5
–
Troubleshooting
Thermo Scientific
Product Manual for IonPac AS31 Columns
Page 31 of 45
155007-01
For Research Use Only. Not for use in diagnostic procedures.
E.
The Dionex Dynamically Regenerated Suppressor with backpressure loops may add up to
100 psi (0.69 MPa). No other components should add more than 100 psi (0.69 MPa) of
pressure. Refer to the appropriate manual for cleanup or replacement of the problem
component.
F.
A Dionex High-Pressure In-Line Filter positioned between the Pump and Eluent Generator
(or injection valve if an EG is not installed) should be installed to prevent particulates from
blocking the system.
5.1.2
Replacing Column Bed Support Assemblies
If the column or guard inlet bed support is determined to be the cause of the high back pressure,
it should be replaced. To change the inlet bed support assembly, refer to the following instructions,
using one of the two spare inlet bed support assemblies included in the Ship Kit.
If the column tube end is not clean when inserted into the end fitting, particulate matter
may obstruct a proper seal between the end of the column tube and the bed support
assembly. If this is the case, additional tightening may not seal the column but instead
damage the column tube or the end fitting.
Replacement of the 2 mm outlet bed support is not recommended.
A.
Disconnect the column from the system.
B.
Carefully unscrew the inlet (top) column fitting. Use two open-end wrenches.
C.
Remove the bed support. Turn the end fitting over and tap it against a bench top or other hard,
flat surface to remove the bed support and seal assembly. If the bed support must be pried
out of the end fitting, use a sharp pointed object such as a pair of tweezers, but be careful that
you do not scratch the walls of the end fitting. Discard the old bed support assembly.
D.
Place a new bed support assembly into the end fitting. Make sure that the end of the column
tube is clean and free of any particulate matter so that it will properly seal against the bed
support assembly. Use the end of the column to carefully start the bed support assembly into
the end fitting.
E.
Screw the end fitting back onto the column. Tighten it finger-tight, then an additional 1/4 turn
(25 in x lb). Tighten further only if leaks are observed.
F.
Reconnect the column to the system and resume operation.
CAUTION
!
NOTE
!
