4
–
Example Applications
Thermo Scientific
Product Manual for IonPac AS31 Columns
Page 19 of 45
155007-01
For Research Use Only. Not for use in diagnostic procedures.
4.2
Isocratic Elution With and Without a Guard Column
Isocratic elution of inorganic anions on the Dionex IonPac AS31 column has been optimized utilizing a hydroxide
eluent. By using this eluent, common inorganic anions can be used to test the performance (QAR) of the Dionex IonPac
AS31 column. The Dionex IonPac AS31 analytical column should always be used with the Dionex IonPac AG31 guard
column. An operating temperature of 15 °C is used to ensure reproducible resolution and retention. Note that the Dionex
IonPac AG31 guard column is packed with a microporous resin of proportionally lower capacity and contributes
approximately 1.5% increase in retention time when placed in-line prior to the analytical column under isocratic test
conditions.
Figure 1
Dionex IonPac AS31 With and Without Dionex IonPac AG31 Guard Column
Column:
See Chromatogram
Eluent:
24 mM KOH
Eluent Source:
Dionex EGC 500 KOH cartridge
Flow Rate:
0.30 mL/min
Inj. Volume:
2.5 µL
Temperature:
15˚C
Detection:
Suppressed Conductivity,
Dionex ADRS 600 (2 mm)
AutoSuppression, recycle mode
Peaks (Standard):
mg/L
1.
Fluoride
5.0
2.
Sulfate
10.0
3.
Chloride
10.0
4.
Bromide
20.0
5.
Thiosulfate
20.0
6.
Nitrate
20.0
Dionex IonPac AS31
Dionex IonPac AG31 + AS31
0
5
10
15
20
25
30
-1
10
1
2
3
4
5
6
Minutes
µS
-1
10
1
2
3
4
5
6
µS
