Gate count results
Gating screen
The
Gating
screen for cell fluorescence assays contains the controls for gating count results based on
size, brightness, circularity, and fluorescence intensity. You can adjust the count parameters before or
after performing a count, and save these changes to the current template or as a separate template
(“Save as new template”
).
Gate count results
1.
On the
Results
screen, press
Gating
to open the count parameters screen containing the controls
for adjusting the count parameters in the selected channel.
Note:
For a description of the count parameters and count parameter controls (i.e., parameter
sliders) see “Count parameters”
2.
(Optional)
: Press
Histogram
to view the distribution of cells based on size or fluorescence
intensity. See “View results”
.
3.
Press
BF
(brightfield) to adjust the parameters for size, brightness, and circularity using the gating
sliders.
4.
Select the desired fluorescence channel (e.g., DAPI, TX Red, etc.) to adjust the threshold for
fluorescence intensity using the fluorescence intensity slider.
Note:
The fluorescence channels available depend on the EVOS
™
light cubes installed in the
instrument.
5.
When finished, press
Apply
to save the changes to count parameters and return to the
Results
screen OR press
Cancel
or
Back
to return to the
Results
screen without saving the changes.
6.
To save the parameter changes to the current template or to create a template using the new count
parameter, see “Edit and save a new template”
.
Chapter 6
Fluorescence assays
Gate count results
6
36
Countess
™
3 FL Automated Cell Counter User Guide
Summary of Contents for AMQAF2000
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