Thermo Scientific iBright CL750 User Manual Download Page 1

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For Research Use Only. Not for use in diagnostic procedures.

iBright

™

CL750 Imaging System

USER GUIDE

For use with the iBright

™

CL750 Imaging System

Catalog Numbers

A44116

Publication Number

MAN0018652

Revision

C.0

Summary of Contents for iBright CL750

Page 1: ...esearch Use Only Not for use in diagnostic procedures iBright CL750 Imaging System USER GUIDE For use with the iBright CL750 Imaging System Catalog Numbers A44116 Publication Number MAN0018652 Revisio...

Page 2: ...mber 2019 Updated the components list see Table 1 on page 6 Added web link to interactive training demonstrations see Product description on page 6 Updated the emission filters for imaging in Universa...

Page 3: ...Connect account 10 Link to Connect using an existing instrument account 10 If the unit is off 10 If the unit is on and in sleep mode 11 If the unit is on and in active mode 11 Operate the iBright ima...

Page 4: ...Prepare for shipping 33 Invitrogen iBright SAE Software Solution for 21 CFR Part 11 Support 34 Security Audit e Signature SAE for the iBright Imager 34 Activate your license key 35 Enable SAE mode 35...

Page 5: ...the instrument 51 Safety information for instruments not manufactured by Thermo Fisher Scientific 51 Instrument safety 51 General 51 Physical injury 52 LED Light Emitting Diode 52 Electrical safety 5...

Page 6: ...create images while also providing on board software for image analysis To further analyze data the instrument can be integrated with the iBright Image Analysis Software available for desktop and Con...

Page 7: ...Available accessories Component Part number White transilluminator screen A33828 Sample stage A33829 High Power USB Wi Fi Module A26774 Product information Contents iBright CL750 Imaging System User...

Page 8: ...t using an existing instrument account on page 10 Create an instrument account and link to Connect using a cloud account 1 Touch to sign in to your instrument user account 2 Touch Get Started 3 Click...

Page 9: ...from the drop down menu 6 Enter the linking code and click on Send Your Connect account should now be linked to your instrument account PC using instrument Connect app 1 Touch PC 2 Launch the instrume...

Page 10: ...n Create an instrument account and link to Connect using a cloud account on page 8 If the unit is off 1 Ensure the unit is plugged in and turn on using the switch on the back of instrument The instrum...

Page 11: ...se drawer Touch Live View and a live view sample image will be displayed auto zoomed and auto focused If the unit is on and in active mode 1 If not already signed in touch to sign in to use or set up...

Page 12: ...sion 568 617 nm 2 Open drawer and place sample in the center of the viewing area 3 Close drawer Touch Live View to see a live view sample image Instrument automatically aligns and focuses on the sampl...

Page 13: ...ds Touch or Finger swipe in the segmented dial Touch the dial center box to select a preset exposure time or touch Custom to manually input a time The image preview updates in real time 4 Touch Enter...

Page 14: ...to a specific region of the sample 3 To adjust or set a manual exposure time select a method Touch or within dial Finger swipe in the segmented dial Touch the dial center box to select a preset exposu...

Page 15: ...es a series of short exposures and then renders a preview image and a recommended exposure time This is not a real image 4 Touch More options4Region of Interest Region of Interest is for directing Sma...

Page 16: ...e from the channel touch Remove Dye at the bottom left hand corner of the screen and then Confirm Channel refers to the type of signal you want to capture and the imaging conditions you want to use Ch...

Page 17: ...of the selected channels with the indicated exposure times Captured image s appears in viewport and automatically saves to the gallery 5 If an acceptable image touch Export Gallery or Analyze If an un...

Page 18: ...amma White and Black slider bars Channels and Layers Allow user to navigate between individual channels associated with a common image file pertains only to Chemi and Universal modes e g Channels memb...

Page 19: ...view Bottom image displays the color composite with channels displayed as different false colors overlaid on a black background Individual channels can be toggled on and off in the composite image by...

Page 20: ...ges are saved to a common gallery that houses all guest image files View images On the Welcome screen or mode drop down menu 1 Touch Gallery or 2 Toggle between Thumbnail view and List view to see sto...

Page 21: ...tions regarding your network The following information may be required to map a network drive Drive location Enter the IP address server name and file pathway use forward slashes Example 192 168 1 100...

Page 22: ...for your application From the Export images screen 1 Touch Choose File Type 2 Touch a file type File type Description Publication Exports 24 bit color adjusted images for data sharing and presentatio...

Page 23: ...ID and choose the desired username of the individual gallery that you want to manage 6 Touch Done In the filtered Gallery select the desired image file s or touch Select All to select all image files...

Page 24: ...adjustments Functions Adjust lanes Add remove or edit lanes Adjust bands Add remove or edit bands Add markers Identify a molecular weight lane for each analysis frame A known standard can be used to a...

Page 25: ...y 2 pixel area surrounding the identified band region Migration Distance Rf Distance migrated divided by the gel length c For TPN normalized data the lane and band table has the following additional o...

Page 26: ...e system can identify 1 4 different analysis frames 5 Optional Touch the Sensitivity dial to raise or lower the band find threshold to increase or decrease the number of identified bands and lanes in...

Page 27: ...a display language subject to availability Reset User Tips Reset user tips for the logged in guest Reset Factory Defaults Note Selecting this reset erases all user data and gallery files Resets instru...

Page 28: ...our Connect account Network Configuration Wired Searches for an ethernet connection Wireless Searches for a wireless connection Requires a USB wireless adapter High Power USB Wi Fi Module Product A267...

Page 29: ...log in to your user account Once you log in you are automatically taken to the Welcome screen Note You must be an Admin logged into your profile to manage accounts 2 Touch then click All Accounts All...

Page 30: ...f the testing Not applicable to iBright CL750 model Clean the instrument Repeated instrument use can cause spots and smudges on the sample stage which can then decrease image quality Clean the sample...

Page 31: ...as those currently in the iBright imager 1 Power off then unplug the iBright imager 2 Using a flat head screwdriver pry open the fuse door and remove the fuse holder 3 Remove each fuse from its fuse h...

Page 32: ...ting techniques when lifting or moving the instrument At least two people are required to lift it IMPORTANT Moving your instrument can create subtle changes in the alignment of the instrument optics R...

Page 33: ...ping 1 Touch 2 Touch Service Tools 3 Touch Ship prep mode Drawer opens 4 Remove turntable 5 Touch Next 6 Power off the instrument Maintain the instrument Move the instrument iBright CL750 Imaging Syst...

Page 34: ...er accounts Managing and enforcing password policies of all accounts Assigning managing and enforcing access rights to all accounts Documenting and maintaining audit and e Signature histories Permitti...

Page 35: ...lidation is successful Registration complete appears Note The license activation code is case sensitive 6 Press Done The activation code will be saved 7 Enter the IP address and port of the SAE consol...

Page 36: ...e then the following warning will pop up Note SAE users will not be able to access their current images when SAE is disabled You can backup or export those files before disabling SAE Image files remai...

Page 37: ...iBright instrument functions based on your user role set by the SAE Console Admin in the SAE Console SAE user roles Role Description SAE Console Administrator SAE Console Admin A default system role...

Page 38: ...ce Yes Yes No 1 Enabling SAE on board iBright instruments requires the Local Admin initiate and the SAE Console Admin approve Non SAE user roles Role Description Local Administrator Admin Local Admin...

Page 39: ...allery by file status 1 To filter or sort image files in the iBright Gallery by file status touch Filter options 2 Check the appropriate boxes X Uncommitted Image file s with uncommitted changes Commi...

Page 40: ...ctively Commit changes In SAE mode user changes acquisition analysis adjustments editing renaming and export are recorded in the audit history for each image file After image acquisition the file is a...

Page 41: ...he Sign Data button is also available under More options in the acquisition analyze and adjust workflows 2 Select the Meaning for signing the file from the drop down menu IMPORTANT If more than one e...

Page 42: ...lowing information Version Date Time Username Role Action Reason and Comment Audit history is also available in the Adjust and Analyze workflows under More options 2 Select one row in the audit histor...

Page 43: ...alysis Software Secure Note Uncommitted files cannot be exported Note g2i files can be exported as signed or unsigned files based on the file status Generate and export Analysis Report Touch Generate...

Page 44: ...space between the wall and the equipment so the power cord can be disconnected in case of emergency Electric receptacle with grounding capability Maximum power dissipation 960 W not including computer...

Page 45: ...ed on the actual current draw of the instrument is expected to be approximately 960 W 3275 Btu h Vibration Ensure that the instrument is not adjacent to strong vibration sources such as a centrifuge p...

Page 46: ...cientific service representative is to install the instrument If the instrument will be connected to a LAN an active tested network jack must be in place before the scheduled installation date A repre...

Page 47: ...ment and computer connections iBright Imager front view 1 2 3 1 Touchscreen 2 USB port 3 Sample stage Instrument specifications Instrument and computer connections iBright CL750 Imaging System User Gu...

Page 48: ...t 2 Ethernet port RJ45 port for 10 100 Mbps ethernet communication with the instrument 3 Fuse cover 4 Power switch 5 Power port 100 240 VAC Instrument specifications Instrument and computer connection...

Page 49: ...Symbols on this instrument Symbols may be found on the instrument to warn against potential hazards or convey important safety information In this document the hazard symbol is used along with one of...

Page 50: ...r les d chets municipaux pour les dispositions d limination et communiquer avec le service la client le pour des renseignements sur les options d limination responsable Safety alerts on this instrumen...

Page 51: ...n for the information needed for the safe use of these products Instrument safety General CAUTION Do not remove instrument protective covers If you remove the protective instrument panels or disable i...

Page 52: ...a causing permanent blind spots To ensure safe LED operation Never look directly into the light beam Wear proper eye protection and post a warning sign at the entrance to the laboratory if the LED pro...

Page 53: ...lors que le dispositif de mise la terre est d connect La continuit de la mise la terre est imp rative pour le fonctionnement de l instrument en toute s curit WARNING Power Supply Line Cords Use proper...

Page 54: ...nt destin e aux utilisateurs L op rateur ou toute autre personne responsable est tenu d assurer le respect des exigences suivantes Ne pas utiliser d agents de nettoyage ou de d contamination susceptib...

Page 55: ...cal equipment for measurement control and laboratory use Part 2 081 Particular requirements for automatic and semi automatic laboratory equipment for analysis and other purposes IEC 62471 1 2007 Photo...

Page 56: ...terference in which case the user will be required to correct the interference at his own expense Environmental design Reference Description EU Directive 2012 19 EU European Union WEEE Directive Waste...

Page 57: ...entration of the hazardous substance in all homogeneous materials for the part is below the relevant threshold of the GB T 26572 standard X GB T26572 Indicates that the concentration of the hazardous...

Page 58: ...ontainer storage After emptying a waste container seal it with the cap provided Characterize by analysis if necessary the waste generated by the particular applications reagents and substrates used in...

Page 59: ...sentant un danger biologique ou radioactif exigent parfois une manipulation sp ciale et des limitations peuvent s appliquer leur limination Biological hazard safety WARNING BIOHAZARD Biological sampl...

Page 60: ...Imager iBright Analysis Software Secure iBright Analysis Software Secure desktop version supports 21 CFR Part 11 compliance SAE Administrator Console Software SAE Administrator Console Software suppor...

Page 61: ...fety Data Sheets SDSs also known as MSDSs Note For SDSs for reagents and chemicals from other manufacturers contact the manufacturer Limited product warranty Life Technologies Corporation and or its a...

Page 62: ...0 Instrument User Guide_UG_MAN0018652 v3 GUID A95B1212 50E5 4547 94AC D070C8471F1C 2020 07 21 18 10 35 en 17 12 14 381 01 00 thermofisher com support thermofisher com askaquestion thermofisher com 24...

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