View results
Results screen for brightfield
The Results screen for cell count and cell viability assays performed using the brightfield channel
displays a composite image of the objects counted and the results of the cell count and cell viability
calculations (total concentration, percentage and concentration of live and dead cells).
Note:
When performing cell counts and cell viability assays in brightfield, the counting algorithm
assumes that you have diluted your cells 1:1 in trypan blue and takes this dilution into account when
calculating the total cell concentration. The cell concentration displayed in the Results screen is the
original cell concentration before dilution into trypan blue.
Identify objects counted
Results screen
The
Results
screen allows you to identify the objects (i.e., cells) counted in each channel and included
in the count results for further review. After reviewing the marked objects, you can adjust the threshold
for size, brightness, and/or circularity as desired for your application. See “Gate count results”
.
Chapter 5
Cell count and cell viability assays
View results
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26
Countess
™
3 FL Automated Cell Counter User Guide
Summary of Contents for AMQAF2000
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