2
–
Installation
Thermo Scientific
Product Manual for IonPac AS31 Columns
Page 12 of 45
155007-01
For Research Use Only. Not for use in diagnostic procedures.
2.4
Eluents
2.4.1
Eluent Generation
It is recommended that Dionex IonPac columns are used with Dionex HPIC Systems equipped
with a Thermo Scientific Dionex Eluent Generator (EG). The use of eluent generation provides a
more stable baseline, improved performance, and increased reproducibility over the use of
manually prepared eluents.
The Dionex Eluent Generator is used to automatically produce eluents either isocratically or as
gradients from deionized water. Please refer to the Dionex EG manual for information on the
operation of the Dionex eluent generators.
Only Dionex Eluent Generator Cartridges (EGCs) rated for 5,000 psi or higher should be
used when running Dionex IonPac AS31 column due to the higher backpressures
generated at typical operational flow rates.
2.5
Dionex IonPac Guard Columns
A Dionex IonPac AG31 Guard Column is normally used with the Dionex IonPac AS31 Analytical
Column. A guard is placed in front of the analytical column to prevent sample contaminants from
damaging the analytical column. It is easier to clean or replace the guard column than it is the
analytical column. Placing a guard column in front of the analytical column will cause retention
times to increase by approximately 1.5% under isocratic test conditions. Replacing the guard
column at the first sign of peak efficiency loss or decreased retention time will prolong the life of
the analytical column.
2.6
Trap Columns
For gradient operation a trap column is installed between the gradient pump and the injection
valve and takes the place of the gradient mixer if present. The trap column is filled with high
capacity ion exchange resin which helps to minimize the baseline shift caused by increasing ionic
contaminants as the eluent ionic strength increases over the course of gradient analysis.
2.6.1
Continuously Regenerated Anion Trap Columns for Use with Eluent Generator Cartridges
For applications using eluent generation on a Dionex ICS-5000
+
HPIC system, a Dionex CR-ATC
500 Continuously Regenerated Anion Trap Column (Item # 075550) should be installed at the
Dionex EGC 500 eluent outlet to remove trace level ionic contaminants from the carrier deionized
water. For applications using eluent generation on a Dionex ICS-6000 HPIC system, use a Dionex
CR-ATC 600 (Item # 088662). See the Dionex Continuously Regenerated Trap Column Product
Manual (Document No. 079684) for instructions.
As an alternative to a Dionex CR-ATC 500, a Thermo Scientific Dionex ATC 500 Trap Column
(Item # 079018) can be installed between the pump outlet and the inlet of the Dionex EGC 500 to
remove anionic contaminants from the carrier deionized water. Note that use of the Dionex ATC
500 will require off-line regeneration. See the Dionex Anion Trap Columns Product Manual
(Document No. 032697) for instructions.
CAUTION
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