IonPac CS14 Manual
Doc. No. 034848-10
Page 13 of 54
SECTION 4 - OPERATION
4.1
General Operating Conditions
Sample Volume:
2-mm: 2.5 µL Loop + 0.8 µL Injection valve dead volume
4-mm: 25 µL Loop + 0.8 µL Injection valve dead volume
Column:
2-mm: CS14 2-mm Analytical Column (+ CG14 2-mm Guard Column)
4-mm: CS14 4-mm Analytical Column (+ CG14 4-mm Guard Column)
Eluent:
10 mN MSA
Eluent Flow Rate:
2-mm: 0.25 mL/min
4-mm: 1.0 mL/min
SRS Suppressor:
Cation Self-Regenerating Suppressor, CSRS ULTRA (2-mm or 4-mm)
AutoSuppression Recycle Mode
or MMS Suppressor:
Cation MicroMembrane Suppressor, CMMS III
(2-mm or 4-mm)
MMS Regenerant:
100 mN tetrabutylammonium hydroxide (TBAOH)
Use Dionex Cation Regenerant Solution (P/N 039602).
or AES Suppressor:
Cation Atlas Electrolytic Suppressor, CAES
(up to 25 µeq/min)
Expected Background Conductivity:
< 3 µS
Storage Solution:
Eluent
4.2
Operating Precautions
IonPac CS14 Operation Precautions
Operate below 4,000 psi (27.57 MPa)
Filter and Degas Eluents
Filter Samples
4.3
Chemical Purity Requirements
Obtaining reliable, consistent and accurate results requires eluents that are free of ionic impurities. Chemicals, solvents and
deionized water used to prepare eluents must be of the highest purity available. Low trace impurities and low particle levels in
eluents also help to protect your ion exchange columns and system components. Dionex cannot guarantee proper column
performance when the quality of the chemicals, solvents and water used to prepare eluents has been compromised.
4.3.1 Deionized Water
The deionized water used to prepare eluents should be Type I Reagent Grade Water with a specific resistance of 18.2 megohm-
cm. The deionized water should be free of ionized impurities, organics, microorganisms and particulate matter larger than 0.2
µm Bottled HPLC-Grade Water (with the exception of Burdick & Jackson) should not be used since most bottled water
contains an unacceptable level of ionic impurities.
4.3.2 Inorganic Chemicals
It is very important for the eluent to be as free of impurities as possible. The chemicals and water required to prepare the eluent
should be of the highest purity available. Use deionized water with a specific resistance of 18.2 megohm-cm.
A. Use degassed Type I deionized water with a specific resistance of 18.2 megohm-cm to make all standards, eluents and
regenerants.
CAUTION
