IonPac CS14 Manual
Doc. No. 034848-10
Page 35 of 54
5.16 Elution of Substituted Methyl- and Ethylaminoethanols
Two fairly hydrophilic amino alcohols, methylaminoethanol and ethylaminoethanol, can be determined in the presence of
ammonia, Group I & II cations by isocratic elution using 10 mM trifluoroacetic acid (TFA) with 1.0 to 1.5 % acetonitrile.
Trifluoroacetic acid is used in this separation instead of methanesulfonic acid as the source of hydronium ions. Since
trifluoroacetic acid is a weaker acid than methanesulfonic acid, slightly higher concentrations have the equivalent eluting
power of methanesulfonic acid.
Sample Loop Volume:
4.5 µL (2-mm), 18 µL (4-mm)
Analytical Column:
IonPac CS14 Analytical Column
Eluent:
See Chromatogram
Eluent Flow Rate:
0.25 mL/min (2-mm), 1.0 mL/min (4-mm)
SRS Suppressor:
Cation Self-Regenerating Suppressor, CSRS ULTRA (2-mm or 4-mm)
External Water Mode
or MMS Suppressor:
Cation MicroMembrane Suppressor, CMMS III (2-mm or 4-mm)
MMS Regenerant:
100 mN TBAOH
(tetrabutylammonium hydroxide)
Expected Background Conductivity:
< 1 µS
Storage Solution:
Eluent
Isocratic Eluent: 10 mM TFA / 1.0% ACN
Figure 15
Elution of Substituted Methyl- and Ethylaminoethanols
Analyte
mg/L
1.
Li
+
0.5
2.
Na
+
2.0
3.
NH
4
+
2.5
4.
2-Ethylaminoethanol
10.0
5.
K
+
5.0
6.
2-Diethylaminoethanol
20.0
7.
Mg
2+
2.5
8.
Ca
2+
5.0
1 mg/L = 1 ppm
Analyte
mg/L
1.
Li
+
0.5
2.
Na
+
2.0
3.
NH
4
+
2.5
4.
2-Methylaminoethanol
10.0
5.
2-Dimethylaminoethanol
20.0
6.
K
+
5.0
7.
Mg
2+
2.5
8.
Ca
2+
5.0
1 mg/L = 1 ppm
Isocratic Eluent: 10 mM TFA / 1.5% ACN
0
5
10
15
μ
S
0
Minutes
1
2
3
4
5
5
7
8
6
0
5
10
15
μ
S
0
Minutes
1
2
3
4
5
5
7
8
6
