3.
Let the sample mixture settle in the chamber for 30 seconds, and then insert the slide into the slide
adapter. You will hear a soft click once the slide is pushed in correctly.
Note:
The instrument reads one side of the slide at a time, so insert the sides appropriately.
Note:
The instrument is shipped with the slide adapter already installed.
4.
Use the touchscreen to run the sample count. See Chapter 5, “Cell count and cell viability assays”.
5.
To remove the slide, push the slide gently into the instrument until it clicks and the slide springs
out. Grasp the slide and pull it out the rest of the way.
Note:
After using the Countess
™
Cell Counting Chamber Slides, appropriately dispose of them as
biohazardous waste.
Do not reuse the disposable chamber slides.
6.
(Optional)
To remove the slide adapter, gently squeeze the tabs and pull the adapter completely out
of the instrument.
Load the Countess
™
Reusable Slide
1.
Before loading your sample into the Countess
™
Reusable Slide, place a cover slip on the counting
chamber, making sure the cover slip is clean and free of grease.
2.
Prepare the sample by adding 10 µL of your cell suspension to 10 µL of 0.4% trypan blue stain
(for brightfield only). Mix the sample mixture well by pipetting it up and down a few times. For a
fluorescence sample, only add 10 µL of your sample neat.
Chapter 4
Slide preparation and operation
Load the Countess
™
Reusable Slide
4
Countess
™
3 FL Automated Cell Counter User Guide
21
Summary of Contents for AMQAF2000
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