IonPac CS14 Manual
Doc. No. 034848-10
Page 36 of 54
5.17 Methanesulfonic Acid Gradient Elution of Alkanolamines with Ammonium, Group I & II
Cations
The determination of alkanolamines is of great interest since these compounds are widely used in chemical and pharmaceutical
industries. They are used for the production of emulsifying agents, corrosion inhibitors, laundry materials, dyes, medicines
and gas purification. Alkanolamines are increasing in use as replacements for hydrocarbon-based solvents in coating and
plating systems.
Alkanolamines are not normally present in waste waters. If present in the biofeed water (water entering the biopond) the
bacteria will be killed, upsetting the biobalance in the biopond. NPDES discharge limits would probably be violated.
The determination of monoethanolamine, diethanolamine and other alkanolamines in the presence of ammonia, alkali and
alkaline earth metals can be accomplished by gradient elution using 5 mM methanesulfonic acid (MSA) as the initial eluent
and then stepping to 21 mM methanesulfonic acid (MSA) after 12 minutes. The CSRS ULTRA can be used in the
AutoSuppression Recycle Mode.
Sample Loop Volume:
6.25 µL (2-mm), 25 µL (4-mm)
Analytical Column:
IonPac CS14 Analytical Column
Eluent:
See Chromatogram
Eluent Flow Rate:
0.25 mL/min (2-mm), 1.0 mL/min (4-mm)
SRS Suppressor:
Cation Self-Regenerating Suppressor, CSRS ULTRA (2-mm or 4-mm)
Recycle Mode
or MMS Suppressor:
Cation MicroMembrane Suppressor, CMMS III (2-mm or 4-mm)
MMS Regenerant:
100 mN TBAOH
(tetrabutylammonium hydroxide)
Expected Background Conductivity:
< 1 µS
Storage Solution:
Eluent
Eluent 1:
Type I DI Water
Eluent Flow Rate:
1.0 mL/min
Eluent 2:
10 mM MSA
(4-mm System)
Eluent 3:
100 mM MSA
TIME (min)
%E1
%E2
%E3
C o m m e n t s
Equilibration
0.0
90
10
0
Equilibrate to 1.0 mM MSA
7.0
90
10
0
Analysis
0.0
90
10
0
Inject, 1.0 mM MSA
0.1
90
10
0
Inject Valve to Load Position
16.0
70
30
0
3.0 mM MSA
30.0
82
0
18
18.0 mM MSA
30.1
90
10
0
1.0 mM MSA
