77210163GB Issue 3
42
4.5 TO REMOVE ThE SPECIMEN fROM ThE CRYOCASSETTE
When the last section is cut, lock the Handwheel at the 3 o’clock position. Remove the
cryocassette from the clamp.
If the specimen is to be stored frozen, wrap it in metal foil before putting it into a freezer.
Do not store it in the Refrigerated Chamber of the Cryotome.
If the specimen is to be fixed, allow it to thaw before placing it in a suitable fixative;
otherwise discard it by the usual method for unfixed tissue. Brush away any debris that
has collected around the Cassette Clamp or Head.
Decontaminate and clean the Cryocassette.
4.6 hOUSEKEEPING
Daily:
Decontaminate the specimen brush by putting it overnight
in a solution of 10% Formalin. Wash and dry the brush
each morning before use.
After fumigation:
Carefully remove the debris tray, Knife Holder and any
debris. Wash in running hot water initially to remove
the debris, then immerse overnight in a solution of 10%
Formalin. The following day, wash, dry, and replace in the
Cryotome.
Wash the Refrigerated Chamber with alcohol or warm
soapy water to remove debris, and rinse the outlet tube in
the bottom of the chamber, and the grill beneath the fins of
the heat exchanger with a solution of 10% Formalin.
ALwAYS REfER TO MATERIAL SAfETY DATA ShEET (MSDS) fOR
REAGENTS SPECIfIED IN APPENDIX B
Summary of Contents for Cryotome
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