IonPac CS14 Manual
Doc. No. 034848-10
Page 53 of 54
Column Cleanup Procedure for Polyvalent Cations and Acid-Soluble Contaminants or Transition Metals
A. Prepare 500 mL of 1 M HCl for the cleanup solution. Alternatively prepare 500 mM oxalic acid to remove transition
metals such as iron or aluminum contamination.
Nitric acid should not be used instead of hydrochloric acid since nitric acid will not effectively remove
iron contaminants. Do not clean the column with alcohols or with basic eluents.
B.
Disconnect the suppressor from the IonPac CS12 Analytical Column. If your system is configured with both a guard
column and an analytical column, place the guard behind the analytical column in the eluent flow path. Double check
that the eluent flows in the direction designated on each of the column labels.
When cleaning an analytical column and a guard column in series, ensure that the guard column is
placed after the analytical column in the eluent flow path. Contaminants that have accumulated on the
guard column can be eluted onto the analytical column and irreversibly damage it. If in doubt, clean
each column separately.
C.
Set the pump flow rate to 1.0 mL/min for a CS12 4-mm Analytical or Guard Column or set the pump flow rate to 0.25
mL/min for a CS12 2-mm Analytical or Guard Column.
D.
Rinse the column for 15 minutes with 10 mM HCl before pumping the chosen cleanup solution over the column.
E.
Pump the cleanup solution (1 M HCl or 500 mM oxalic acid) through the column for 60 minutes.
F.
Rinse the column for 15 minutes with 10 mM HCl before pumping eluent over the column.
G.
Equilibrate the column(s) with eluent before resuming normal operation for at least 30 minutes.
H. Reconnect the suppressor to the CS12 Analytical Column and place the guard column in line between the injection
valve and the analytical column if your system was originally configured with a guard column.
Do not pump HCl through the CSRS ULTRA when used in the electrolytic mode.
Hydrophobic Cations and Organic Contaminants
A. Disconnect the analytical column from the injection valve and the suppressor. Disconnect the Gradient Mixer or the
Cation Trap Column (CTC-1) from the gradient pump. Connect the IonPac CS12 2-mm or 4-mm Analytical Column
directly to the gradient pump. Direct the effluent from the analytical column directly to a waste container.
B.
Set the flow rate to 1 mL/min on 4-mm systems or 0.25 mL/min on 2-mm systems.
CAUTION
CAUTION
NOTE
