5
–
Troubleshooting
Thermo Scientific
Product Manual for IonPac AS31 Columns
Page 32 of 45
155007-01
For Research Use Only. Not for use in diagnostic procedures.
Table 5
Dionex IonPac AG31/AS31 Column Spare Parts
Bed Support Assembly
044689
End Fitting
043278
5.1.3
Filter Eluent
Eluents containing particulate material or bacteria may clog the column inlet bed support. Filter
water used for eluents through a 0.45 µm filter.
5.1.4
Filter Samples
Samples containing particulate material may clog the column inlet bed support. Filter samples
through a 0.45 µm filter prior to injection.
5.2
High Background
In a properly working system, the background conductivity level for the standard eluent system
is shown below.
Table 6
Typical Background Conductivity for Anion Columns
Eluent
Expected Background Conductivity
17 mM KOH
0.5 – 0.8 µS
85 mM KOH
0.8 – 1.5 µS
60 mM KOH/15% Methanol
1-3 µS
5.2.1
Preparation of Eluents
A.
Make sure that the eluents prepared manually and the regenerant are made correctly.
B.
Make sure that the eluents are made from chemicals with the recommended purity.
C.
Make sure that the deionized water used to prepare the reagents has a specific resistance of
18.2 megohm-cm.
5.2.2
A Contaminated Trap Column
Please refer to the Product Manual for the Trap column in use.
5.2.3
Contaminated Dionex CR-ATC 500 or CR-ATC 600 Trap Column
A.
A Dionex CR-ATC 500 or CR-ATC 600 Trap Column should be installed if using a Dionex
Eluent Generator Cartridge.
B.
If there is high baseline shift during gradient, the Dionex CR-ATC 500 or CR-ATC 600 may
be contaminated or too old, please replace Dionex CR-ATC 500 or CR-ATC 600 and/or refer
to the Clean-Up Procedure, in the Dionex CR-TC Product Manual (Document No. 079684).