IonPac CS14 Manual
Doc. No. 034848-10
Page 10 of 54
E.
Reconnect the CTC.
Connect the CTC to the eluent line that is connected to the injection valve.
The background conductivity of your system should be less than 3 µS when 10 mM methanesulfonic acid (MSA) is being
pumped through the chromatographic system with the CSRS in-line and properly functioning. The baseline shift should be no
greater than 1 µS during a gradient concentration ramp from 10 to 40 mM methanesulfonic acid (MSA). If the baseline shifts
are greater than 5 µS, the CTC should be cleaned using steps A - E above.
Flush the CTC at the end of each operating day
. This removes any impurities that may have accumulated on it. This will
minimize periodic maintenance and lost data.
A.
Disconnect the CTC.
It should be installed between the injection valve
B. Direct the outlet of the CTC to a separate waste container.
C.
Flush the CTC.
Use 30 mL of a 10X eluent concentrate of the strongest eluent required by the application at a flow
rate of 2.0 mL/min.
D.
Flush the CTC prior to start-up.
Prior to the use of the chromatographic system on the next day, flush the CTC with
30 mL of the strongest eluent used in the gradient program.
3.3
The Injection Loop
Table 4
Smallest Injectable Volumes (
μ
L)
Valve Type
Using 0.012" ID
Tefzel Tubing
Using 0.007" ID
Tefzel Tubing
Using 0.010" ID
PEEK Tubing
Using 0.005" ID
PEEK Tubing
Dionex
BF2 Valve
(8 µL Internal Volume)
(10 cm Loop)
15.2
10.5
13.1
9.2
Dionex
MicroInject Valve
(10.5 µL Internal Volume)
(14 cm Loop)
20.5
14.0
17.6
12.2
Rheodyne
Microinjection Valve
Model 9126
(0.8 µL Internal Volume)
(10 cm Loop)
8.0
3.3
5.9
2.0
