4
–
Example Applications
Thermo Scientific
Product Manual for IonPac AS31 Columns
Page 21 of 45
155007-01
For Research Use Only. Not for use in diagnostic procedures.
4.4
Separation of Various Inorganic and Organic Anions
The Dionex IonPac AS31 column provides excellent separation of a variety of environmental anions including
inorganic anions, oxyhalides, oxyanions, and organic acids. When developing a method, keep in mind that if there is
a big difference in the hydrophobicity and valencies of the two analytes, variation in column capacities will have
significant effect on the peak resolution.
Figure 3
Separation of Various Inorganic and Organic Anions Using the Dionex IonPac
AS31 Column
Column:
Dionex IonPac AG31/AS31, 2mm
Eluent:
15 mM KOH from 0 to 6 minutes,
15-70 mM KOH from 6 to 26 minutes
Eluent Source:
Dionex EGC 500 KOH cartridge
Flow Rate:
0.30 mL/min
Inj. Volume:
2.5 µL
Temperature:
15˚C
Detection:
Suppressed Conductivity,
Dionex ADRS 600 (2 mm)
AutoSuppression, recycle mode
Peaks (Standard):
mg/L
1.
Fluoride
3.0
2.
Acetate
10.0
3.
Formate
10.0
4.
Chlorite
10.0
5.
Bromate
10.0
6.
Chloride
10.0
7.
Sulfate
10.0
8.
Malonate
10.0
9.
Oxalate
10.0
10. Nitrite
10.0
11. Phosphate
10.0
12. Arsenate
20.0
13. Citrate
20.0
14. Chlorate
20.0
15. Bromide
20.0
16. Chromate
20.0
17. Nitrate
20.0
0
2
4
6
8
10
12
14
16
18
20
22
24
26
-1
10
1
2
3
4
5
6
7
8
9 10
11
12
13
14
15
16
17
Minutes
µS
