IonPac CS14 Manual
Doc. No. 034848-10
Page 29 of 54
5.12 Elution of Aliphatic Monoamines
The determination of aliphatic monoamines in the presence of ammonia, Group I & II cations can be accomplished by isocratic
elution using 40 mM methanesulfonic acid (MSA) if complete resolution of ammonia, Group I & II cations is not required. The
determination of all of the components requires a step gradient from 6 mM methanesulfonic acid (MSA) with 2% acetonitrile
(ACN) to 8 mM methanesulfonic acid (MSA) with 10% acetonitrile (ACN) at 9 minutes. Note that with the addition of solvent,
the amines move forward in the chromatogram relative to magnesium and calcium.
Sample Loop Volume:
6.25 µL (2-mm), 25 µL (4-mm)
Analytical Column:
IonPac CS14 Analytical Column
Eluent:
See Chromatogram
Eluent Flow Rate:
0.25 mL/min (2-mm), 1.0 mL/min (4-mm)
SRS Suppressor:
Cation Self-Regenerating Suppressor, CSRS ULTRA (2-mm or 4-mm)
Recycle Mode or External Water Mode
or MMS Suppressor:
Cation MicroMembrane Suppressor, CMMS III (2-mm or 4-mm)
MMS Regenerant:
100 mN TBAOH
(tetrabutylammonium hydroxide)
Expected Background Conductivity:
< 1 µS
Storage Solution:
Eluent
Eluent 1:
Type I DI Water
Eluent Flow Rate:
1.0 mL/min
Eluent 2:
100 mM MSA
(4-mm System)
Eluent 3:
20% Acetonitrile
TIME (min)
%E1
%E2
%E3
C o m m e n t s
Equilibration
0.0
84
6
10
Equilibrate to 6.0 mM MSA / 2.0% ACN
7.0
84
6
10
Analysis
0.0
84
6
10
Inject, 6.0 mM MSA / 2.0% ACN
0.1
84
6
10
Inject Valve to Load Position
9.0
84
6
10
6.0 mM MSA / 2.0% ACN, step change to
9.1
42
8
50
8.0 mM MSA / 10% ACN
20.0
42
8
50
8.0 mM MSA / 10% ACN
