feature options pressing the key again e...

Page 27: ...ets carriers with different maximum radii each configuration will be listed for selection to calculate the different RCF The key is not active when a run is in progress SPEED Press the key to specify a different rotor speed rpm or RCF Speed is selectable from 300 to 4700 rpm depending on the specified rotor The equivalent RCF value is calculated at the maximum radius of the specified rotor Adapter...

Page 28: ...f 99 59 In the run display minutes and seconds begin to count down The run terminates when the timer reaches zero Now the deceleration of the rotor begins HOLD Press the key to start a continuous run The run terminates when you press the key During a continuous run the set dispaly shows the message HOLD and ELAPSED The time passed since the key has been pressed is shown in the run display The time...

Page 29: ...o When you press the key after the key the remaining runtime equals the preset run time Between runs the run display shows the previous run duration values Press the key to see the MIN SEC value in the run display Press the key to see the MIN SEC value in the run display after the rotor is in standstill Press the key to see the ACE value in the run display after the rotor is in standstill MENU and...

Page 30: ...s may only perform runs using run parameters recalled from program memory only the numeric keypad and the keys and are active Use of the KEYPAD LOCK may offer a considerable advantage improving process control by reducing the possibility for operator error as well as restricting unauthorized run parameter or program memory changes ...

Page 31: ...peration Contents Switch on Centrifuge on page 4 2 Rotor Installation on page 4 3 Normal Operation on page 4 3 Centrifugation on page 4 5 Programmed Operation on page 4 6 Temperature Control on page 4 7 Rotor Temperature Equilibration on page 4 7 ...

Page 32: ...or 1 Press the key Close door Close the door by pressing down on it lightly in the middle or on both sides of it Two locks close the door completely The door will close automatically Note Make sure the centrifuge is properly installed before turning it ON for the first time see Setting up on page 2 2 Note If any LED segments do not light note which ones and inform Customer Service Continued operat...

Page 33: ...ts or residue from the chamber 2 Follow the instructions given in the rotor manual in order to install load and balance the rotor 3 Close the centrifuge door 4 Make sure the rotor matches the rotor in the SET DISPLAY 5 Press the key repeatedly until the name of the installed rotor appears Normal Operation Preselecting Speed RCF 1 Press the key The display shows the RPM or the RCF value depending o...

Page 34: ... with the key Preselecting the temperature You can preselect temperatures between 10 C and 40 C 1 Press the key in order to enter the temperature Note If an extremely low RCF value has been selected it will be corrected automatically if the resulting speed is less than 300 rpm This is because 300 rpm is the lowest selectable speed Explanation of RCF Value The relative centrifugal force is given as...

Page 35: ...ding in the rotor instruction manual For information on troubleshooting see section Failure message on page 8 3 Stopping the centrifugation program Set run time Usually the run time is preset and you only have to wait until the centrifuge stops automatically when the preset time limit expires As soon as the speed drops to zero the message END will appear in the SPEED field By pressing the key you ...

Page 36: ...efore overwriting press the key If you press the key you will have to go back and re establish the run parameters before you can store them to memory Performing a Programmed Run Read the Safety Information Page at the front of this manual 1 Press the key The SET DISPLAY will change to display a program number flashing in the PROG field 2 Input the desired program number then press key The SET DISP...

Page 37: ...erature as the sample and the required SET temperature When the centrifuge is used to run large volumes of sample for short amounts of time the temperature control is less important During a very short run it is difficult to significantly change sample temperature Rotor Temperature Equilibration To equilibrate the rotor temperature in the centrifuge chamber 1 Install the rotor in the rotor chamber...

Page 38: ... enter the desired temperature 8 Press the to begin temperature equilibration 9 The centrifuge will alert you that a sample overtemperature condition exists and that pre cooling is taking place Press the key 10 Monitor progress of the value in the RUN TEMPERATURE display Note the time when the displayed RUN temperature reaches the SET temperature From the time it does allow the run to continue for...

Page 39: ...RC 3BP Plus 5 1 5 Advanced Features Contents QC RUN on page 5 4 SLOW START on page 5 4 SLOW STOP on page 5 5 BRAKE OFF on page 5 5 CHANGE OVERTEMPERATURE LIMIT on page 5 5 STEP RUN on page 5 6 SAVE TO PROGRAM MEMORY on page 5 7 ...

Page 40: ...nding indicator will be lit If changes to advanced feature selection are required press the key and then the key until selection screen of the particular feature appears The key accesses the advanced feature options and the program memory Press the key to enter the rotor selection Press the key to enter the advanced features where you can check and change parameters as well as select the advanced ...

Page 41: ...ic Sorvall RC 3BP Plus 5 3 Figure 5 1 Advanced Feature Option Screens 1 MENU MENU 0 or ENTER ENTER START ENTER ENTER ENTER ENTER ENTER 1 1 1 1 1 1 MENU MENU MENU MENU MENU MENU 0 or ENTER 0 or ENTER 0 or ENTER 0 or ENTER 0 or ENTER 0 or ENTER Set Display ...

Page 42: ...a QC RUN we recommend the following Save runs to program memory Sort first by temperature highest first then if there is more than one run at a single temperature by speed lowest first Start with program 1 The programs below are listed in correct order for using QC RUN program values shown are for example purposes only actual set parameters will vary To select the QC RUN feature 1 Press the key 2 ...

Page 43: ...RAKE OFF transition speed is set independently of set run speed and is not affected by change to set run speed If the transition speed is set higher than the set run speed at run termination the centrifuge will coast to a stop from set speed After BRAKE OFF selections are completed CHANGE OVERTEMPERATURE LIMIT will appear in the set display CHANGE OVERTEMPERATURE LIMIT CHANGE OVERTEMPERATURE LIMIT...

Page 44: ...lay STEP RUN STEP RUN allows the linking together of up to 3 sets of programmed run parameters to automatically perform step run protocols If the desired parameters have not been entered into memory you must save each desired set of parameters to memory before continuing with step run creation If programs are used within a step run that have not been defined before a warning message will be displa...

Page 45: ... option selections and settings excluding rotor name and overtemperature limit will also be saved to program memory To eliminate inadvertent loss of existing programs the save run sequence alerts users before overwriting The key allows browsing through existing programs for selection or reference To save a run all run parameter selections should be made before selecting this option After SAVE RUN ...

Page 46: ...Thermo Scientific Sorvall RC 3BP Plus 6 1 6 Control Inspection Contents QC RUN on page 6 2 Speed Controls on page 6 3 Timer Controls on page 6 3 Temperature Controls on page 6 4 ...

Page 47: ... rotor No program should use HOLD EXAMPLE The programs below are listed in correct order for using QC RUN program values shown are for example purposes only actual set parameters will vary While the QC RUN is in progress the parameters of the program that is running will be in the SET DISPLAY During a QC RUN the centrifuge checks if the preset speed and temperature can be reached In order to save ...

Page 48: ...reach the specified set speed Wait an additional 5 minutes for speed to stabilize 9 Check rotor speed through the viewing port in the centrifuge door using a strobe tachometer in rpm following instructions supplied with the tachometer The tachometer should indicate rpm equal to the SET value 20 rpm or 2000 rpm and below rpm above 2000 rpm 10 If necessary repeat this procedure at other speeds that ...

Page 49: ...Enter the program parameters Choose the protocol with the longest run time 2 Rotor chamber rotor and test fluid must have the desired sample temperature 3 Prepare two equal loads of test fluid similar in volume to normal run samples equilibrated to the desired sample temperature Check the sample temperature using a calibrated thermometer 4 Install the rotor in the rotor chamber 5 Load and balance ...

Page 50: ...BP Plus 7 1 7 Maintenance and Care Contents Cleaning Intervals on page 7 2 Cleaning on page 7 2 Disinfection on page 7 4 Decontamination on page 7 5 Autoclaving the rotors on page 7 6 Service of Thermo Fisher Scientific on page 7 6 ...

Page 51: ...se only disinfectants with a pH of 6 8 Dry aluminum parts off with a soft cloth After cleaning treat the entire surface of aluminum parts with corrosion protection oil 70009824 Also treat the cavities with oil Store the aluminum parts at room temperature or in a cold storage room with the cavities pointing down Maintenance Recommended Interval Clean rotor chamber Once per week or when polluted Cle...

Page 52: ...on oil 70009824 Also treat the cavities with oil Tread the bolt of the swing out rotor with bolt grease 75003786 Defrost rotor chamber After some applications there might be ice in the rotor chamber 1 Install a rotor and close the centrifuge 2 Set run parameters to SPEED 2500 rpm TIME 5 00 longer for heavy frost buildup TEMP 30 3 Press the key Clean the rotor chamber as described above CAUTION Obs...

Page 53: ... and then rub down 9 Dispose of the disinfectant according to the applicable guidelines 10 Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 C After cleaning treat the entire surface of aluminum parts with corrosion protection oil 70009824 Also treat the cavities with oil Tread the bolt of the swing out rotor with bolt grease ...

Page 54: ...9 Dispose of the decontamination solution according to the applicable guidelines 10 Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 C After cleaning treat the entire surface of aluminum parts with corrosion protection oil 70009824 Also treat the cavities with oil Tread the bolt of the swing out rotor with bolt grease 7500378...

Page 55: ... a flat surface Service of Thermo Fisher Scientific Thermo Fisher Scientific recommends having the centrifuge and accessories serviced once a year by an authorized service technichian The service technicians check the following the electrical equipment the suitability of the set up site the lock and the safety system the rotor the fixation of the rotor and the drive shaft Thermo Fisher Scientific ...

Page 56: ...Thermo Scientific Sorvall RC 3BP Plus 8 1 8 Troubleshooting Contents Mechanical Emergency Door Release on page 8 2 Circuit Breakers on page 8 3 Failure message on page 8 3 ...

Page 57: ... The braking process lasts much longer than usual Proceed as follows 1 Make sure the rotor has stopped view port in the door 2 Pull out the power supply plug 3 Unlock the front door by means of square box wrench 4 Open the front door 5 Pull down both levers on the right hand side The door lock is released mechanically The door will open and the samples can be removed Figure 8 1 Emergency door rele...

Page 58: ...ck the load placed in the rotor Check that the rotor cross bolts are well greased Restart the centrifuge If an error message appears again inform Customer Service CRITICAL OVERTEMP The centrifuge cannot be operated The run does not start or the centrifuge runs down without being braked The run will not be interrupted Turn off the centrifuge Pull out the power supply plug Inform Customer Service SA...

Page 59: ...CH FAILURE The centrifuge cannot be operated The centrifuge runs down without being braked The door lock opened during a run Turn off the centrifuge Pull out the power supply plug Inform Customer Service DOOR LOCK SWITCH SHORTED Centrifuge does not open The power supply for the door lock is broken The emergency lid release enables you to retrieve your samples Turn off the centrifuge Pull out the p...

Page 60: ...e speed is more than 200 rpm above the rotor s maximum rated speed The emergency lid release enables you to retrieve your samples Turn off the centrifuge Pull out the power supply plug Inform Customer Service MEMORYDATANOT SUITABLE FOR INSTRUMENT The centrifuge cannot be operated The run does not start or the centrifuge runs down without being braked The centrifuge detected during self testing tha...

Page 61: ...e enables you to retrieve your samples Pull out the power supply plug Inform Customer Service FREQUENCY CONVERTER VOLTAGE TO HIGH The centrifuge cannot be operated The run does not start or the centrifuge runs down without being braked The line voltage exceeds the tolerance or failure of the braking resistance Restart the centrifuge If an error message appears again turn off the centrifuge The eme...

Page 62: ...S Ammonium Carbonate M S U S S S S S S S S S S S U U S S S S S S M S S S Ammonium Hydroxide 10 U U S U S S M S S S S S S U M S S S S S S S S S M S Ammoniumhydroxid 28 U U S U S U M S S S S S U S U M S S S S S S S S S M S Ammonium Hydroxide conc U U U U S U M S S S U S U U S S S M S S S S U Ammonium Phosphate U S S S S S S S S S S S M S S S S S S M S S S Ammonium Sulfate U M S S S U S S S S S S S S...

Page 63: ... S U S U S S S S S S S S M S M U Ethyl Alcohol 95 S S S U S S M S S S S S U S U S S S M S S S U S M U Ethylene Dichloride S U U S M U U S U U U U U U U U S U S S Ethylene Glycol S S S S S S S S S S S S S U S S S S S S S S M S M S Ethylene Oxide Vapor S U U S U S S M S S S U S U S S S U Ficoll Hypaque M S S S S S S S S S S S S S S S S S S M S S S Hydrofluoric Acid 10 U U U M U U U S S M U S S S S M...

Page 64: ...de M S S S S S S S S S S S S S S S S S M S S S Lactic Acid 100 S M S U S S S M S S M S M S S S Lactic Acid 20 S S M S M S S S S S S S M S M S S S N Butyl Alcohol S S U S S M U S M S S S S M M S M S S N Butyl Phthalate S S U S S S S U U S U U U M U U S U S M M S U S N N Dimethylformamide S S S U S M S S S U S U S U U S S U U S M S S S U Sodium Borate M S S S S S S S S S S U S S S S S S S S S S M S ...

Page 65: ...S S M S Nitric Acid 95 U U U U U U U U U M U U U U M U U S U S S S Hydrochloric Acid 10 U U M S S S U S S S U U S U S S S S S S S S U M S S Hydrochloric Acid 50 U U U U S U U S M S U U M U U S S S S M S M U U M M Sulfuric Acid 10 M U U S S U U S S M U S S S S S S S S S S U U U S S Sulfuric Acid 50 M U U U S U U S S M U U S U U M S S S S S U U U M S Schwefelsäure konz M U U U U U M M U U S U U U M ...

Page 66: ...M S S S S S S S S S M S S S S S S S S S S 1 Polyethyleneterephthalate Key S Satisfactory M Moderate attack may be satisfactory for use in centrifuge depending on length of exposure speed involved etc Suggest testing under actual conditions of use U Unsatisfactory not recommended Performance unknown suggest testing using sample to avoid loss of valuable material CHEMICAL MATERIAL ALUMINUM ANODIC CO...

Page 67: ...trument which has been damaged through accident negligence failure to follow operating instructions the use of electric currents or circuits other than those specified on the plate affixed to the instrument misuse or abuse Thermo Fisher Scientific reserves the right to change alter modify or improve any of its instruments without any obligation whatever to make corresponding changes to any instrum...

Page 68: ...376 49 6184 90 6000 Italy 39 02 95059 552 Netherlands 31 76 579 55 55 Nordic Baltic Countries CIS 358 9 329 10200 Russia 7 812 703 42 15 Spain Portugal 34 93 223 09 18 Switzerland 41 44 454 12 22 UK Ireland 44 870 609 9203 China 86 21 6865 4588 86 10 8419 3588 India 91 22 6716 2200 Japan 81 45 453 9220 Other Asian Countries 852 2885 4613 Australia 61 39757 4300 New Zealand 64 9 980 6700 Countries ...

Page 69: ...Failure messages 8 3 H HOLD 4 5 I Imbalance indicator 4 5 L Location 2 2 M Mains Connections 2 5 Maintenace 7 1 O Open door 4 2 Operation 4 1 P Precautions v Preface iii Preselecting Speed RCF 4 3 Preselecting the temperature 4 4 R Rotor Installation 4 3 S Select run time 4 4 Service of Thermo Fisher Scientific 7 6 Set run time 4 5 Starting centrifuge program 4 5 Stopping the centrifugation progra...

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