CO
2
Incubator BB 150 Operating Instructions
10
1.
General notes
1.4
Intended purpose of the device
1.4.1
Correct use
The CO
2
incubator BB 150 is a laboratory device for preparing and cultivating cell
and tissue cultures. The device allows the simulation of the special physiolo-
gical ambient conditions for these cultures due to the exact control of:
• temperature,
• CO
2
content,
• an increased relative humidity.
The BB 150 has been designed for installation and operation in the following fields
of application:
• Laboratories for cytobiological and biotechnological experiments of safety
levels L1, L2, and L3.
• Medical-microbiological laboratories in accordance with DIN 58 956.
• Laboratories in the central area of clinics and hospitals.
The CO
2
required for the incubator is supplied to the device from a separate gas
supply system, either from gas cylinders or from a central pressurized gas con-
tainer.
The layout of the gas supply system must ensure that the operating pressure
of the gas supply lines can be set to a range between 0.8 bar (min.) to 1 bar (max.)
and that the pressure cannot be changed.
Depending on the capability of the gas supply system, several devices may be
connected to one gas cylinder.
The CO
2
incubator is suited for continuous operation.
1.4.2
Incorrect use
Do not use cell or tissue cultures in the device that are not in accordance with
the regulations of safety levels L1, L2, and L3.
Do not use tissues, substances or liquids that:
• are easily ignitible or explosive,
• release vapors that form combustible or explosive mixtures when exposed
to air,
• release poisons.
1.5
Standards and directives
The device is in accordance with the following standards and guidelines:
• DIN EN 61010
• Low Voltage Guideline 73/23 EWG
• EMC Guideline 89/336 EWG
• UVV VBG 20
• DIN 12880 Part 1/11.78
